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11.
Shizuya Tanaka Toshiro Kato Shigeo Yamamoto Hirosuke Yoshioka 《Bioscience, biotechnology, and biochemistry》2013,77(10):1953-1959
Since S-n-butyl S′-p-tert-butylbenzyl N-3-pyridyldithiocarbonimidate, a potent fungicide to powdery mildew, is known to inhibit ergosterol biosynthesis in Monilinia fructigena, the activities were assessed on 24 compounds having other substituents than the 3-pyridyl and on 24 compounds having a variety of different structures connecting the 3-pyridyl and the p-tert-butylphenyl group from that of the dithiocarbonimidate.In the former group the 3-pyridyl group was essential for the activities and the substitution at the 2- or 6-position resulted, on available data, in inactive compounds. Several other β-N-heterocyclic analogs were also active. In the latter group, a number of modified compounds from the dithiocarbonimidate structure were shown to be active. 相似文献
12.
Masaaki Umeda Momoko Ikeuchi Masaki Ishikawa Toshiro Ito Ryuichi Nishihama Junko Kyozuka Keiko U. Torii Akiko Satake Gohta Goshima Hitoshi Sakakibara 《The Plant journal : for cell and molecular biology》2021,106(2):326-335
Plant stem cells have several extraordinary features: they are generated de novo during development and regeneration, maintain their pluripotency, and produce another stem cell niche in an orderly manner. This enables plants to survive for an extended period and to continuously make new organs, representing a clear difference in their developmental program from animals. To uncover regulatory principles governing plant stem cell characteristics, our research project ‘Principles of pluripotent stem cells underlying plant vitality’ was launched in 2017, supported by a Grant-in-Aid for Scientific Research on Innovative Areas from the Japanese government. Through a collaboration involving 28 research groups, we aim to identify key factors that trigger epigenetic reprogramming and global changes in gene networks, and thereby contribute to stem cell generation. Pluripotent stem cells in the shoot apical meristem are controlled by cytokinin and auxin, which also play a crucial role in terminating stem cell activity in the floral meristem; therefore, we are focusing on biosynthesis, metabolism, transport, perception, and signaling of these hormones. Besides, we are uncovering the mechanisms of asymmetric cell division and of stem cell death and replenishment under DNA stress, which will illuminate plant-specific features in preserving stemness. Our technology support groups expand single-cell omics to describe stem cell behavior in a spatiotemporal context, and provide correlative light and electron microscopic technology to enable live imaging of cell and subcellular dynamics at high spatiotemporal resolution. In this perspective, we discuss future directions of our ongoing projects and related research fields. 相似文献
13.
Osamu Takase Masahiro Yoshikawa Mana Idei Junichi Hirahashi Toshiro Fujita Tsuyoshi Takato Takayuki Isagawa Genta Nagae Hirofumi Suemori Hiroyuki Aburatani Keiichi Hishikawa 《PloS one》2013,8(2)
NF-κB signaling plays an essential role in maintaining the undifferentiated state of embryonic stem (ES) cells. However, opposing roles of NF-κB have been reported in mouse and human ES cells, and the role of NF-κB in human induced pluripotent stem (iPS) cells has not yet been clarified. Here, we report the role of NF-κB signaling in maintaining the undifferentiated state of human iPS cells. Compared with differentiated cells, undifferentiated human iPS cells showed an augmentation of NF-κB activity. During differentiation induced by the removal of feeder cells and FGF2, we observed a reduction in NF-κB activity, the expression of the undifferentiation markers Oct3/4 and Nanog, and the up-regulation of the differentiated markers WT-1 and Pax-2. The specific knockdown of NF-κB signaling using p65 siRNA also reduced the expression of Oct3/4 and Nanog and up-regulated WT-1 and Pax-2 but did not change the ES-like colony formation. Our results show that the augmentation of NF-κB signaling maintains the undifferentiated state of human iPS and suggest the importance of this signaling pathway in maintenance of human iPS cells. 相似文献
14.
Humio Kurasawa Yoshiaki Kanauti Koji Takei Shiroshi Ogawa Tetsutaro Okabe Toshiro Hayakawa 《Bioscience, biotechnology, and biochemistry》2013,77(10):1809-1813
A screening was designed to isolate microorganisms having poly(γ-glutamic acid) (PGA) endohydrolase activity. Of the strains screened, TM-4222, from a soil sample, showed the highest viscosity decrement ability on PGA. It was identified to be a Myrothecium sp. The fungal production of the enzyme was slightly promoted with yeast extract and greatly promoted with· both yeast extract and PGA. The fungus was evaluated to produce PGA hydrolase of an endo-type specificity by analyzing of the reaction products. 相似文献
15.
Kiyoshi Yoshizawa Toshiro Furukawa Makoto Tadenuma Masakazu Yamada 《Bioscience, biotechnology, and biochemistry》2013,77(4):326-332
We have found that some straight-chained α-amino acids are converted by yeast to the alcohols with correspondingly longer carbon chains in the alcoholic fermentation contrary to F. Ehrlich’s scheme, i.e., isobutyl alcohol from alanine and active amyl alcohol from α-amino-n-butyric acid or threonine.In this report, we confirmed this fact in the alcoholic fermentation of many aliphatic amino acids by 2 yeast strains using gas chromatography. Moreover, n-propyl alcohol was proved to come from α-amino-n-butyric acid or threonine. Small quantities of n-propyl, isobutyl, active amyl and isoamyl alcohols were found in all the fermented solutions. There was some difference in the composition of higher alcohols of the alcoholic solutions fermented by different yeasts. 相似文献
16.
Humio Kurasawa Yoshiaki Kanauchi Tadakatsu Wakayama Toshiro Hayakawa Ikuo Igaue 《Bioscience, biotechnology, and biochemistry》2013,77(12):2913-2916
Varoius piericidin analogues (PS-I, -II and -III in Fig. 2) were synthesized from three 4-acetoxy-6-formylpyridines by Wittig reaction to determine the structure-activity relationships. New type inhibitors, 5-alkenyl-2, 3-dimethoxy-4-hydroxy-6-methylpyridines (PS-IV) were synthesized by intramolecular cyclization. 相似文献
17.
Shizuya Tanaka Toshiro Kato Shigeo Yamamoto Hirosuke Yoshioka 《Bioscience, biotechnology, and biochemistry》2013,77(9):1627-1633
Fungicidal activities of S-alkyl S′-p-substituted benzyl N-3-pyridyldithiocarbonimidates toward Coniothyrium diplodiella, Scleotinia sclerotiorum (on agar medium) and Sphaerotheca fuliginea (on pot test) were shown. The activities varied with the change of the S-alkyl group and were maximized at certain numbers of carbon atoms. A different pattern was observed in the activity toward S. fuliginea when the carbon number was 8 or more. Bulkiness of the S-alkyl group also appeared to influence the activities. The steric factor of the p-alkyl substituents mainly influenced the activity toward S. fuliginea up to the tert-butyl analog. The activities toward C. diplodiella and S. sclerotiorum increased with the increase in the bulkiness and the hydrophylicity of the p-substituent. Rm values were determined on reversed phase TLC and the structure-activity correlations were analyzed against hydrophobic (Rm), electronic (σ) and steric (Es) factors on 34 compounds. 相似文献
18.
19.
Toshiro Samejima Yoshihiko Soh Toshimasa Yano 《Bioscience, biotechnology, and biochemistry》2013,77(12):2285-2290
The equilibrium moisture contents of sun-cured (Kroumougrad), flue-cured (Bright Yellow—4) and air-cured (Burley-21 and Matsukawa) tobaccos were measured over a relative humidity range from 5 to 80% at 20°C. The moisture sorption isotherms of tobaccos were of sigmoid type, and classified into two groups. In a lower humidity range below ca. 40% RH, the A group (Kroumougrad and BY-4) had a smaller moisture sorption capacity than B group (Burley-21 and Matsukawa), while in a higher humidity range above ca. 50% RH the former had a larger moisture sorption capacity than the latter. By extracting with water, the moisture content of BY-4 was increased in the lower humidity range, while it decreased in the higher humidity range. However, the moisture content of Matsukawa was scarecely changed by extracting it with water. These results suggest that the differences in equilibrium moisture content with the type of curing were due to the differences in contents of water soluble com- ponents. To control the hygroscopic properties of a tobacco, therefore, the influences of the addition of sucrose and glycerol on the equilibrium moisture content were quantitatively analysed. The moisture sorption capacity of tobacco was greatly different from its nitrogen sorption capacity. The specific surface area of tobacco calculated from moisture sorption isotherm was ca. 110 times larger than the specific surface area calculated from the nitrogen sorption isotherm. Both the nitrogen and moisture sorption data should be necessary for better understanding of the complicated sorption-desorption phenomena in tobaccos. 相似文献
20.
The reversible interaction between dextran sulfate (D) and the low density lipoprotein of human serum (P) was investigated by sedimentation velocity. Analysis of the velocity patterns of dextran sulfate—lipoprotein mixtures revealed that the maximum number of binding sites on dextran sulfate molecule is approximately 6. It was also shown that the species of the complex formed is affected by the mixing ratio of the two constituents: at the molar ratio (P/D) 0.69, the complex exists in average as DP1.6 and at 0.98 as DP2.2. The linear increment of sedimentation coefficient of the complex due to the binding of one lipoprotein molecule was 7.8S. Finally, the mechanism of precipitation of the complexes was discussed. 相似文献