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81.
Hiroaki Nobuhara Keisuke Kuida Makoto Furutani Toshihiko Shiroishi Kazuo Moriwaki Yusuke Yanagi Tomio Tada 《Immunogenetics》1989,30(6):405-413
Southern blots of genomic DNA from 23 strains of laboratory mice and 19 individual wild mice were examined for restriction
fragment length polymorphisms in their loci encoding the T-cell receptors (Tcr): the constant regions of the α, β, and γ chains
(C
α,C
β, andC
γ) and a variable region family of the β chain (V
β8). Only a few polymorphisms were observed for each locus in the laboratory mice after using three restriction enzymes,Bam HI,Eco RI, andHind III. All the laboratory mice examined fall into one of two types for theC
α,C
β andV
β8 loci and one of three types for theC
γ. These types are found in some of the wild mice studied, indicating that they were already present in the founder mice of
laboratory mouse strains. In contrast, theTcr genes are highly polymorphic among wild mice. Analysis of the polymorphisms in these loci suggests that laboratory mice have
inherited their genes not only fromMus musculus domesticus, but also from other subspecies, and much more than previously believed from Asian subspecies. 相似文献
82.
83.
Koichiro Yamada Tomonori Tsukahara Kazuhisa Yoshino Katsuhiko Kojima Hideyuki Agawa Yuki Yamashita Yuji Amano Mariko Hatta Yasunori Matsuzaki Naoki Kurotori Keiko Wakui Yoshimitsu Fukushima Ryosuke Osada Tanri Shiozawa Kazuo Sakashita Kenichi Koike Satoru Kumaki Nobuyuki Tanaka Toshikazu Takeshita 《Retrovirology》2009,6(1):1-9
84.
Ohnogi H Kudo Y Tahara K Sugiyama K Enoki T Hayami S Sagawa H Tanimura Y Aoi W Naito Y Kato I Yoshikawa T 《Bioscience, biotechnology, and biochemistry》2012,76(5):961-966
Angelica keiskei (Ashitaba in Japanese), a traditional herb in Japan, contains abundant prenylated chalcones. It has been reported that the chalcones from A. keiskei showed such bioactivities as anti-bacterial, anti-cancer and anti-diabetic effects. Xanthoangelol, 4-hydroxyderricin and six new chalcones were isolated in this study from an ethanol extract of A. keiskei by octadecyl silyl (ODS) and silica gel chromatography, and identified by 1D- and 2D-nuclear magnetic resonance (NMR) and high-resolution mass spectrometric analyses. The chalcones from A. keiskei markedly increased the expression of the adiponectin gene and the production of adiponectin in 3T3-L1 adipocytes. These results suggest that the chalcones from A. keiskei might be useful for preventing the metabolic syndrome. 相似文献
85.
RUNX3 suppresses gastric epithelial cell growth by inducing p21(WAF1/Cip1) expression in cooperation with transforming growth factor {beta}-activated SMAD 总被引:3,自引:0,他引:3 下载免费PDF全文
Chi XZ Yang JO Lee KY Ito K Sakakura C Li QL Kim HR Cha EJ Lee YH Kaneda A Ushijima T Kim WJ Ito Y Bae SC 《Molecular and cellular biology》2005,25(18):8097-8107
86.
87.
Kishimoto E Naito Y Handa O Okada H Mizushima K Hirai Y Nakabe N Uchiyama K Ishikawa T Takagi T Yagi N Kokura S Yoshida N Yoshikawa T 《American journal of physiology. Gastrointestinal and liver physiology》2011,301(2):G230-G238
Human esophageal epithelium is continuously exposed to physical stimuli or to gastric acid that sometimes causes inflammation of the mucosa. Transient receptor potential vanilloid 1 (TRPV1) is a nociceptive, Ca(2+)-selective ion channel activated by capsaicin, heat, and protons. It has been reported that activation of TRPV1 expressed in esophageal mucosa is involved in gastroesophageal reflux disease (GERD) or in nonerosive GERD symptoms. In this study, we examined the expression and function of TRPV1 in the human esophageal epithelial cell line Het1A, focusing in particular on the role of oxidative stress. Interleukin-8 (IL-8) secreted by Het1A cells upon stimulation by capsaicin or acid with/without 4-hydroxy-2-nonenal (HNE) was measured by ELISA. Following capsaicin stimulation, the intracellular production of reactive oxygen species (ROS) was determined using a redox-sensitive fluorogenic probe, and ROS- and HNE-modified proteins were determined by Western blotting using biotinylated cysteine and anti-HNE antibody, respectively. HNE modification of TRPV1 proteins was further investigated by immunoprecipitation after treatment with synthetic HNE. Capsaicin and acid induced IL-8 production in Het1A cells, and this production was diminished by antagonists of TRPV1. Capsaicin also significantly increased the production of intracellular ROS and ROS- or HNE-modified proteins in Het1A cells. Moreover, IL-8 production in capsaicin-stimulated Het1A cells was enhanced by synthetic HNE treatment. Immunoprecipitation studies revealed that TRPV1 was modified by HNE in synthetic HNE-stimulated Het1A cells. We concluded that TRPV1 functions in chemokine production in esophageal epithelial cells, and this function may be regulated by ROS via posttranslational modification of TRPV1. 相似文献
88.
Mariko Hiraiwa-Hasegawa Toshikazu Hasegawa Toshisada Nishida 《Primates; journal of primatology》1984,25(4):401-413
Long-term demographic observations on a large-sized unit-group of chimpanzees in the Mahale Mountains, Tanzania, are summarized.
The unit-group, the M group, contains over 100 individuals, which makes it the largest unit-group ever reported. The age-sex
composition, natality, mortality and transfers of the M group are analyzed. An attempt is made to illustrate an age-sex pyramid
of the group by estimating the ages of all the individuals in the group. The results reveal that: (1) the mortality rate of
the male infants within 1 year almost doubled that of female infant; (2) adult male to adult female ratio of the M group is
considerably higher than any other unit-groups elsewhere; and (3) the M group contains a relatively large number of old animals
over 40 years of age, suggesting that the longevity of wild chimpanzees might be greater than estimated so far. 相似文献
89.
Bronchioalveolar morphogenesis of human bronchial epithelial cells depending upon hepatocyte growth factor 下载免费PDF全文
Takashi Kato Kiyomasa Oka Toshikazu Nakamura Akihiko Ito 《Journal of cellular and molecular medicine》2015,19(12):2818-2826
Lung alveolar regeneration occurs in adult human lungs as a result of proliferation, differentiation and alveolar morphogenesis of stem cells. It is increasingly being believed that bronchial epithelial cells (BECs) have a potential as stem cells, because they are potent to differentiate into multiple central and peripheral lung cell types in three‐dimensional (3D) cultures, and they develop multiple foci with well‐differentiated histogenesis after transformed into neoplastic cells. In this study, we investigated morphogenic abilities of HBE135 human BECs immortalized by E6/E7 oncogene in 3D cultures. When HBE135 cells were cultured alone or co‐cultured with endothelial cells, the cells formed spherical colonies without branching. However, in co‐culture with lung fibroblast MRC‐9 cells, HBE135 cells formed colonies with bronchioalveolar‐like complex branching, suggesting that MRC‐9‐derived soluble factor(s) are responsible for the branching formation. MRC‐9 cells, not endothelial cells, were found to highly express hepatocyte growth factor (HGF), a soluble molecule involved in liver and kidney regeneration. An anti‐HGF neutralizing antibody severely suppressed the complex branching formation, but addition of HGF could not sufficiently compensate the morphogenic effects of MRC‐9 cells, suggesting that MCR‐9‐derived HGF was necessary but insufficient for the bronchioalveolar structure formation. Immunohistochemistry revealed that Met, a cognate receptor for HGF, was highly expressed and phosphorylated in neoplastic BECs from lung adenocarcinomas with well‐differentiated, not poorly differentiated, histogenesis. These results are consistent with the notion that BECs have an aspect of stem cells. This aspect appears to become manifest through HGF–Met signalling pathway activation. 相似文献
90.
Aoi W Naito Y Nakamura T Akagiri S Masuyama A Takano T Mizushima K Yoshikawa T 《The Journal of nutritional biochemistry》2007,18(2):140-145
Milk fermented with a starter containing Lactobacillus helveticus and Saccharomyces cerevisiae is drunk on a daily basis by many people in Japan and has several beneficial effects. We studied the influence of this fermented milk product on muscle damage after prolonged exercise in rats. Wistar rats were divided into four groups: rested controls, rested rats given fermented milk diet, exercised rats and exercised rats given fermented milk diet. After 3 weeks of acclimatization, both exercise groups were made to run on a treadmill at 26 m/min for 60 min. Exercise increased the serum creatine kinase level, as well as myeloperoxidase activity and the level of thiobarbituric-acid-reactive substances in the gastrocnemius muscle after 24 h. These changes were ameliorated by intake of fermented milk. An increase of CINC-1 was also ameliorated by fermented milk. Furthermore, milk diet increased the mRNA and protein levels of protective proteins such as antioxidants and chaperone proteins. These results indicate that fermented milk can ameliorate delayed-onset muscle damage after prolonged exercise, which is associated with an increased antioxidant capacity of muscles. 相似文献