全文获取类型
收费全文 | 10098篇 |
免费 | 567篇 |
专业分类
10665篇 |
出版年
2022年 | 59篇 |
2021年 | 108篇 |
2020年 | 59篇 |
2019年 | 86篇 |
2018年 | 116篇 |
2017年 | 111篇 |
2016年 | 187篇 |
2015年 | 280篇 |
2014年 | 305篇 |
2013年 | 578篇 |
2012年 | 491篇 |
2011年 | 511篇 |
2010年 | 294篇 |
2009年 | 315篇 |
2008年 | 467篇 |
2007年 | 466篇 |
2006年 | 407篇 |
2005年 | 449篇 |
2004年 | 431篇 |
2003年 | 429篇 |
2002年 | 418篇 |
2001年 | 357篇 |
2000年 | 383篇 |
1999年 | 278篇 |
1998年 | 127篇 |
1997年 | 96篇 |
1996年 | 70篇 |
1995年 | 85篇 |
1994年 | 75篇 |
1993年 | 91篇 |
1992年 | 201篇 |
1991年 | 201篇 |
1990年 | 203篇 |
1989年 | 192篇 |
1988年 | 176篇 |
1987年 | 146篇 |
1986年 | 120篇 |
1985年 | 112篇 |
1984年 | 111篇 |
1983年 | 90篇 |
1982年 | 78篇 |
1981年 | 67篇 |
1979年 | 88篇 |
1978年 | 66篇 |
1977年 | 64篇 |
1976年 | 58篇 |
1975年 | 61篇 |
1974年 | 58篇 |
1973年 | 55篇 |
1972年 | 63篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
991.
1. Body size is often an important character in mating success, but has been only infrequently mentioned in regard to colour polymorphism. In this study, mating success was investigated in a colour polymorphic Ladybird Beetle, Harmonia axyridis , with reference both to colour morph and to body size.
2. In the non-melanic males the mating individuals were significantly larger than solitary individuals, while in melanic males there was no significant difference.
3. The mating pattern was close to random mating with respect to colour morph and there was no significant deviation.
4. The results suggest both body size and colour morph affect the male mating success and males of different body size obtain mating advantage according to the colour morph. Colour polymorphism in this species is controlled by alleles on a single locus. Thus, the alleles on that locus significantly influence the effect of selection on the quantitative character. 相似文献
2. In the non-melanic males the mating individuals were significantly larger than solitary individuals, while in melanic males there was no significant difference.
3. The mating pattern was close to random mating with respect to colour morph and there was no significant deviation.
4. The results suggest both body size and colour morph affect the male mating success and males of different body size obtain mating advantage according to the colour morph. Colour polymorphism in this species is controlled by alleles on a single locus. Thus, the alleles on that locus significantly influence the effect of selection on the quantitative character. 相似文献
992.
993.
994.
Ryoichi Sato Masahiko Kobayashi Hitoshi Watanabe 《Journal of invertebrate pathology》1982,40(2):260-265
Nosema bombycis, two Nosema spp., and a Pleistophora sp. were propagated in the silkworm and the fine structures of their spores were studied. The morphology of the polaroplast, the appearance of the nucleus, and the number of coils in the polar filament differed among the spores of the four species. The spores of the three Nosema species, however, had several identical components; e.g., the polaroplast was made up of two parts, they had two nuclei, and the ribosome arrangement was similar. On the other hand, the spore of Pleistophora sp. had a polaroplast composed of three parts, a single nucleus, and ribosomes arranged around the polar filament. Thus the fine structures of the spore differentiate microsporidan species. 相似文献
995.
K Osanai K Takahashi S Sato K Iwabuchi K Ohtake M Sata S Yasui 《Journal of applied physiology》1991,70(3):1300-1308
We investigated whether alveolar surface force increased and participated in the lung pressure-volume relationship in bleomycin-induced pulmonary fibrosis in hamsters and, if so, whether lung surfactant was hampered in the lungs. On the air-filled pressure-volume curve, decreases of lung volume from control level were significantly higher at 3-8 cmH2O pressure on day 10 than on day 30. Because the change of lung tissue elasticity evaluated from the saline-filled pressure-volume curve was equal for the 2 days, the higher decrease of air volume on day 10 was due primarily to contribution of alveolar surface force. Pressure differences between deflation limbs of air-filled and saline-filled pressure-volume curves, which represented net alveolar surface force, were significantly higher at any lung volume between 50 and 90% total lung capacity on day 10, but almost no significance was observed on day 30. Phospholipid concentration in bronchoalveolar lavage fluid significantly decreased on day 10 but had improved by day 30. Analysis of phospholipid species in purified lung surfactant showed decreased fractions of disaturated phosphatidylcholine and phosphatidylglycerol on day 10. Surface-active properties of the surfactant, measured by a modified Wilhelmy balance, were remarkably hampered on day 10, but most of them had improved by day 30. We consider that the quantitative and functional abnormalities of lung surfactant have a part in the aggravation of lung mechanics in the acute phase of pulmonary fibrosis. 相似文献
996.
Sato Takahide; Kusaba Sakiku; Nakagawa Hiroki; Ogura Nagao 《Plant & cell physiology》1984,25(6):1069-1071
By using a monospecific anti-polygalacturonase-2 antibody, a54K-dalton polypeptide was detected in in vitro translationproducts by a wheat germ cell-free translation system programmedwith polyadenylated RNA from ripe tomato pericarp tissue. Thisputative precursor of polygalacturonase was about 9K daltonslarger in molecular weight than polygalacturonase-2. (Received December 12, 1983; Accepted May 17, 1984) 相似文献
997.
Background
We have previously demonstrated that the chronic intervention in the cholinergic system by donepezil, an acetylcholinesterase inhibitor, plays a beneficial role in suppressing long-term cardiac remodeling after myocardial infarction (MI). In comparison with such a chronic effect, however, the acute effect of donepezil during an acute phase of MI remains unclear. Noticing recent findings of a cholinergic mechanism for anti-inflammatory actions, we tested the hypothesis that donepezil attenuates an acute inflammatory tissue injury following MI.Methods and Results
In isolated and activated macrophages, donepezil significantly reduced intra- and extracellular matrix metalloproteinase-9 (MMP-9). In mice with MI, despite the comparable values of heart rate and blood pressure, the donepezil-treated group showed a significantly lower incidence of cardiac rupture than the untreated group during the acute phase of MI. Immunohistochemistry revealed that MMP-9 was localized at the infarct area where a large number of inflammatory cells including macrophages infiltrated, and the expression and the enzymatic activity of MMP-9 at the left ventricular infarct area was significantly reduced in the donepezil-treated group.Conclusion
The present study suggests that donepezil inhibits the MMP-9-related acute inflammatory tissue injury in the infarcted myocardium, thereby reduces the risk of left ventricular free wall rupture during the acute phase of MI. 相似文献998.
Cortical maps, consisting of orderly arrangements of functional columns, are a hallmark of the organization of the cerebral cortex. However, the microorganization of cortical maps at the level of single neurons is not known, mainly because of the limitations of available mapping techniques. Here, we used bulk loading of Ca2+ indicators combined with two-photon microscopy to image the activity of multiple single neurons in layer (L) 2/3 of the mouse barrel cortex in vivo. We developed methods that reliably detect single action potentials in approximately half of the imaged neurons in L2/3. This allowed us to measure the spiking probability following whisker deflection and thus map the whisker selectivity for multiple neurons with known spatial relationships. At the level of neuronal populations, the whisker map varied smoothly across the surface of the cortex, within and between the barrels. However, the whisker selectivity of individual neurons recorded simultaneously differed greatly, even for nearest neighbors. Trial-to-trial correlations between pairs of neurons were high over distances spanning multiple cortical columns. Our data suggest that the response properties of individual neurons are shaped by highly specific subcolumnar circuits and the momentary intrinsic state of the neocortex. 相似文献
999.
Moreira TS Sato MA Takakura AC Menani JV Colombari E 《American journal of physiology. Regulatory, integrative and comparative physiology》2005,289(5):R1416-R1425
In the present study, we investigated the effects of inhibition of the caudal ventrolateral medulla (CVLM) with the GABA(A) agonist muscimol combined with the blockade of glutamatergic mechanism in the nucleus of the solitary tract (NTS) with kynurenic acid (kyn) on mean arterial pressure (MAP), heart rate (HR), and regional vascular resistances. In male Holtzman rats anesthetized intravenously with urethane/chloralose, bilateral injections of muscimol (120 pmol) into the CVLM or bilateral injections of kyn (2.7 nmol) into the NTS alone increased MAP to 186 +/- 11 and to 142 +/- 6 mmHg, respectively, vs. control: 105 +/- 4 mmHg; HR to 407 +/- 15 and to 412 +/- 18 beats per minute (bpm), respectively, vs. control: 352 +/- 12 bpm; and renal, mesenteric and hindquarter vascular resistances. However, in rats with the CVLM bilaterally blocked by muscimol, additional injections of kyn into the NTS reduced MAP to 88 +/- 5 mmHg and mesenteric and hindquarter vascular resistances below control baseline levels. Moreover, in rats with the glutamatergic mechanisms of the NTS blocked by bilateral injections of kyn, additional injections of muscimol into the CVLM also reduced MAP to 92 +/- 2 mmHg and mesenteric and hindquarter vascular resistances below control baseline levels. Simultaneous blockade of NTS and CVLM did not modify the increase in HR but also abolished the increase in renal vascular resistance produced by each treatment alone. The results suggest that important pressor mechanisms arise from the NTS and CVLM to control vascular resistance and arterial pressure under the conditions of the present study. 相似文献
1000.
A host-mediated RNA-editing event allows hepatitis delta virus (HDV) to express two essential proteins, the small delta antigen (HDAg-S) and the large delta antigen (HDAg-L), from a single open reading frame. One or several members of the ADAR (adenosine deaminases that act on RNA) family are thought to convert the adenosine to an inosine (I) within the HDAg-S amber codon in antigenomic RNA. As a consequence of replication, the UIG codon is converted to a UGG (tryptophan [W]) codon in the resulting HDAg-L message. Here, we used a novel reporter system to monitor the editing of the HDV amber/W site in the absence of replication. In cultured cells, we observed that both human ADAR1 (hADAR1) and hADAR2 were capable of editing the amber/W site with comparable efficiencies. We also defined the minimal HDV substrate required for hADAR1- and hADAR2-mediated editing. Only 24 nucleotides from the amber/W site were sufficient to enable efficient editing by hADAR1. Hence, the HDV amber/W site represents the smallest ADAR substrate yet identified. In contrast, the minimal substrate competent for hADAR2-mediated editing contained 66 nucleotides. 相似文献