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371.
372.
Shin Masateru; Sakihama Naoko; Koike Hiroyuki; Inoue Yorinao 《Plant & cell physiology》1984,25(8):1575-1578
Two C-type cytochromes, c-550 and c-553, were extracted by animproved procedure from a thermophilic blue-green alga, Synechococcusvulcanus, and effectively purified by a two-step hydrophobicchromatography. The first step was performed with a ToyopearlHW-65C :ammonium sulfate-66 column and the second with a butyl-Toyopearl650 column. This work is the first to apply butyl-toyopearl650 to protein purification. (Received July 2, 1984; Accepted September 13, 1984) 相似文献
373.
374.
Shunji Takahashi Wai-Cheung Yip Toshiko Matsugami Goro Tamura 《Journal of plant research》1996,109(4):363-368
The ferredoxin-sulfite reductase (Fd-SiR; hydrogen-sulfide: ferredoxin oxidoreductase, EC 1.8.7.1) activities of shoot and
root of leek (Allium tuberosum) were increased by sulfate limitation in the early stage of growth. Western blot analysis demonstrated an increased amount
of SiRs in root under sulfate limitation, suggesting that SiRs were derepressed. The derepression was observed in shoot when
1.5 mM nitrate was supplied to the plants under sulfate limitation, and clearly in root when 15 mM nitrate was supplied under
sulfate limitation. When nitrate was absent from the nutrient solution, the SiR activity in both tissues was very low. Combined
with the results of the sulfate- or nitrate-limitation experiments, it is suggested that the degree of the derepression of
SiR in both tissue under sulfate limitation is affected by the concentration of nitrate, and further that the mechanism of
regulation of the SiR activity is different in each tissue. The decreases in the ratios of the total SiR activities (shoot/root)
in the latter stage of seedling growth indicate that root play a very important role in sulfate assimilation. 相似文献