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131.
Genome‐wide transcriptome analysis of Aspergillus fumigatus exposed to osmotic stress reveals regulators of osmotic and cell wall stresses that are SakAHOG1 and MpkC dependent 下载免费PDF全文
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Saito F Saito-Arai Y Nakamura-Okuma A Ikeda M Hagiwara H Masaki T Shimizu T Matsumura K 《Biochemical and biophysical research communications》2011,(2):494-369
α-Dystroglycan (α-DG) plays crucial roles in maintaining the stability of cells. We demonstrated previously that the N-terminal domain of α-DG (α-DG-N) is secreted by cultured cells into the culture medium. In the present study, to clarify its function in vivo, we generated a monoclonal antibody against α-DG-N and investigated the secretion of α-DG-N in human cerebrospinal fluid (CSF). Interestingly, we found that a considerable amount of α-DG-N was present in CSF. α-DG-N in CSF was a sialylated glycoprotein with both N- and O-linked glycan. These observations suggest that secreted α-DG-N may be transported via CSF and have yet unidentified effects on the nervous system. 相似文献
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Koushirou?SugaEmail author Yukari?Tanaka Yoshitaka?Sakakura Atsushi?Hagiwara 《Hydrobiologia》2011,662(1):113-119
The rotifer Brachionus plicatilis culture is composed of complex microcosms including bacteria, protozoans, algae, and fungi. Previous studies reported methods
to establish axenic rotifer cultures, but further refinement of these techniques is needed, for molecular biological research
which requires pure culture to isolate nucleic acids from rotifers only. In order to render rotifer culture axenic, we tested
five antibiotics: ampicillin (Amp), chloramphenicol (Cp), kanamycin (Km), nalidixic acid (Na), and streptomycin (Sm) at 30–100 μg/ml.
Except for Cp, which reduces rotifer reproduction, all other antibiotics at the tested concentrations did not affect rotifer
reproduction or show any toxic effects. A rotifer disinfection method was finally established by treating the resting eggs
with 0.25% (w/v) sodium hypochlorite (NaOCl) for 3 min, washing with sterilized sea water, and then exposing the neonates
to an Amp, Km, Na, and Sm mixture. Using four nutrient media, we confirmed that this protocol renders the rotifer culture
bacterial and fungus free. The axenic rotifer culture generated here is useful not only for genetic analysis of Brachionus plicatilis, but for studying the rotifer life cycle without bacterial influence. 相似文献
135.
Jae-Seong Lee Ryeo-Ok Kim Jae-Sung Rhee Jeonghoon Han Dae-Sik Hwang Beom-Soon Choi Chang Joo Lee Yong-Dal Yoon Jong-Sung Lim Young-Mi Lee Gyung Soo Park Atsushi Hagiwara Ik-Young Choi 《Hydrobiologia》2011,662(1):65-75
The monogonont rotifer, Brachionus ibericus (S type), is considered to be a promising model species for developmental biology, evolution, and environmental genomics. In an attempt to accelerate the molecular understanding of B. ibericus, we sequenced 680.5 Mb of genomic DNA using the genome sequencer GS-FLX-Titanium. We obtained 2,062,621 reads (average read length 329.9 bp) and 145,418 contigs (total contigs length 125.7 Mb) after excluding small reads (less than 200 bp) from the assembly, and finally obtained 10,133 unigenes (E value ?? 9.00E?04) after non-redundant (NR) BLAST search. In this article, we summarize the genomic DNA sequences of B. ibericus and discuss their potential use in the study of reproductive biology, endocrinology, environmental genomics, and ecotoxicological studies, and for providing insight into the genetic basis of mechanisms such as egg formation, antioxidant stress defense, and xenobiotic metabolism. 相似文献
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Masahiro Tsuchiya Fukie Niijima-Yaoita Hiroyuki Yoneda Ko Chiba Shinobu Tsuchiya Yoshihiro Hagiwara Keiichi Sasaki Shunji Sugawara Yasuo Endo Koichi Tan-No Makoto Watanabe 《Life sciences》2014