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71.
Tadashi Matsunaga Isao Karube Shuichi Suzuki 《Applied microbiology and biotechnology》1980,10(3):235-243
Summary A microbial sensor consisting of immobilized Clostridium butyricum, two gas permeable Teflon membranes and fuel cell type electrode was suitable for the determination of formic acid. When the sensor was inserted into the sample solution containing formic acid, the current increases to a steady state with a response time of 20 min. The relationship between the steady state current and the formic acid concentration is linear up to 1 000 mg l–1. The currents are reproducible with an average relative error of 5%. Selectivity of the sensor is satisfactory. Results obtained with this sensor and by gas chromatography were in good agreement (regression coefficient; 0.98) when the cultivation medium of Aeromonas formicans was employed. Immobilized Clostridium butyricum is stable for more than 20 days. 相似文献
72.
Conditions are described under which crystals are formed with equimolar complex of mitochondrial cytochrome oxidase and cytochrome c. Characteristic absorption bands of the solubilized crystals could be attributed to the cytochrome oxidase-cytochrome c complex with heme a:c ratio of 2:1. Activity of crystals shows more close heme-heme interaction between two cytochromes than that of the mixture. 相似文献
73.
Cytochrome oxidase of Nitrobacter agilis: isolation by hydrophobic interaction chromatography 总被引:1,自引:0,他引:1
Cytochrome oxidase has been purified from Nitrobacter agilis using hydrophobic interaction chromatography. The purified preparation contained 3-5% phospholipid and migrated as a single band during polyacrylamide gel electrophoresis under nondissociating conditions, but appeared as three bands in the presence of sodium dodecyl sulfate and 6 M urea. These three bands corresponded to molecular weights of 37 000, 25 000, and 13 000. The absorption spectra of cytochrome oxidase isolated from Nitrobacter were similar to those reported for a-type cytochrome oxidase from other sources and exhibited absorption maxima at 420 and 600 nm when oxidized and 443 and 606 nm when reduced. The purified enzyme reacted both with horse heart and Nitrobacter cytochrome c. The enzymatic activity depended upon the pH of reaction mixture, with the maximum activity at pH 6.5 and 7.5 for Nitrobacter and horse heart cytochrome c, respectively. The activity of the purified enzyme was inhibited by cyanide, azide, and diethyl dithiocarbamate. 相似文献
74.
1,4-Methylhistamine was characterized as substrate for monoamine oxidase (MAO) in rat liver mitochondria. The m and max values were 38.8 μM and 6.33 nmoles/mg protein/60 min, respectively. The inhibition experiments with clorgyline and deprenyl, the selective inhibitors for type A and type B MAO, showed that 1,4-methylhistamine was specific for type B MAO. 相似文献
75.
76.
77.
From the results of 13C-nmr measurement of poly(β-benzyl-L -aspartate) and its model compounds in dimethyl sulphoxide/deuterated chloroform mixtures, it was found that the side chain of poly(β-benzyl-L -aspartate) is solvated by dimethyl sulphoxide in the region more than dimethyl sulphoxide 20% (v/v), where the backbone maintains the α-helix. The chemical shift differences in the benzyl group carbons of poly(γ-benzyl-L -glutamate) (trifluoroacetic acid/deuterated chloroform) accompanied by the helix-coil transition, originate from the interaction between the ester group of the side chain and trifluoroacetic acid. The chemical shift difference in the ester carbon is similar. On the other hand, the chemical shift differences of the side-chain carbons in the alkyl portion (Cβ, Cγ) originate not only from the interaction between the ester group of the side chain and trifluoroacetic acid, but also from some other unknown factors. The chemical shift differences of the side-chain carbons of poly(β-benzyl-L -aspartate) originate from the interaction between the ester group of the side chain and trifluoroacetic acid. 相似文献
78.
Aminoacylase was immobilized on the mycelium pellets of Aspergillus ochraceus by using albumin and glutaraldehyde. No difference in the optimum pH was observed between native aminoacylase and aminoacylase pellets. The aminoacylase pellets were stable in pH 4-8 but they were unstable in alkaline conditions. The aminoacylase pellets were more stable against heavy metal ions and inhibitors than native aminoacylase. However, the degree of the activation of aminoacylase with cobalt ion decreased with the immobilization. It was suggested that most of aminoacylase was covalently coupled to the mycelium with glutaraldehyde. 相似文献
79.
Dye-coupled electrode system for the rapid determination of cell populations in polluted water. 总被引:7,自引:1,他引:6 下载免费PDF全文
We determined cell populations in polluted waters by using a fuel cell-type electrode. The electrode was constructed from a platinum anode, a silver peroxide cathode, and a membrane filter for retaining microorganisms. The principle of cell number determination is based on sensing a redox dye reduced by the microorganisms with the electrode. Sample solutions containing microorganisms were membrane filtered, and the resulting filter containing microbial cells was attached to the surface of a platinum anode. The electrode was immersed in phosphate buffer solution (0.05 M, pH 7) containing a redox dye (2,4-dichlorophenol-indophenol), and the current generated was measured. The response time of the electrode system was 10 to 20 min, and the current generated was proportional to cell populations above 10(4) cells/ml. 相似文献
80.
Osamu Suzuki Hideki Hattori Makoto Sawada Toshiharu Nagatsu Naomasa Miki Haruhiro Higashida 《Neurochemistry international》1983,5(5):599-601
Mouse neuroblastoma × rat glioma NG108-15 hybrid cells contain a considerable amount of serotonin, and possess small but significant tryptophan hydroxylase activity. The results suggest that NG108-15 hybrid cells are serotonergic, in addition to the known cholinergic property. 相似文献