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排序方式: 共有137条查询结果,搜索用时 31 毫秒
21.
22.
Liang Zhang Xu Liang Zhihe Zhang Fujun Shen Wenping Zhang Kun Wei Zhi Yang Rong Hou Bisong Yue Hiroshi Kamata Ken Okabayashi Shigeo Ohba Hideo Kiba Shigehisa Tsumagari Tsuneo Sato Kiichi Kanayama Injen Pan Toshi Watanabe 《Conservation Genetics》2008,9(3):787-790
Ten polymorphic microsatellite loci were characterized from two genomic DNA-enriched libraries of the red panda (Ailurus fulgens). The number of observed alleles among 35 samples of red pandas ranged from five to 12. Observed and expected heterozygosities
were 0.286–0.971 and 0.443–0.894, and the mean polymorphic information content was 0.712. All loci followed Hardy–Weinberg
expectations except Aifu-14 and Aifu-16, which may due to the presence of inbreeding or null alleles. Three pairs of loci
exhibited significant linkage disequilibrium after Bonferroni correction for multiple comparisons. These microsatellites would
be useful to strengthen population management, genetic diversity exploration, and demographic history speculation of this
species. 相似文献
23.
Nagata T Zharmukhamedov SK Khorobrykh AA Klimov VV Allakhverdiev SI 《Photosynthesis research》2008,98(1-3):277-284
Reconstitution of Mn-depleted photosystem II (PSII) particles was examined with synthetic trinuclear Mn complexes of newly developed tripod ligands. Rates of the electron transfer and oxygen evolution were up to 74-86 and 52-56% of those measured in native PSII. These values are higher than those for the PSII reconstituted by MnCl(2). The role of the tripod ligands during the reconstitution process was examined by (19)F NMR. Due to the high NMR sensitivity of the (19)F nucleus and the low abundance of fluorine atoms in natural PSII, it was possible to selectively observe the fluorine atoms on the tripod ligand. It was shown that the tripod ligands were released from the Mn complex after the reconstitution. We propose that the primary step in the reconstitution process is the prebinding of the Mn complex to the hydrophobic part of the PSII particle. 相似文献
24.
Chiaki Motegi Toshi Nagata Takeshi Miki Markus G. Weinbauer Louis Legendre Fereidoun Rassoulzadegan 《PloS one》2013,8(11)
A general model of species diversity predicts that the latter is maximized when productivity and disturbance are balanced. Based on this model, we hypothesized that the response of bacterial diversity to the ratio of viral to bacterial production (VP/BP) would be dome-shaped. In order to test this hypothesis, we obtained data on changes in bacterial communities (determined by terminal restriction fragment length polymorphism of 16S rRNA gene) along a wide VP/BP gradient (more than two orders of magnitude), using seawater incubations from NW Mediterranean surface waters, i.e., control and treatments with additions of phosphate, viruses, or both. In December, one dominant Operational Taxonomic Unit accounted for the major fraction of total amplified DNA in the phosphate addition treatment (75±20%, ± S.D.), but its contribution was low in the phosphate and virus addition treatment (23±19%), indicating that viruses prevented the prevalence of taxa that were competitively superior in phosphate-replete conditions. In contrast, in February, the single taxon predominance in the community was held in the phosphate addition treatment even with addition of viruses. We observed statistically robust dome-shaped response patterns of bacterial diversity to VP/BP, with significantly high bacterial diversity at intermediate VP/BP. This was consistent with our model-based hypothesis, indicating that bacterial production and viral-induced mortality interactively affect bacterial diversity in seawater. 相似文献
25.
Electrical Activity and Histological Change in the Degenerating Olfactory Epithelium 总被引:4,自引:3,他引:1
Electrical activity and histological changes were studied in the degenerating olfactory epithelium of the bullfrog after the olfactory nerve had been sectioned. After nerve section, the electrical responses to odors disappeared in the olfactory epithelium in 8 days in the summer, in 11 days in the early autumn, and in 16 days in the early winter. In the degenerating olfactory epithelium a striking decrease in the number of olfactory cells was found, but not of supporting cells. The ratio of the number of olfactory cells to that of supporting cells was found to decrease from 5 or 6 to below 2 after the nerve section. At a ratio below 2, the electrical responses to odor disappeared. The histological changes in the bullfrog are compared with those in the mouse and rabbit. The localization of the olfactory pigment and the electrical activity of the supporting cell are discussed. It was concluded that all three types of responses to odors originate from the activity of the olfactory cell. 相似文献
26.
Separation of novel phosphoproteins of Porphyromonas gingivalis using phosphate‐affinity chromatography
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Masashi Izumigawa Yoshiaki Hasegawa Ryota Ikai Toshi Horie Megumi Inomata Takeshi Into Noriyuki Kitai Fuminobu Yoshimura Yukitaka Murakami 《Microbiology and immunology》2016,60(10):702-707
Phosphorylation of serine, threonine and tyrosine is a central mechanism for regulating the structure and function of proteins in both eukaryotes and prokaryotes. However, the action of phosphorylated proteins present in Porphyromonas gingivalis, a major periodontopathogen, is not fully understood. Here, six novel phosphoproteins that possess metabolic activities were identified, namely PGN_0004, PGN_0375, PGN_0500, PGN_0724, PGN_0733 and PGN_0880, having been separated by phosphate‐affinity chromatography. The identified proteins were detectable by immunoblotting specific to phosphorylated Ser (P‐Ser), P‐Thr, and/or P‐Tyr. These results imply that novel phosphorylated proteins might play an important role for regulation of metabolism in P. gingivalis. 相似文献
27.
The synthesis and electrochemistry of half-sandwich type of Co(III) complexes [(C5Me5)Co(bidentate)(CH3CN)](BF4)2 {bidentate = dppe (1,2-bis(diphenylphosphino)ethane), dppp (1,3-bis(diphenylphosphino)propane), bpy (2,2′-bipyridine), en (ethylenediamine)) are reported. Cyclic voltammograms of [(C5Me5)Co(bidentate)(CH3CN)](BF4)2 in CH3CN s showed two redox couples assignable to Co(II)/Co(III) and Co(I)/Co(II). The Co(I) complex having C5Me5 and dppe was also prepared. Two redox couples of this Co(I) complex, (C5Me5)Co(dppe), in CH3CN coincided with those of [(C5Me5)Co(dppe)(CH3CN)](BF4)2 in spite of the structural change around the metal center. 相似文献
28.
An E. coli expression system optimized for DELLA proteins 总被引:1,自引:0,他引:1
Sun X Frearson N Kirk C Jones WT Harvey D Rakonjac J Foster T Al-Samarrai T 《Protein expression and purification》2008,58(1):168-174
The DELLA proteins are involved in regulation of plant growth in response to phytohormonal signals such as GA, ethylene, and auxin. They have become one of most challenging and active area of research due to their fundamental roles in plant biology. Here, we describe the first successful expression of the N-terminal domains of DELLA proteins of Arabidopsis thaliana and Malus domestica in Escherichia coli system which will be used to produce monoclonal antibodies for profiling protein micro-arrays. Optimizations of the cloning, expression, and purification using specific tags have been discussed. 相似文献
29.
Nakatani Y Hotta S Utsunomiya I Tanaka K Hoshi K Ariga T Yu RK Miyatake T Taguchi K 《Neurochemical research》2009,34(1):149-157
To investigate the pathophysiological mechanisms of immune-mediated peripheral neuropathies, we studied the effects of sera
from patients with Guillain-Barré syndrome (GBS) on the Cav2.1 voltage-dependent calcium channel (VDCC) current in Purkinje
cells. Using the whole-cell recording technique, Cav2.1 VDCC current was measured in cerebellar Purkinje cells in the presence
of serum from GBS patients with acute motor axonal neuropathy (AMAN) or acute inflammatory demyelinating polyneuropathy (AIDP).
The AMAN patient sera significantly inhibited the Cav2.1 VDCC current compared with healthy volunteer sera, and this inhibition
was fully reversible by washing out the AMAN serum. Similarly, IgG purified from AMAN sera also inhibited the Cav2.1 VDCC
current. However, the activation and inactivation kinetics of the Cav2.1 VDCC currents were not affected by serum from an
AMAN patient. Moreover, the VDCC current of Purkinje cells was also inhibited by IgG anti-GM1 monoclonal antibody (anti-GM1
mAb). In an immunocytochemical study using double fluorescence staining, Purkinje cells were stained by monoclonal IgG anti-GM1
mAb. In contrast, AIDP patient and healthy volunteer sera did not affect the Cav2.1 VDCC current. These results suggest that
in some case of GBS, particularly of AMAN patients with IgG anti-GM1 mAb, muscle weakness may be induced by dysfunction of
Cav2.1 VDCC functioning at the motor nerve terminals.
Special issue article in honor of Dr. George DeVries. 相似文献
30.
Nicola Aceto Aditya Bardia David T. Miyamoto Maria C. Donaldson Ben S. Wittner Joel A. Spencer Min Yu Adam Pely Amanda Engstrom Huili Zhu Brian W. Brannigan Ravi Kapur Shannon L. Stott Toshi Shioda Sridhar Ramaswamy David T. Ting Charles P. Lin Mehmet Toner Daniel A. Haber Shyamala Maheswaran 《Cell》2014