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71.
Recombinant H chain ferritins bearing site-directed amino acid substitutions at their ferroxidase centres have been used to study the mechanism of catalysis of Fe(II) oxidation by this protein. UV-difference spectra have been obtained at various times after the aerobic addition of Fe(II) to the recombinants. These indicate that the first product of Fe(II) oxidation by wild type H chain apoferritin is an Fe(III) mu-oxo-bridged dimer. This suggests that fast oxidation is achieved by 2-electron transfer from two Fe(II) to dioxygen. Modelling of Fe(III) dimer binding to human H chain apoferritin shows a solvent-accessible site, which resembles that of ribonucleotide reductase in its ligands. Substitution of these ligands by other amino acids usually prevents dimer formation and leads to greatly reduced Fe(II) oxidation rates. 相似文献
72.
Aqueous polymer two-phase systems containing dextran T-500 and PEG 4000 can be prepared which are biphasic below 18 degrees C and monophasic at higher temperatures. Both liganded and unliganded forms of glutamate dehydrogenase and troponin, which have similar partition coefficients if the protein is added to a two-phase system at 4 degrees C, have widely differing partition coefficients if added to the same system in the monophasic state at 20 degrees C and subsequently cooled to 4 degrees C. 相似文献
73.
Radioactive iron incorporated into rat liver ferritin in vivo and then released in vitro up to 24h after injection follows 'last-in-first-out' behaviour. Thus, within this period at least, the added iron does not equilibrate with iron already present in the molecule's iron-core. 相似文献
74.
Immunoreactivities of peptides purified after cleavage of human liver apoferritin are reported and discussed in relation to the known 3-dimensional and primary structures of homologous apoferritins. These studies point to 3 antigenic sites occupying continuous inter-helical regions of the polypeptide chains which lie on the surface of the apoferritin molecule. Other antigenic regions may encompass amino acids remote in the primary structure or belonging to different subunits. 相似文献
75.
Structural and functional studies on ferritins 总被引:1,自引:0,他引:1
P M Harrison G C Ford D W Rice J M Smith A Treffry J L White 《Biochemical Society transactions》1987,15(4):744-748
76.
Michael J. Gaskell Keith A. Jermyn Donald J. Watts Timothy Treffry Jeffrey G. Williams 《Differentiation; research in biological diversity》1992,51(3):171-176
Abstract. The ecm A and ecm B genes of Dictyostelium encode closely related extracellular matrix proteins. The major prestalk cell population, pstA cells, expresses the ecm A gene but not the ecm B gene. PstAB cells, a minor prestalk cell population that we show to express both the ecm A and ecm B genes, form a core in the centre of the slug tip. The rear, prespore region of the slug contains amoebae, termed anterior-like cells (ALC), that display many of the properties of prestalk cells. The ecm A and B genes are weakly expressed in about 30% of the ALC and these comprise a mixture of pstA cells, pstAB cells and a third class, pstB cells. The latter cell type express the ecm B gene but show no detectable expression of the ecm A gene. The demonstration of the existence of pstB cells suggests a separate pathway of ecm B gene induction, wherein expression of the ecm A gene is absent or at a very low level. Pst A, AB and B cells most probably differ in their surface properties because they are partially separable by Counter Current Distribution (CCD), a chromatographic technique which, in the conditions used, is dependent upon differences in cell surface hydrophobicity. 相似文献