全文获取类型
收费全文 | 2985篇 |
免费 | 139篇 |
国内免费 | 3篇 |
出版年
2022年 | 8篇 |
2021年 | 21篇 |
2019年 | 17篇 |
2018年 | 24篇 |
2017年 | 21篇 |
2016年 | 31篇 |
2015年 | 50篇 |
2014年 | 73篇 |
2013年 | 253篇 |
2012年 | 158篇 |
2011年 | 152篇 |
2010年 | 92篇 |
2009年 | 87篇 |
2008年 | 161篇 |
2007年 | 165篇 |
2006年 | 172篇 |
2005年 | 183篇 |
2004年 | 187篇 |
2003年 | 208篇 |
2002年 | 194篇 |
2001年 | 30篇 |
2000年 | 16篇 |
1999年 | 28篇 |
1998年 | 51篇 |
1997年 | 50篇 |
1996年 | 35篇 |
1995年 | 34篇 |
1994年 | 38篇 |
1993年 | 28篇 |
1992年 | 43篇 |
1991年 | 28篇 |
1990年 | 22篇 |
1989年 | 27篇 |
1988年 | 31篇 |
1987年 | 24篇 |
1986年 | 13篇 |
1985年 | 23篇 |
1984年 | 33篇 |
1983年 | 34篇 |
1982年 | 42篇 |
1981年 | 36篇 |
1980年 | 27篇 |
1979年 | 17篇 |
1978年 | 17篇 |
1977年 | 18篇 |
1976年 | 15篇 |
1975年 | 19篇 |
1974年 | 14篇 |
1973年 | 9篇 |
1968年 | 7篇 |
排序方式: 共有3127条查询结果,搜索用时 515 毫秒
141.
Recent advances in soil seed bank research 总被引:1,自引:0,他引:1
Yongcui Wang Deming Jiang Oshida Toshio Quanlai Zhou 《Contemporary Problems of Ecology》2013,6(5):520-524
Soil seed bank is an important component of ecosystem resilience and represents a stock of regeneration potential in many plant assemblages. Soil seed banks can be used to predict the composition of new plant recruitment. Species overcome periods of unfavorable weather conditions by building up a large seed bank. With this strategy, the species diversity is preserved and information on their dynamics and structure is retained. The research on soil seed bank has received wide interest because of its important role in plantation renovation and restoration, biological diversity preservation, vegetation succession and diffusion processes and other aspects. This paper reviews the relationship between soil seed bank and disturbance, geographic factor, vegetation and seed size and is aimed at providing some useful reference for future research. 相似文献
142.
Kohei Fujikura Tomiyoshi Setsu Kenji Tanigaki Takaya Abe Hiroshi Kiyonari Toshio Terashima Toshiaki Sakisaka 《PloS one》2013,8(1)
We describe a novel spontaneous mouse mutant, laggard (lag), characterized by a flat head, motor impairment and growth retardation. The mutation is inherited as an autosomal recessive trait, and lag/lag mice suffer from cerebellar ataxia and die before weaning. lag/lag mice exhibit a dramatic reduction in brain size and slender optic nerves. By positional cloning, we identify a splice site mutation in Kif14. Transgenic complementation with wild-type Kif14-cDNA alleviates ataxic phenotype in lag/lag mice. To further confirm that the causative gene is Kif14, we generate Kif14 knockout mice and find that all of the phenotypes of Kif14 knockout mice are similar to those of lag/lag mice. The main morphological abnormality of lag/lag mouse is severe hypomyelination in central nervous system. The lag/lag mice express an array of myelin-related genes at significantly reduced levels. The disrupted cytoarchitecture of the cerebellar and cerebral cortices appears to result from apoptotic cell death. Thus, we conclude that Kif14 is essential for the generation and maturation of late-developing structures such as the myelin sheath, cerebellar and cerebral cortices. So far, no Kif14-deficient mice or mutation in Kif14 has ever been reported and we firstly define the biological function of Kif14 in vivo. The discovery of mammalian models, laggard, has opened up horizons for researchers to add more knowledge regarding the etiology and pathology of brain malformation. 相似文献
143.
144.
145.
You Li Hisato Yagi Ezenwa Obi Onuoha Rama Rao Damerla Richard Francis Yoshiyuki Furutani Muhammad Tariq Stephen M. King Gregory Hendricks Cheng Cui Manush Saydmohammed Dong Min Lee Maliha Zahid Iman Sami Linda Leatherbury Gregory J. Pazour Stephanie M. Ware Toshio Nakanishi Elizabeth Goldmuntz Michael Tsang Cecilia W. Lo 《PLoS genetics》2016,12(2)
Heterotaxy, a birth defect involving left-right patterning defects, and primary ciliary dyskinesia (PCD), a sinopulmonary disease with dyskinetic/immotile cilia in the airway are seemingly disparate diseases. However, they have an overlapping genetic etiology involving mutations in cilia genes, a reflection of the common requirement for motile cilia in left-right patterning and airway clearance. While PCD is a monogenic recessive disorder, heterotaxy has a more complex, largely non-monogenic etiology. In this study, we show mutations in the novel dynein gene DNAH6 can cause heterotaxy and ciliary dysfunction similar to PCD. We provide the first evidence that trans-heterozygous interactions between DNAH6 and other PCD genes potentially can cause heterotaxy. DNAH6 was initially identified as a candidate heterotaxy/PCD gene by filtering exome-sequencing data from 25 heterotaxy patients stratified by whether they have airway motile cilia defects. dnah6 morpholino knockdown in zebrafish disrupted motile cilia in Kupffer’s vesicle required for left-right patterning and caused heterotaxy with abnormal cardiac/gut looping. Similarly DNAH6 shRNA knockdown disrupted motile cilia in human and mouse respiratory epithelia. Notably a heterotaxy patient harboring heterozygous DNAH6 mutation was identified to also carry a rare heterozygous PCD-causing DNAI1 mutation, suggesting a DNAH6/DNAI1 trans-heterozygous interaction. Furthermore, sequencing of 149 additional heterotaxy patients showed 5 of 6 patients with heterozygous DNAH6 mutations also had heterozygous mutations in DNAH5 or other PCD genes. We functionally assayed for DNAH6/DNAH5 and DNAH6/DNAI1 trans-heterozygous interactions using subthreshold double-morpholino knockdown in zebrafish and showed this caused heterotaxy. Similarly, subthreshold siRNA knockdown of Dnah6 in heterozygous Dnah5 or Dnai1 mutant mouse respiratory epithelia disrupted motile cilia function. Together, these findings support an oligogenic disease model with broad relevance for further interrogating the genetic etiology of human ciliopathies. 相似文献
146.
Assay-guided fractionation of the methanol extract from black seeds of sesame (Sesamum indicum L.) led to the isolation of an active compound that had a 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. This antioxidant was confirmed to be anthrasesamone F, an anthraquinone derivative previously isolated from different black sesame seeds and biogenetically related to other anthrasesamones in sesame roots. The radical scavenging assay showed that anthrasesamone F had more potent activity than Trolox. The content of anthrasesamone F in different parts and at different developmental stages of black sesame seeds was investigated to clarify the accumulation pattern of this antioxidant in the black seeds. Anthrasesamone F was localized in the seed coat of black seeds and accumulated after the seed coat color changed to black. The content of anthrasesamone F increased gradually with seed maturation and drastically on air-drying, the final stage in sesame cultivation. 相似文献
147.
148.
Takashi Yazawa Yoshitaka Imamichi Koh‐ichi Yuhki Junsuke Uwada Daisuke Mikami Masayuki Shimada Kaoru Miyamoto Takeshi Kitano Satoru Takahashi Toshio Sekiguchi Nobuo Suzuki Md. Rafiqul Islam Khan Fumitaka Ushikubi Akihiro Umezawa Takanobu Taniguchi 《Molecular reproduction and development》2019,86(7):786-797
149.
Yuichiro Maeda Toshio Hasegawa Etsuko Komiyama Yusuke Hirasawa Hitoshi Tsuchihashi Takasuke Ogawa Jonghun Kim Seiichiro Ando Akio Nagasaka Naoto Miura Shigaku Ikeda 《Journal of biophotonics》2019,12(4)
In finger vein authentication technology, near‐infrared rays penetrate the finger and are absorbed by the hemoglobin in blood. The veins appear as dark areas. The finger vein pattern images of patients with various diseases were acquired; a new evaluation method applying image processing technique (“E value”) was developed, and it was examined whether the patterns have any characteristics differentiating them from those of healthy volunteers. As a result, low E values appeared in systemic sclerosis, mixed connective tissue disease, Sjögren's syndrome, and polymyositis/dermatomyositis. No statistical reduction in E value was shown in patients with rheumatoid arthritis, pernio (without rheumatic diseases), arteriosclerosis obliterans, diabetes, hypertension, hypothyroidism and alopecia areata. This technology could be used for screening and evaluation of some diseases and their conditions with impaired peripheral venous circulation. E value may be useful as an indicator of venous circulation. 相似文献
150.