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411.
A A Travers 《Biochemical Society transactions》1986,14(2):199-200
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The overall reaction catalyzed by the phosphotransferase arginine kinase was studied at normal and subzero temperatures. Ethylene glycol was used as the antifreeze and its effects on the Km values of substances, kcat and pH profiles were investigated in detail. a) The Km values for the substrate (2 mM for ATP and 0.6 mM for arginine) were little affected by the solvent composition or temperature of the reaction mixture. b) At concentration of ethylene glycol higher than 40% there was a sharp drop of enzyme activity. c) Ethylene glycol induces a large shift in the enzymic pK D) At -5 degrees C in 40% of solvent there was a break in the Arrhenius plot suggesting a change of the rate-limiting step. The relevance of these results to the reaction pathway of arginine kinase is discussed. In addition, controlled perturbations induced by cosolvent and temperature appear as useful tools for further kinetic investigations. 相似文献
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Activation thermodynamics of the binding of carbon monoxide to horseradish peroxidase. Role of pressure, temperature and solvent 总被引:1,自引:0,他引:1
The kinetics at 423 nm of the binding of carbon monoxide to ferrous horseradish peroxidase were studied as a function of three parameters: pressure (1-1200 bar), temperature (34 to -20 degrees C) and solvent (water, 40% ethylene glycol, 50% methanol) using a high-pressure stopped-flow apparatus. By using transition state theory the thermodynamic quantities delta V, delta S and delta H were determined under these different experimental conditions and were found to be greatly modulated by the physico-chemical parameters of the media. The results suggest that the macroscopic thermodynamic response is mainly controlled by the solvent. By adjusting two variables (among T, P, solvent), it is possible either to amplify or to cancel out the effect of the third. 相似文献
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The two step tight binding of ATP to myosin, heavy meromyosin and myosin subfragment 1 was investigated, under cryoenzymic conditions by the unlabeled ATP chase method: M + ATP in equilibrium K1 M.ATP k2 in equilibrium k-2 M*.ATP where M is myosin. k-2 is close to zero. In multi-turnover experiments, one obtains the constants for the binding process together with the concentration of ATPase sites. Here the kinetics of the formation of M*.ATP are first order. Inversion of the reagent concentrations (i.e., single-turnover experiments) should give identical kinetics but such experiments often give biphasic curves. This biphasicity depends upon the myosin preparation used and it is directly related to the active site titration. The simplest explanation for these results is one involving 2 sites for ATP: one site hydrolyzes ATP by the Bagshaw-Trentham scheme (tight binding preceding hydrolysis) but the second site binds ATP loosely without significant hydrolysis. This heterogeneity in ATP binding may explain certain difficulties, such as questions concerning the non-identity of the myosin heads and the number of steps involved in nucleotide binding. Attempts were made to determine the cause of the head heterogeneity but these were unsuccessful. We cannot exclude the possibility that the heterogeneity is relevant to muscle contraction. 相似文献
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M.T. Travers 《生物化学与生物物理学报:生物膜》2004,1661(1):106-112
The activity and expression of indoleamine 2,3-dioxygenase together with l-tryptophan transport has been examined in cultured human breast cancer cells. MDA-MB-231 but not MCF-7 cells expressed mRNA for indoleamine 2,3-dioxygenase. Kynurenine production by MDA-MB-231 cells, which was taken as a measure of enzyme activity, was markedly stimulated by interferon-γ (1000 units/ml). Accordingly, l-tryptophan utilization by MDA-MB-231 cells was enhanced by interferon-γ. 1-Methyl-dl-tryptophan (1 mM) inhibited interferon-γ induced kynurenine production by MBA-MB-231 cells. Kynurenine production by MCF-7 cells remained at basal levels when cultured in the presence of interferon-γ. l-Tryptophan transport into MDA-MB-231 cells was via a Na+-independent, BCH-sensitive pathway. It appears that system L (LAT1/CD98) may be the only pathway for l-tryptophan transport into these cells. 1-Methyl-d,l-tryptophan trans-stimulated l-tryptophan efflux from MDA-MB-231 cells and thus appears to be a transported substrate of system L. The results suggest that system L plays an important role in providing indoleamine-2,3-dioxygenase with its main substrate, l-tryptophan, and suggest a mechanism by which estrogen receptor-negative breast cancer cells may evade the attention of the immune system. 相似文献