全文获取类型
收费全文 | 180篇 |
免费 | 20篇 |
国内免费 | 1篇 |
出版年
2021年 | 3篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2017年 | 5篇 |
2016年 | 8篇 |
2015年 | 5篇 |
2014年 | 4篇 |
2013年 | 7篇 |
2012年 | 7篇 |
2011年 | 9篇 |
2010年 | 15篇 |
2009年 | 10篇 |
2008年 | 11篇 |
2007年 | 11篇 |
2006年 | 6篇 |
2005年 | 2篇 |
2004年 | 8篇 |
2003年 | 10篇 |
2002年 | 4篇 |
2001年 | 8篇 |
2000年 | 5篇 |
1999年 | 4篇 |
1998年 | 11篇 |
1997年 | 7篇 |
1996年 | 6篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 6篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1988年 | 3篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1979年 | 1篇 |
1978年 | 3篇 |
1977年 | 1篇 |
1975年 | 2篇 |
1942年 | 1篇 |
排序方式: 共有201条查询结果,搜索用时 31 毫秒
111.
112.
Eric Scott Sills MJ Burns Laurinda D Parker Lisa P Carroll Lisa L Kephart CS Dyer Peter R Papenhausen Jessica G Davis 《Orphanet journal of rare diseases》2007,2(1):1-5
Hypoplastic left heart syndrome(HLHS) refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis. HLHS has been reported to occur in approximately 0.016 to 0.036% of all live births. Newborn infants with the condition generally are born at full term and initially appear healthy. As the arterial duct closes, the systemic perfusion becomes decreased, resulting in hypoxemia, acidosis, and shock. Usually, no heart murmur, or a non-specific heart murmur, may be detected. The second heart sound is loud and single because of aortic atresia. Often the liver is enlarged secondary to congestive heart failure. The embryologic cause of the disease, as in the case of most congenital cardiac defects, is not fully known. The most useful diagnostic modality is the echocardiogram. The syndrome can be diagnosed by fetal echocardiography between 18 and 22 weeks of gestation. Differential diagnosis includes other left-sided obstructive lesions where the systemic circulation is dependent on ductal flow (critical aortic stenosis, coarctation of the aorta, interrupted aortic arch). Children with the syndrome require surgery as neonates, as they have duct-dependent systemic circulation. Currently, there are two major modalities, primary cardiac transplantation or a series of staged functionally univentricular palliations. The treatment chosen is dependent on the preference of the institution, its experience, and also preference. Although survival following initial surgical intervention has improved significantly over the last 20 years, significant mortality and morbidity are present for both surgical strategies. As a result pediatric cardiologists continue to be challenged by discussions with families regarding initial decision relative to treatment, and long-term prognosis as information on long-term survival and quality of life for those born with the syndrome is limited. 相似文献
113.
114.
The potent antimalarial sesquiterpene lactone, artemisinin, is produced in low quantities by the plant Artemisia annua L. The source and regulation of the isopentenyl diphosphate (IPP) used in the biosynthesis of artemisinin has not been completely
characterized. Terpenoid biosynthesis occurs in plants via two IPP-generating pathways: the mevalonate pathway in the cytosol,
and the non-mevalonate pathway in plastids. Using inhibitors specific to each pathway, it is possible to resolve which supplies
the IPP precursor to the end product. Here, we show the effects of inhibition on the two pathways leading to IPP for artemisinin
production in plants. We grew young (7–14 days post cotyledon) plants in liquid culture, and added mevinolin to the medium
to inhibit the mevalonate pathway, or fosmidomycin to inhibit the non-mevalonate pathway. Artemisinin levels were measured
after 7–14 days incubation, and production was significantly reduced by each inhibitor compared to controls, thus, it appears
that IPP from both pathways is used in artemisinin production. Also when grown in miconazole, an inhibitor of sterol biosynthesis,
there was a significant increase in artemisinin compared to controls suggesting that carbon was shifted from sterols into
sesquiterpenes. Collectively these results indicate that artemisinin is probably biosynthesized from IPP pools from both the
plastid and the cytosol, and that carbon from competing pathways can be channeled toward sesquiterpenes. This information
will help advance our understanding of the regulation of in planta production of artemisinin. 相似文献
115.
Gruessner B. M. Cornet-Vernet L. Desrosiers M. R. Lutgen P. Towler M. J. Weathers P. J. 《Phytochemistry Reviews》2019,18(6):1509-1527
Phytochemistry Reviews - Artemisia sp., especially A. annua and A. afra, have been used for centuries to treat many ailments. While artemisinin is the main therapeutically active component,... 相似文献
116.
117.
Conditional inactivation of FGF receptor 2 reveals an essential role for FGF signaling in the regulation of osteoblast function and bone growth 总被引:19,自引:0,他引:19
Yu K Xu J Liu Z Sosic D Shao J Olson EN Towler DA Ornitz DM 《Development (Cambridge, England)》2003,130(13):3063-3074
Human craniosynostosis syndromes, resulting from activating or neomorphic mutations in fibroblast growth factor receptor 2 (FGFR2), underscore an essential role for FGFR2 signaling in skeletal development. Embryos harboring homozygous null mutations in FGFR2 die prior to skeletogenesis. To address the role of FGFR2 in normal bone development, a conditional gene deletion approach was adopted. Homologous introduction of cre recombinase into the Dermo1 (Twist2) gene locus resulted in robust expression of CRE in mesenchymal condensations giving rise to both osteoblast and chondrocyte lineages. Inactivation of a floxed Fgfr2 allele with Dermo1-cre resulted in mice with skeletal dwarfism and decreased bone density. Although differentiation of the osteoblast lineage was not disturbed, the proliferation of osteoprogenitors and the anabolic function of mature osteoblasts were severely affected. 相似文献
118.
In vitro zygotic embryo culture of wild banana significantly increased the germination compared to greenhouse grown seeds. Embryo orientation and BAP concentration significantly affected germination rate. These factors together with gelling agent, dark and light conditions and coconut water, also showed variable effects on the number of roots per plant, root length, shoot length, number of days to root emergence and number of days to shoot emergence. 相似文献
119.
Diaz-Perez, S. V., Crouch, V. W., and Orbach, M. J. 1996. Construction and characterization of a Magnaporthe grisea bacterial artificial chromosome library. Fungal Genet. Biol. 20, 280-288. A bacterial artificial chromosome (BAC) library of Magnaporthe grisea containing 4128 clones with an average insert size of 66-kb has been constructed. This library represents seven genome equivalents of M. grisea and has been demonstrated to be representative of the genome by screening for the presence of several single-copy genes and DNA markers. The utility of the library for use in map-based cloning projects was shown by the spanning of a nine-cosmid, 207-kb DNA contig with only 3 BAC clones. In addition, using a lys1-3 auxotroph, we have shown that BAC clones at least 113 kb can be transformed into M. grisea to screen for complementation of mutations. Thus, BACs isolated in chromosome walks can be rapidly screened for the presence of the sought after gene. The ease of construction of BAC libraries and of isolation and manipulation of BAC clones makes the BAC system an ideal one for physical analyses of fungal genomes. 相似文献
120.
D.M. J.M. Lucocq M.C. Towler S. Clough J. James S.R. James C.P. Downes S. Ponnambalam 《Current biology : CB》1997,7(12)
The eukaryotic trans-Golgi network (TGN) is a key site for the formation of transport vesicles destined for different intracellular compartments [1]. A key marker for the mammalian TGN is TGN38/46 [2]. This integral membrane glycoprotein cycles between the TGN and the cell surface and is implicated in recruitment of cytosolic factors and regulation of at least one type of vesicle formation at the mammalian TGN [2] and [3]. In this study, we have identified a phosphatidylinositol (PtdIns)-specific 3-kinase activity associated with the human orthologue (TGN46), which is sensitive to lipid kinase inhibitors. Treatment of HeLa cells with low levels of these inhibitors reveals subtle morphological changes in TGN46-positive compartments. Our findings suggest a role for PtdIns 3-kinases and presumably for the product, PtdIns 3-phosphate (PtdIns3P), in the formation of secretory transport vesicles by mechanisms conserved in yeast and mammals. 相似文献