全文获取类型
收费全文 | 760篇 |
免费 | 55篇 |
专业分类
815篇 |
出版年
2023年 | 7篇 |
2022年 | 4篇 |
2021年 | 14篇 |
2020年 | 7篇 |
2019年 | 7篇 |
2018年 | 8篇 |
2017年 | 17篇 |
2016年 | 27篇 |
2015年 | 36篇 |
2014年 | 48篇 |
2013年 | 53篇 |
2012年 | 44篇 |
2011年 | 52篇 |
2010年 | 32篇 |
2009年 | 35篇 |
2008年 | 47篇 |
2007年 | 50篇 |
2006年 | 32篇 |
2005年 | 43篇 |
2004年 | 31篇 |
2003年 | 38篇 |
2002年 | 38篇 |
2001年 | 9篇 |
2000年 | 8篇 |
1999年 | 2篇 |
1998年 | 8篇 |
1997年 | 8篇 |
1996年 | 7篇 |
1995年 | 6篇 |
1994年 | 6篇 |
1993年 | 9篇 |
1992年 | 12篇 |
1991年 | 6篇 |
1990年 | 2篇 |
1989年 | 3篇 |
1988年 | 5篇 |
1987年 | 2篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1982年 | 7篇 |
1981年 | 9篇 |
1980年 | 7篇 |
1979年 | 4篇 |
1978年 | 3篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1974年 | 2篇 |
1970年 | 3篇 |
1967年 | 1篇 |
1963年 | 1篇 |
排序方式: 共有815条查询结果,搜索用时 0 毫秒
791.
792.
793.
794.
795.
Cooling is an efficient method to immobilise insects for handling, but it is known that fast cooling to temperatures above
freezing can cause chill injury and/or death. The mortality of Asian weaver ants, Oecophylla smaragdina, a tropical species that does not normally encounter near freezing temperatures, was investigated in experiments with cooling
temperatures of 0 and 5°C and duration of cooling periods of 45 and 150 min. No significant difference in mortality was found
between ants cooled to 5 and 0°C; however, cooling to these temperatures led to higher mortalities compared to non-cooled
control ants. Following both 45 and 150 min of cold treatment, a significant excessive mortality of cooled ants (compared
to uncooled ants) was observed, indicating that effects of cooling on mortality should be considered when performing studies
on Asian weaver ants. 相似文献
796.
Metagenomics facilitates the study of the genetic information from uncultured microbes and complex microbial communities. Assembling complete genomes from metagenomics data is difficult because most samples have high organismal complexity and strain diversity. Some studies have attempted to extract complete bacterial, archaeal, and viral genomes and often focus on species with circular genomes so they can help confirm completeness with circularity. However, less than 100 circularized bacterial and archaeal genomes have been assembled and published from metagenomics data despite the thousands of datasets that are available. Circularized genomes are important for (1) building a reference collection as scaffolds for future assemblies, (2) providing complete gene content of a genome, (3) confirming little or no contamination of a genome, (4) studying the genomic context and synteny of genes, and (5) linking protein coding genes to ribosomal RNA genes to aid metabolic inference in 16S rRNA gene sequencing studies. We developed a semi-automated method called Jorg to help circularize small bacterial, archaeal, and viral genomes using iterative assembly, binning, and read mapping. In addition, this method exposes potential misassemblies from k-mer based assemblies. We chose species of the Candidate Phyla Radiation (CPR) to focus our initial efforts because they have small genomes and are only known to have one ribosomal RNA operon. In addition to 34 circular CPR genomes, we present one circular Margulisbacteria genome, one circular Chloroflexi genome, and two circular megaphage genomes from 19 public and published datasets. We demonstrate findings that would likely be difficult without circularizing genomes, including that ribosomal genes are likely not operonic in the majority of CPR, and that some CPR harbor diverged forms of RNase P RNA. Code and a tutorial for this method is available at https://github.com/lmlui/Jorg and is available on the DOE Systems Biology KnowledgeBase as a beta app. 相似文献
797.
Diminished nuclear RNA decay upon Salmonella infection upregulates antibacterial noncoding RNAs
下载免费PDF全文
![点击此处可从《The EMBO journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Katsutoshi Imamura Akiko Takaya Yo‐ichi Ishida Yayoi Fukuoka Toshiki Taya Ryo Nakaki Miho Kakeda Naoto Imamachi Aiko Sato Toshimichi Yamada Rena Onoguchi‐Mizutani Gen Akizuki Tanzina Tanu Kazuyuki Tao Sotaro Miyao Yutaka Suzuki Masami Nagahama Tomoko Yamamoto Torben Heick Jensen Nobuyoshi Akimitsu 《The EMBO journal》2018,37(13)
798.
The MHC class I gene family of rhesus macaques is characterised by considerable gene duplications. While a HLA-C-orthologous gene is absent, the Mamu-A and in particular the Mamu-B genes have expanded, giving rise to plastic haplotypes with differential gene content. Although some of the rhesus macaque
MHC class I genes are known to be associated with susceptibility/resistance to infectious diseases, the functional significance of duplicated
Mamu-A and Mamu-B genes and the expression pattern of their encoded proteins are largely unknown. Here, we present data of the subcellular
localization of AcGFP-tagged Mamu-A and Mamu-B molecules. We found strong cell surface and low intracellular expression for
Mamu-A1, Mamu-A2 and Mamu-A3-encoded molecules as well as for Mamu-B*01704, Mamu-B*02101, Mamu-B*04801, Mamu-B*06002 and Mamu-B*13401. In contrast, weak
cell surface and strong intracellular expression was seen for Mamu-A4*1403, Mamu-B*01202, Mamu-B*02804, Mamu-B*03002, Mamu-B*05704,
Mamu-I*010201 and Mamu-I*0121. The different expression patterns were assigned to the antigen-binding α1 and α2 domains, suggesting
failure of peptide binding is responsible for retaining ‘intracellular’ Mamu class I molecules in the endoplasmic reticulum.
These findings indicate a diverse functional role of the duplicated rhesus macaque MHC class I genes. 相似文献
799.
800.
Manfred Heinlem Torben Brattig Reinhard Kunze 《The Plant journal : for cell and molecular biology》1994,5(5):705-714
The transposase (TPase) of the maize transposon Activator (Ac) accumulates in the nuclei of maize endosperm and transfected Petunia protoplasts, where it aggregates into rod-like structures about 2 μm in length. In petunia protoplasts the amount of TPase aggregates increases with the strength of the promoter fused to the Ac-coding region. The excision frequency of a Ds element, however, does not increase proportionally. The data suggest that the aggregated TPase is not responsible for the mobilization of the Ds element, but rather is a transpositionally inactive form of the protein. In contrast to the full-length TPase, a functional, N-terminally truncated TPase derivative is inefficiently transported into the nucleus at high expression levels and aggregates predominantly in the cytoplasm. Accordingly, the N-terminus of the TPase is involved in nuclear localization and/or aggregation. 相似文献