全文获取类型
收费全文 | 2762篇 |
免费 | 161篇 |
国内免费 | 1篇 |
专业分类
2924篇 |
出版年
2022年 | 15篇 |
2021年 | 38篇 |
2020年 | 16篇 |
2019年 | 17篇 |
2018年 | 31篇 |
2017年 | 29篇 |
2016年 | 50篇 |
2015年 | 115篇 |
2014年 | 128篇 |
2013年 | 192篇 |
2012年 | 168篇 |
2011年 | 180篇 |
2010年 | 115篇 |
2009年 | 133篇 |
2008年 | 173篇 |
2007年 | 166篇 |
2006年 | 195篇 |
2005年 | 192篇 |
2004年 | 217篇 |
2003年 | 178篇 |
2002年 | 154篇 |
2001年 | 27篇 |
2000年 | 22篇 |
1999年 | 40篇 |
1998年 | 37篇 |
1997年 | 31篇 |
1996年 | 21篇 |
1995年 | 23篇 |
1994年 | 21篇 |
1993年 | 20篇 |
1992年 | 14篇 |
1991年 | 13篇 |
1990年 | 14篇 |
1989年 | 8篇 |
1988年 | 17篇 |
1987年 | 7篇 |
1986年 | 7篇 |
1985年 | 11篇 |
1984年 | 9篇 |
1983年 | 8篇 |
1982年 | 12篇 |
1981年 | 15篇 |
1979年 | 8篇 |
1978年 | 8篇 |
1977年 | 3篇 |
1976年 | 8篇 |
1975年 | 2篇 |
1973年 | 2篇 |
1971年 | 3篇 |
1968年 | 2篇 |
排序方式: 共有2924条查询结果,搜索用时 15 毫秒
51.
52.
Akie Kobayashi Hye‐Jeong Kim Yuta Tomita Yutaka Miyazawa Nobuharu Fujii Sachiko Yano Chiaki Yamazaki Motoshi Kamada Haruo Kasahara Sachiko Miyabayashi Toru Shimazu Yasuo Fusejima Hideyuki Takahashi 《Physiologia plantarum》2019,165(3):464-475
Plants exhibit helical growth movements known as circumnutation in growing organs. Some studies indicate that circumnutation involves the gravitropic response, but this notion is a matter of debate. Here, using the agravitropic rice mutant lazy1 and space‐grown rice seedlings, we found that circumnutation was reduced or lost during agravitropic growth in coleoptiles. Coleoptiles of wild‐type rice exhibited circumnutation in the dark, with vigorous oscillatory movements during their growth. The gravitropic responses in lazy1 coleoptiles differed depending on the growth stage, with gravitropic responses detected during early growth and agravitropism during later growth. The nutation‐like movements observed in lazy1 coleoptiles at the early stage of growth were no longer detected with the disappearance of the gravitropic response. To verify the relationship between circumnutation and gravitropic responses in rice coleoptiles, we conducted spaceflight experiments in plants under microgravity conditions on the International Space Station. Wild‐type rice seeds were germinated, and the resulting seedlings were grown under microgravity or a centrifuge‐generated 1 g environment in space. We began filming the seedlings 2 days after seed imbibition and obtained images of seedling growth every 15 min. The seed germination rate in space was 92–100% under both microgravity and 1 g conditions. LED‐synchronized flashlight photography induced an attenuation of coleoptile growth and circumnutational movement due to cumulative light exposure. Nevertheless, wild‐type rice coleoptiles still showed circumnutational oscillations under 1 g but not microgravity conditions. These results support the idea that the gravitropic response is involved in plant circumnutation. 相似文献
53.
Andrew E Rosselot Miri Park Mari Kim Toru MatsuUra Gang Wu Danilo E Flores Krithika R Subramanian Suengwon Lee Nambirajan Sundaram Taylor R Broda Heather A McCauley Jennifer A Hawkins Kashish Chetal Nathan Salomonis Noah F Shroyer Michael A Helmrath James M Wells John B Hogenesch Sean R Moore Christian I Hong 《The EMBO journal》2022,41(2)
54.
Abliz Arkin Toru R Saito Kimimasa Takahashi Hiromi Amao Shinobu Aoki-Komori Kazuaki W Takahashi 《Experimental Animals》2003,52(1):17-24
Marking behavior, marking-like behavior [3], and changes of the scent glands were observed in aged Mongolian gerbils. In Experiment 1, changes in the marking and marking-like behavior with aging were evaluated in adult male and female Mongolian gerbils of an inbred strain aged 6 to 36 months. The frequency of marking behavior in males was significantly higher than females throughout the observation period except at 36 months of age. On the other hand, frequency of marking-like behavior in males, but not in females decreased with aging, significantly. In Experiment 2, changes of the scent gland in adult males and females aged 6 to 36 months were morphologically evaluated. Macroscopic examination revealed an increase in the size length and width of the glands of males aged 12 months and females aged 6 months. Histologically the glands of all the males and females aged 6 months developed moderately or well. Some of the 12-month-old males and females showed acinar atrophy of the glands, and all the females aged 18 months or more had highly atrophied scent glands. From these results, we concluded that there is no relationship between the changes of marking behavior and those of the scent glands in aged male Mongolian gerbils, and assume that marking behavior in aged animals does not have an important meaning as marking. In Experiment 3, marking and marking-like behavior in castrated adult Mongolian gerbils aged 16 weeks were observed. The result showed that marking behavior, not marking-like behavior was inhibited after castration. From these findings, we consider that generally marking behavior in Mongolian gerbils consists of androgen-dependent marking behavior and androgen-independent marking behavior (marking-like behavior). 相似文献
55.
Koshizuka Y Yamada T Hoshi K Ogasawara T Chung UI Kawano H Nakamura Y Nakamura K Ikegawa S Kawaguchi H 《The Journal of biological chemistry》2003,278(48):48259-48266
This study attempts to characterize cystatin 10 (Cst10), which we recently identified as a novel protein implicated in endochondral ossification. Expression of Cst10 was specific to cartilage, localized in the cytosol of prehypertrophic and hypertrophic chondrocytes of the mouse growth plate. In the mouse chondrogenic cell line ATDC5, Cst10 expression preceded type X collagen expression and increased in synchrony with maturation. When we compared ATDC5 cells transfected with Cst10 cDNA with cells transfected with a mock vector, hypertrophic maturation and mineralization of chondrocytes were promoted by Cst10 gene overexpression in that type X collagen expression was observed earlier, and alizarin red staining was stronger. On the other hand, type II collagen expression and Alcian blue staining, both of which are markers of the early stage of chondrocyte differentiation, were similar in both cells. Overexpression of the Cst10 gene also caused fragmentation of nuclei, the appearance of annexin V, a change in the mitochondrial membrane potential, and activation of caspases. These results strongly suggest that Cst10 may play an important role in the last steps of the chondrocyte differentiation pathway as an inducer of maturation, followed by apoptosis of chondrocytes. 相似文献
56.
Okada Y Makino S Tobe T Okada N Yamazaki S 《Applied and environmental microbiology》2002,68(4):1541-1547
Transposon insertional mutants of Listeria monocytogenes were constructed to identify genes involved in osmotolerance, and one mutant that showed reduced growth under high osmotic pressure was obtained. The cloned gene from the transposon insertion site of the mutant, named rel, was 2,214 bp in length and had very high homology to relA of Bacillus subtilis, which encodes guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp) [collectively designated (p)ppGpp] synthetase during stringent response. The mutant showed a deficiency in (p)ppGpp accumulation. In the parental strain, the amount of intracellular (p)ppGpp was not increased after an osmotic upshift but was slightly decreased compared with the level before the upward shift. The reduced osmotolerance of the mutant was restored to a level almost equal to that of the parent strain when the chromosomal region that included rel of L. monocytogenes was introduced into the mutant. After exposure to methyl glucoside, the rel mutant accumulated (p)ppGpp at a higher level than the basal level and partially restored the ability to grow in NaCl-supplemented brain heart infusion broth. The mutant was found to grow in chemically defined minimal medium supplemented with glycine betaine or carnitine, so-called compatible solutes, and 4% NaCl. Our results suggest that the appropriate intracellular concentration of (p)ppGpp is essential for full osmotolerance in L. monocytogenes and that its mechanism is different from that for the accumulation of compatible solutes. 相似文献
57.
Regulation of angiogenesis by the aging suppressor gene klotho 总被引:5,自引:0,他引:5
Fukino K Suzuki T Saito Y Shindo T Amaki T Kurabayashi M Nagai R 《Biochemical and biophysical research communications》2002,293(1):332-337
Advanced age is a major risk factor of peripheral artery disease. We examined the effects of the aging-suppressor gene klotho on angiogenesis in response to ischemia by introducing ischemic hindlimb model in mice heterozygously deficient for the klotho gene and in wild type mice. Blood flow recovery as assessed by laser doppler perfusion imaging and angiogenesis as assessed by density of PECAM-1/CD31-positive positive capillaries were markedly impaired in mice heterozygously deficient for the klotho gene (both <0.05). Our findings show that the aging-suppressor gene klotho affects angiogenesis and the possibility that age-related impairment of angiogenesis might be regulated by the klotho gene. Our results present a new possibility of therapeutic angiogenesis for patients of advanced age. 相似文献
58.
Dear Editor:
Geldanamycin is a benzoquinone ansamycin, which was originally described as a tyrosine kinase inhibitor. However, subsequent studies have revealed that geldanamycin binds to and inhibits heat-shock protein 90 (Hsp90) activity [1]. Hsp90 is a molecular chaperone involved in the conformational maturation of proteins such as mutated p53, Raf- 1, Akt, Bcr-Abl, and ErbB2. It is suggested that agents inhibiting Hsp90 have anti-cancer properties, although the precise molecular mechanisms underlying the anti-cancer effects of geldanamycin are not well understood. 相似文献
59.
Shigeru Kitanishi Toshiaki Yamamoto Hiromi Ishii Yuko Yamaguchi Toru Kobayashi 《Ichthyological Research》2017,64(1):111-115
To evaluate the influences of spatial scale on dispersal, the dispersal patterns of masu salmon Oncorhynchus masou masou were investigated at among-river (ca. <43 km) and within-river levels in mid-western Hokkaido, Japan. A genetic differentiation (F ST) and assignment test showed that among-river dispersal was much less common (2.9 % of 339 individuals) than within-river dispersal (7.4 % of 190 individuals). We also found that there was no bias in dispersal at the among-river level, while anadromous males were more likely to disperse at the within-river level, suggesting that the dispersal patterns may be scale dependent. 相似文献
60.
Harauma A Murayama T Ikeyama K Sano H Arai H Takano R Kita T Hara S Kamei K Yokode M 《Biochemical and biophysical research communications》2007,358(3):751-756
Mulberry is commonly used to feed silkworms. Here we examined whether a dietary intake of mulberry leaf (ML) could affect atherogenesis in vivo and in vitro. Apolipoprotein E-deficient mice were fed either normal chow (control group) or a diet containing 1% ML powder (ML group) from 6 weeks of age. The mice were sacrificed after 12 weeks. The susceptibility of plasma lipoprotein to oxidation was assessed using diene formation. A significant increase in the lag time of lipoprotein oxidation was detected in the ML group compared with the control group. Furthermore, the ML group showed a 40% reduction in atherosclerotic lesion size in the aortae compared with the control. We also examined the direct anti-oxidative activity of ML in vitro. Aqueous extract of ML had a strong scavenging effect on 1,1-diphenyl-2-picrylhydrazyl and inhibited lipoprotein oxidation. These results confirm that ML contains anti-oxidative substances that might help prevent atherosclerosis. 相似文献