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11.
12.
Francis R. Trainor 《Journal of phycology》1992,28(4):553-558
Scenedesmus armatus (Chod.) Chod, growth and morphology were monitored in medium 7 (oligotrophic) and Bristol's medium (eutrophic); cultures in both media were incubated at 10 and 22° C. Growth rate at 10° C was reduced, i.e. only one doubling in 7 days in medium 7 and 2.3 doublings in Bristol's, compared to 4.3 and 6 doublings at 22° C over the same period. Unicells as well as cells of colonies were larger at the cold temperature. The lengths of cells were not significantly different regardless of temperature or medium, but cell width was markedly increased at the lower temperature. Additionally, an arcuate, eight-celled, multispined ecomorph, which resembled several previously described taxa, was produced at 10° C. It becomes a component of a previously published ordered sequence of ecomorph development for this species. Based on data now accumulated in both the laboratory and the field, these temporal changes are interpreted to be a cyclomorphosis, driven by a coupling of nutrient availability and temperature. Within the addition of new cold temperature (spring) ecomorphs, the ordered sequence of ecomorphs for S. armatus is a succession from unicells to multispined eight-celled colonies to quadricaudate colonies, ending with acaudate four-celled ecomorphs. 相似文献
13.
Delayed DNA methylation is an integral feature of DNA replication in mammalian cells 总被引:2,自引:0,他引:2
D M Woodcock D L Simmons P J Crowther I A Cooper K J Trainor A A Morley 《Experimental cell research》1986,166(1):103-112
In the majority of sites of methylation in the DNA of mammalian cells, the symmetry of methylation is restored within a few minutes of the passage of a replication fork. However, it has been shown that daughter strand methylation in immortalised cell lines is delayed in a substantial minority of sites for up to several hours after replication. We report here the results of two new approaches to the determination of the functional significance of delayed DNA methylation in mammalian cells. Firstly, we demonstrate that normal, nontransformed cells (human peripheral lymphocytes in short-term primary culture) have comparable proportions of delayed DNA methylation to many immortalised cell lines, showing that delayed DNA methylation is not just a secondary consequence of abnormally high methionine requirements commonly observed in transformed cells and that delayed DNA methylation would be unlikely not to occur in vivo. Secondly, we have used 5-aza-2'-deoxycytidine (5azadCyd) to derive subclones of cells from the Chinese hamster ovary cell line which have stably hypomethylated DNA. In three of these subclones which had lost on average one fourth of the methylation sites from their genomes, the proportion of daughter strand methylation which was delayed after replication was reduced by less than 10%. If delayed DNA methylation were site-specific, this implies that of the order of twice the number of "immediate" methylation sites than delayed methylation sites had been lost from the genomes of these hypomethylated subclones. Thus, delayed DNA methylation is an integral part of the process whereby replicating mammalian cells maintain the pattern of methylation in their genomes. These observations are discussed in relation to the significance of delayed DNA methylation for the accurate maintenance of methylation patterns in the genome and the consequent implications for the possible role of methylated deoxycytidines in mammalian gene control. 相似文献
14.
Chris CR Smith Lisa K Snowberg J Gregory Caporaso Rob Knight Daniel I Bolnick 《The ISME journal》2015,9(11):2515-2526
To explain differences in gut microbial communities we must determine how processes regulating microbial community assembly (colonization, persistence) differ among hosts and affect microbiota composition. We surveyed the gut microbiota of threespine stickleback (Gasterosteus aculeatus) from 10 geographically clustered populations and sequenced environmental samples to track potential colonizing microbes and quantify the effects of host environment and genotype. Gut microbiota composition and diversity varied among populations. These among-population differences were associated with multiple covarying ecological variables: habitat type (lake, stream, estuary), lake geomorphology and food- (but not water-) associated microbiota. Fish genotype also covaried with gut microbiota composition; more genetically divergent populations exhibited more divergent gut microbiota. Our results suggest that population level differences in stickleback gut microbiota may depend more on internal sorting processes (host genotype) than on colonization processes (transient environmental effects). 相似文献
15.
16.
Cocuzza AJ Chidester DR Cordova BC Jeffrey S Parsons RL Bacheler LT Erickson-Viitanen S Trainor GL Ko SS 《Bioorganic & medicinal chemistry letters》2001,11(9):1177-1179
Two series of efavirenz analogues have been developed: one in which the cyclopropane ring has been replaced by small heterocycles and another in which the entire acetylenic side chain has been replaced by alkyloxy groups. Several members of both series show equivalent potency to efavirenz against both wild-type virus and the key K103N mutant. 相似文献
17.
Origins and plasticity of neural crest cells and their roles in jaw and craniofacial evolution 总被引:1,自引:0,他引:1
Trainor PA Melton KR Manzanares M 《The International journal of developmental biology》2003,47(7-8):541-553
The vertebrate head is a complex assemblage of cranial specializations, including the central and peripheral nervous systems, viscero- and neurocranium, musculature and connective tissue. The primary differences that exist between vertebrates and other chordates relate to their craniofacial organization. Therefore, evolution of the head is considered fundamental to the origins of vertebrates (Gans and Northcutt, 1983). The transition from invertebrate to vertebrate chordates was a multistep process, involving the formation and patterning of many new cell types and tissues. The evolution of early vertebrates, such as jawless fish, was accompanied by the emergence of a specialized set of cells, called neural crest cells which have long held a fascination for developmental and evolutionary biologists due to their considerable influence on the complex development of the vertebrate head. Although it has been classically thought that protochordates lacked neural crest counterparts, the recent identification and characterization of amphioxus and ascidian genes homologous to those involved in vertebrate neural crest development challenges this idea. Instead it suggests thatthe neural crest may not be a novel vertebrate cell population, but could have in fact originated from the protochordate dorsal midline epidermis. Consequently, the evolution of the neural crest cells could be reconsidered in terms of the acquisition of new cell properties such as delamination-migration and also multipotency which were key innovations that contributed to craniofacial development. In this review we discuss recent findings concerning the inductive origins of neural crest cells, as well as new insights into the mechanisms patterning this cell population and the subsequent influence this has had on craniofacial evolution. 相似文献
18.
The neural crest is a fascinating population of cells that migrate long distances in the developing embryo to generate many different derivatives. It also occupies a central position in the origin and patterning of the vertebrate head, and has generated debates about issues such as cell programming versus plasticity and the role of cell death in early morphogenesis. These aspects of the field were revisited and discussed in a recent meeting organized to honour the retirement of Jim Weston and his contribution to the field. 相似文献
19.
Mouse embryos with a loss-of-function mutation in the gene encoding the receptor tyrosine kinase ErbB4 exhibit misprojections of cranial sensory ganglion afferent axons. Here we analyse ErbB4-deficient mice, and find that morphological differences between wild-type and mutant cranial ganglia correlate with aberrant migration of a subpopulation of hindbrain-derived cranial neural crest cells within the paraxial mesenchyme environment. In transplantation experiments using new grafting techniques in cultured mouse embryos, we determine that this phenotype is non-cell-autonomous: wild-type and mutant neural crest cells both migrate in a pattern consistent with the host environment, deviating from their normal pathway only when transplanted into mutant embryos. ErbB4 signalling events within the hindbrain therefore provide patterning information to cranial paraxial mesenchyme that is essential for the proper migration of neural crest cells. 相似文献
20.
A single amino acid substitution in v-erbB confers a thermolabile phenotype to ts167 avian erythroblastosis virus-transformed erythroid cells. 总被引:13,自引:5,他引:13 下载免费PDF全文
A library of recombinant bacteriophage was prepared from ts167 avian erythroblastosis virus-transformed erythroid precursor cells (HD6), and integrated proviruses from three distinct genomic loci were isolated. A subclone of one of these proviruses (pAEV1) was shown to confer temperature-sensitive release from transformation of erythroid precursor cells in vitro. The predicted amino acid sequence of the v-erbB polypeptide from the mutant had a single amino acid change when compared with the wild-type parental virus. When the wild-type amino acid was introduced into the temperature-sensitive avian erythroblastosis virus provirus in pAEV1, all erythroid clones produced in vitro were phenotypically wild type. The mutation is a change from a histidine to an aspartic acid in the temperature-sensitive v-erbB polypeptide. It is located in the center of the tyrosine-specific protein kinase domain and corresponds to amino acid position 826 of the human epidermal growth factor receptor sequence. 相似文献