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971.
Tomoyuki Tanaka Nana Yajima Tomoko Kiyoshi Yoshiki Miura Seiji Iwama 《Bioorganic & medicinal chemistry letters》2017,27(1):94-97
Optimization of the previously reported benzothiazine analogue A led to the identification of compound 1, which showed anti-convulsant activity in two golden standard animal models of seizure, the MES and scPTZ models. Structure-activity relationship investigation of compound 1 revealed compounds 2, 6 and 19 as attractive anti-epileptic drug (AED) candidates with potent anticonvulsant effect in both the MES and scPTZ models. As these compounds are structurally different from existing AEDs, determination of their mechanism of actions could provide clues to understanding current therapy-resistant seizures. Moreover, these simple phenylsulfoneamide compounds could be good starting points for searching broad spectrum AEDs by such in vivo screening. 相似文献
972.
973.
Noriko Okazaki Reiko Kikuno Reiko Ohara Susumu Inamoto Hidenori Aizawa Shigeki Yuasa Daisuke Nakajima Takahiro Nagase Osamu Ohara Hisashi Koga 《DNA research》2003,10(1):35-48
We have accumulated information of the coding sequences of uncharacterized human genes, which are known as KIAA genes, and the number of these genes exceeds 2000 at present. As an extension of this sequencing project, we recently have begun to accumulate mouse KIAA-homologous cDNAs, because it would be useful to prepare a set of human and mouse homologous cDNA pairs for further functional analysis of the KIAA genes. We herein present the entire sequences of 400 mouse KIAA cDNA clones and 4 novel cDNA clones which were incidentally identified during this project. Most of clones entirely sequenced in this study were selected by computer-assisted analysis of terminal sequences of the cDNAs. The average size of the 404 cDNA sequences reached 5.3 kb and that of the deduced amino acid sequences from these cDNAs was 868 amino acid residues. The results of sequence analyses of these clones showed that single mouse KIAA cDNAs bridged two different human KIAA cDNAs in some cases, which indicated that these two human KIAA cDNAs were derived from single genes although they had been supposed to originate from different genes. Furthermore, we successfully mapped all the mouse KIAA cDNAs along the genome using a recently published mouse genome draft sequence. 相似文献
974.
The burrow defense behaviors in a sand-bubbler crab, Scopimera globosa, living on a tidal flat, were experimentally examined. Body size and prior residence influenced the results of struggles
for the burrows, and large individuals or the burrow owners won in most cases when the intruders were not significantly larger
than the owners. Most large owners defended their burrows by directly fighting their opponents. On the other hand, small owners
defended their burrows in three different ways. (1) Owners fought directly against same-sized or smaller intruders. For larger
intruders, (2) most owners returned to their burrows when the owner was nearer to the burrow than the intruder (returning
behavior), and (3) owners sat motionless when the intruder was nearer to the burrow than the owner (sitting behavior). Success
ratios of the three types of burrow defense were 38.2%, 88.5%, and 100%, respectively. It was considered that sitting behavior
of the cryptically colored S. globosa has evolved because intruders cannot see motionless owners and consequently cannot detect the owner's burrow.
Received: October 6, 2000 / Accepted: January 22, 2001 相似文献
975.
The relationship between fig trees and their pollinator wasps is a well-known example of species-specific obligate mutualism.
In this article we present a stochastic model of this mutualistic system, referring to data on a dioecious fig (Ficus schwarzii) in Borneo, and examine the conditions for the persistence of a wasp population for a given period. (1) When the average
duration of the flowering interval of fig trees is short, even a small fig population can sustain a wasp population successfully.
A population whose average period of flowering cycle is half that of another population can sustain a wasp population with
a number of trees less than half of the other population. (2) The wasp survival rate (WSR) is higher when (a) the variation
of the interval periods of fig flowering is smaller, (b) the fig population size is larger, and (c) figs can prolong their
receptivity to wait for a wasp if no wasps are available. (3) WSR is predictable from the average proportion of the fig's
receptive phases, in which wasps are available, to their total receptive phases. (4) The persistence period of a wasp population
increases exponentially with the number of fig trees. Based on these results we propose a new hypothesis, as a possible scenario,
on the evolution of dioecy from monoecy in Ficus.
Received: November 13, 1998 / Accepted: July 14, 1999 相似文献
976.
The imprinting behavior of chicks was quantified as a preference score (correct response ratio) achieved in a running wheel apparatus. A total of 249 chicks were exposed to an imprinting stimulus and tested for stimulus-approaching behavior. The chicks were then classified as good learners (imprinted), poor learners (non-imprinted) and a gray-zone group, those were 46%, 31% and 23% of the total chicks respectively. Using the classified chicks, the acetylcholine (ACh) and glutamate releases from the medial hyperstriatum ventrale (MHV) of the chick forebrains were determined by in vivo microdialysis. The non-imprinted chicks were used as yoked controls. Increases of ACh and glutamate released were observed in the imprinted chicks during exposure to the imprinting stimulus, whereas there were no changes in the release of these neurotransmitters in the non-imprinted chicks during the imprinting exposure. These results might be indicated that cholinergic and glutamatergic synapses which are newly formed as functioning synapses with imprinting stimulus in the MHV are involved in the performance of imprinting behavior. 相似文献
977.
E Suematsu M Hirata J Nishimura T Koga H Ibayashi 《Archives of biochemistry and biophysics》1987,257(2):315-320
The effects of guanosine triphosphate (GTP) on the release and uptake of Ca2+ in nonmitochondrial intracellular store sites of human peripheral lymphocytes were examined. GTP in the presence of 3% polyethylene glycol released Ca2+ from the intracellular store sites of lymphocytes in a dose-dependent manner, and the maximal release was obtained at 10 microM GTP. GDP and 5'-GMP also enhanced the release of Ca2+. On the other hand, Ca2+ uptake in the presence of oxalate by saponin-treated lymphocytes was stimulated by GTP and this stimulation was abolished when polyethylene glycol was concomitantly present. The dose dependence of the stimulated Ca2+ uptake by GTP was much the same as that of the Ca2+ released by GTP. These results indicate that GTP has an inherent activity to release Ca2+ as well as to stimulate the uptake of Ca2+ in nonmitochondrial intracellular store sites of saponin-treated lymphocytes. The stimulatory effect of polyethylene glycol on GTP-mediated Ca2+ release may occur by inhibiting functions of the Ca2+ pump. 相似文献
978.
979.
Shimoji T Kanda H Kitagawa T Kadota K Asai R Takahashi K Kawaguchi N Matsumoto S Hayashizaki Y Okazaki Y Shinomiya K 《Biochemical and biophysical research communications》2004,314(4):1133-1140
Well-differentiated liposarcoma (WD) acquires fully malignant potential when the histological progression named dedifferentiation occurs. This progression is supposed to occur in a time-dependent manner but this is still a debated issue. Clinically, the prediction of dedifferentiation for WD is very important from the therapeutic point of view. To identify genes that are predictive of dedifferentiation and to understand the mechanism of dedifferentiation, we investigated clinical information of 50 cases and studied the gene expression profiles of 36 lipomatous tumors using cDNA microarray. The clinical study showed that the dedifferentiation did not always seem to occur in a time-dependent manner. Interestingly, from the gene expression study, unsupervised hierarchical clustering analysis of well-differentiated lesions obtained from dedifferentiated liposarcoma (DD) cases that were indistinguishable from WD pathologically showed a clearly distinct gene expression pattern from WD. Using the pattern-matching program, 1687 genes including 487 known genes were identified, which discriminated WD cases from well-differentiated lipomatous lesions obtained from DD cases. These results suggest that the dedifferentiation may arise from different types of WD that could be distinguished from gene expression profiling but could hardly be classified by the pathological studies. 相似文献
980.
Molecular cloning of endo-beta-D-1,4-glucanase genes,rce1, rce2, and rce3, from Rhizopus oryzae
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Moriya T Murashima K Nakane A Yanai K Sumida N Koga J Murakami T Kono T 《Journal of bacteriology》2003,185(5):1749-1756
Three endoglucanase genes, designated the rce1, rce2, and rce3 genes, were isolated from Rhizopus oryzae as the first cellulase genes from the subdivision ZYGOMYCOTA: All the amino acid sequences deduced from the rce1, rce2, and rce3 genes consisted of three distinct domains: cellulose binding domains, linker domains, and catalytic domains belonging to glycosyl hydrolase family 45. The rce3 gene had two tandem repeated sequences of cellulose binding domains, while rce1 and rce2 had only one. rce1, rce2, and rce3 had various lengths of linker sequences. 相似文献