全文获取类型
收费全文 | 539篇 |
免费 | 44篇 |
出版年
2020年 | 2篇 |
2019年 | 2篇 |
2018年 | 4篇 |
2017年 | 3篇 |
2016年 | 11篇 |
2015年 | 14篇 |
2014年 | 14篇 |
2013年 | 29篇 |
2012年 | 27篇 |
2011年 | 37篇 |
2010年 | 16篇 |
2009年 | 14篇 |
2008年 | 34篇 |
2007年 | 46篇 |
2006年 | 26篇 |
2005年 | 33篇 |
2004年 | 27篇 |
2003年 | 24篇 |
2002年 | 23篇 |
2001年 | 14篇 |
2000年 | 12篇 |
1999年 | 10篇 |
1998年 | 5篇 |
1997年 | 7篇 |
1996年 | 5篇 |
1995年 | 3篇 |
1993年 | 9篇 |
1992年 | 9篇 |
1991年 | 7篇 |
1990年 | 7篇 |
1989年 | 6篇 |
1988年 | 4篇 |
1987年 | 11篇 |
1986年 | 6篇 |
1985年 | 3篇 |
1984年 | 9篇 |
1983年 | 10篇 |
1982年 | 4篇 |
1981年 | 9篇 |
1980年 | 3篇 |
1979年 | 7篇 |
1978年 | 5篇 |
1977年 | 2篇 |
1976年 | 4篇 |
1975年 | 6篇 |
1974年 | 2篇 |
1973年 | 4篇 |
1972年 | 4篇 |
1971年 | 2篇 |
1968年 | 3篇 |
排序方式: 共有583条查询结果,搜索用时 15 毫秒
91.
Atsushi Okazawa Chitra Trakulnaleamsai Hiroya Hiramatsu Ei'ichiro Fukusaki Koichi Yoneyama Yasutomo Takeuchi Akio Kobayashi 《Plant Physiology and Biochemistry》2005,43(5):499-502
Orobanche minor is a non-photosynthetic root holoparasitic plant. Although it is known that photosynthesis-related genes are inactivated or have been eliminated from the plastid genomes of holoparasites, little is known about the alterations in their genes involved in the signaling networks by which light regulates photosynthesis. Cryptochromes (crys), which are blue-light receptors, appear to control both photosynthesis-related and non-photosynthetic responses to light in higher plants. Because we are interested in to what extent a cry-mediated light signaling network remains in the holoparasites, we cloned CRY homologous cDNA from O. minor (OmCRY1) and used real-time RT-PCR to compare its expression under natural daylight and darkness. We found that the OmCRY1 has a high degree of homology with CRY1 s from photosynthetic plants. Expression of the OmCRY1 gene was higher in plants grown in the dark than that in the plants grown under natural daylight. This is the first report of the gene expression of a blue-light receptor in non-photosynthetic plants. 相似文献
92.
93.
94.
95.
Terasaki N Yamamoto N Tamada K Hattori M Hiraga T Tohri A Sato I Iwai M Iwai M Taguchi S Enami I Inoue Y Yamanoi Y Yonezawa T Mizuno K Murata M Nishihara H Yoneyama S Minakata M Ohmori T Sakai M Fujii M 《Biochimica et biophysica acta》2007,1767(6):653-659
We report on the first successful output of electrons directly from photosystem I (PSI) of thermophilic cyanobacteria to the gate of a field-effect transistor (FET) by bypassing electron flow via a newly designed molecular wire, i.e., artificial vitamin K(1), and a gold nanoparticle; in short, this newly manufactured photosensor employs a bio-functional unit as the core of the device. Photo-electrons generated by the irradiation of molecular complexes composed of reconstituted PSI on the gate were found to control the FET. This PSI-bio-photosensor can be used to interpret gradation in images. This PSI-FET system is moreover sufficiently stable for use exceeding a period of 1 year. 相似文献
96.
97.
Nao Terasaki Kaoru Tamada Takashi Hiraga Akihiko Tohri Masako Iwai Shunpei Taguchi Yasunori Inoue Yoshinori Yamanoi Katsuya Mizuno Hiroshi Nishihara Satoshi Yoneyama Tsutomu Ohmori Masaaki Fujii 《BBA》2007,1767(6):653-659
We report on the first successful output of electrons directly from photosystem I (PSI) of thermophilic cyanobacteria to the gate of a field-effect transistor (FET) by bypassing electron flow via a newly designed molecular wire, i.e., artificial vitamin K1, and a gold nanoparticle; in short, this newly manufactured photosensor employs a bio-functional unit as the core of the device. Photo-electrons generated by the irradiation of molecular complexes composed of reconstituted PSI on the gate were found to control the FET. This PSI-bio-photosensor can be used to interpret gradation in images. This PSI-FET system is moreover sufficiently stable for use exceeding a period of 1 year. 相似文献
98.
FGF-2 suppresses cellular senescence of human mesenchymal stem cells by down-regulation of TGF-beta2 总被引:2,自引:0,他引:2
Ito T Sawada R Fujiwara Y Seyama Y Tsuchiya T 《Biochemical and biophysical research communications》2007,359(1):108-114
Human mesenchymal stem cells (hMSCs) are able to both self-replicate and differentiate into a variety of cell types. Fibroblast growth factor-2 (FGF-2) stimulates the growth of hMSCs in vitro, but its mechanisms have not been clarified yet. In this study, we investigated whether cellular senescence was involved in the stimulation of hMSCs growth by FGF-2 and the expression levels of transforming growth factor-beta1 and -beta2 (TGF-betas). Because hMSCs were induced cellular senescence due to long-term culture, FGF-2 decreased the percentage of senescent cells and suppressed G1 cell growth arrest through the suppression of p21(Cip1), p53, and p16(INK4a) mRNA expression levels. Furthermore, the levels of TGF-betas mRNA expression in hMSCs were increased by long-term culture, but FGF-2 suppressed the increase of TGF-beta2 mRNA expression due to long-term culture. These results suggest that FGF-2 suppresses the hMSCs cellular senescence dependent on the length of culture through down-regulation of TGF-beta2 expression. 相似文献
99.
A Saito T Fujii T Yoneyama M Redenbach T Ohno T Watanabe K Miyashita 《Bioscience, biotechnology, and biochemistry》1999,63(4):710-718
Six different genes for chitinase from ordered cosmids of the chromosome of Streptomyces coelicolor A3(2) were identified by hybridization, using the chitinase genes from other Streptomyces spp. as probes, and cloned. The genes were sequenced and analyzed. The genes, together with an additional chitinase gene obtained from the data bank, can be classified into either family 18 or family 19 of the glycosyl hydrolase classification. The five chitinases that fall into family 18 show diversity in their multiple domain structures as well as in the amino acid sequences of their catalytic domains. The remaining two chitinases are members of family 19 chitinases, since their C-terminus shares more than 70% identity with the catalytic domain of ChiC of Streptomyces griseus, the sole gene for family 19 chitinase so far found in an organism other than higher plants. 相似文献
100.
Kiyohiro Takahasi Hiroyuki Kumeta Natsuko Tsuduki Ryo Narita Taeko Shigemoto Reiko Hirai Mitsutoshi Yoneyama Masataka Horiuchi Kenji Ogura Takashi Fujita Fuyuhiko Inagaki 《The Journal of biological chemistry》2009,284(26):17465-17474
The RIG-I like receptor (RLR) comprises three homologues: RIG-I (retinoic acid-inducible gene I), MDA5 (melanoma differentiation-associated gene 5), and LGP2 (laboratory of genetics and physiology 2). Each RLR senses different viral infections by recognizing replicating viral RNA in the cytoplasm. The RLR contains a conserved C-terminal domain (CTD), which is responsible for the binding specificity to the viral RNAs, including double-stranded RNA (dsRNA) and 5′-triphosphated single-stranded RNA (5′ppp-ssRNA). Here, the solution structures of the MDA5 and LGP2 CTD domains were solved by NMR and compared with those of RIG-I CTD. The CTD domains each have a similar fold and a similar basic surface but there is the distinct structural feature of a RNA binding loop; The LGP2 and RIG-I CTD domains have a large basic surface, one bank of which is formed by the RNA binding loop. MDA5 also has a large basic surface that is extensively flat due to open conformation of the RNA binding loop. The NMR chemical shift perturbation study showed that dsRNA and 5′ppp-ssRNA are bound to the basic surface of LGP2 CTD, whereas dsRNA is bound to the basic surface of MDA5 CTD but much more weakly, indicating that the conformation of the RNA binding loop is responsible for the sensitivity to dsRNA and 5′ppp-ssRNA. Mutation study of the basic surface and the RNA binding loop supports the conclusion from the structure studies. Thus, the CTD is responsible for the binding affinity to the viral RNAs. 相似文献