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191.
The conditions required for the electroporation of a plasmidwhich harbored the gene for chloramphenicol acetyltransferase(CAT) into protoplasts from cultivated tomato leaves were optimizedat 666 or 7,000 V/cm (single discharge) using a 47-µFcapacitor in the presence of 10-100 µg DNA. The CAT genewas strongly expressed by when under the control of the promoterfor the 35S RNA from cauliflower mosaic virus and CAT activitywas detected in cells 10 days after electroporation. (Received July 28, 1988; Accepted March 23, 1989) 相似文献
192.
Tomonobu Hasegawa Tsutomu Ogata Yukihiro Hasegawa Masataka Honda Toshiro Nagai Yoshimitsu Fukushima Yutaka Nakahori Nobutake Matsuo 《Human genetics》1996,97(5):564-567
This paper describes a 12-year-old Japanese boy with coarctation of the aorta, renal hypoplasia, Turner/ Noonan surface anomalies,
and a 46,XY karyotype. Although the patient might represent an exceptional case of Noonan syndrome, the combination of the
somatic stigmata appears to be consistent with a mutation of the putative lymphogenic gene(s) for Turner somatic stigmata.
Received: 24 June 1995 / Revised: 28 September 1995 相似文献
193.
G. H. M. Sagor Stefan Simm Dong Wook Kim Masaru Niitsu Tomonobu Kusano Thomas Berberich 《Physiology and Molecular Biology of Plants》2021,27(3):577
Arabidopsis thaliana polyamine oxidase 5 gene (AtPAO5) functions as a thermospermine (T-Spm) oxidase. Aerial growth of its knock-out mutant (Atpao5-2) was significantly repressed by low dose(s) of T-Spm but not by other polyamines. To figure out the underlying mechanism, massive analysis of 3′-cDNA ends was performed. Low dose of T-Spm treatment modulates more than two fold expression 1,398 genes in WT compared to 3186 genes in Atpao5-2. Cell wall, lipid and secondary metabolisms were dramatically affected in low dose T-Spm-treated Atpao5-2, in comparison to other pathways such as TCA cycle-, amino acid- metabolisms and photosynthesis. The cell wall pectin metabolism, cell wall proteins and degradation process were highly modulated. Intriguingly Fe-deficiency responsive genes and drought stress-induced genes were also up-regulated, suggesting the importance of thermospermi′ne flux on regulation of gene network. Histological observation showed that the vascular system of the joint part between stem and leaves was structurally dissociated, indicating its involvement in vascular maintenance. Endogenous increase in T-Spm and reduction in H2O2 contents were found in mutant grown in T-Spm containing media. The results indicate that T-Spm homeostasis by a fine tuned balance of its synthesis and catabolism is important for maintaining gene regulation network and the vascular system in plants. 相似文献
194.
Chika Tateda Koji Yamashita Fumio Takahashi Tomonobu Kusano Yoshihiro Takahashi 《Plant cell reports》2009,28(7):1145-1145