全文获取类型
收费全文 | 2781篇 |
免费 | 176篇 |
专业分类
2957篇 |
出版年
2022年 | 24篇 |
2021年 | 27篇 |
2020年 | 21篇 |
2019年 | 40篇 |
2018年 | 38篇 |
2017年 | 47篇 |
2016年 | 61篇 |
2015年 | 103篇 |
2014年 | 100篇 |
2013年 | 166篇 |
2012年 | 215篇 |
2011年 | 180篇 |
2010年 | 132篇 |
2009年 | 112篇 |
2008年 | 192篇 |
2007年 | 222篇 |
2006年 | 192篇 |
2005年 | 172篇 |
2004年 | 186篇 |
2003年 | 178篇 |
2002年 | 177篇 |
2001年 | 27篇 |
2000年 | 20篇 |
1999年 | 27篇 |
1998年 | 31篇 |
1997年 | 20篇 |
1996年 | 25篇 |
1995年 | 20篇 |
1994年 | 24篇 |
1993年 | 20篇 |
1992年 | 9篇 |
1991年 | 14篇 |
1990年 | 8篇 |
1989年 | 7篇 |
1988年 | 6篇 |
1987年 | 13篇 |
1986年 | 6篇 |
1985年 | 9篇 |
1984年 | 14篇 |
1983年 | 9篇 |
1982年 | 7篇 |
1981年 | 5篇 |
1980年 | 5篇 |
1976年 | 4篇 |
1975年 | 5篇 |
1974年 | 3篇 |
1973年 | 6篇 |
1972年 | 5篇 |
1971年 | 4篇 |
1969年 | 3篇 |
排序方式: 共有2957条查询结果,搜索用时 15 毫秒
21.
22.
23.
Yan G. Ni Jon H. Condra Laura Orsatti Xun Shen Stefania Di Marco Shilpa Pandit Matthew J. Bottomley Lionello Ruggeri Richard T. Cummings Rose M. Cubbon Joseph C. Santoro Anka Ehrhardt Dale Lewis Timothy S. Fisher Sookhee Ha Leila Njimoluh Dana D. Wood Holly A. Hammond Douglas Wisniewski Cinzia Volpari Alessia Noto Paola Lo Surdo Brian Hubbard Andrea Carf�� Ayesha Sitlani 《The Journal of biological chemistry》2010,285(17):12882-12891
24.
Nakashima A Maruki Y Imamura Y Kondo C Kawamata T Kawanishi I Takata H Matsuura A Lee KS Kikkawa U Ohsumi Y Yonezawa K Kamada Y 《PloS one》2008,3(5):e2223
The target of rapamycin (Tor) protein plays central roles in cell growth. Rapamycin inhibits cell growth and promotes cell cycle arrest at G1 (G0). However, little is known about whether Tor is involved in other stages of the cell division cycle. Here we report that the rapamycin-sensitive Tor complex 1 (TORC1) is involved in G2/M transition in S. cerevisiae. Strains carrying a temperature-sensitive allele of KOG1 (kog1-105) encoding an essential component of TORC1, as well as yeast cell treated with rapamycin show mitotic delay with prolonged G2. Overexpression of Cdc5, the yeast polo-like kinase, rescues the growth defect of kog1-105, and in turn, Cdc5 activity is attenuated in kog1-105 cells. The TORC1-Type2A phosphatase pathway mediates nucleocytoplasmic transport of Cdc5, which is prerequisite for its proper localization and function. The C-terminal polo-box domain of Cdc5 has an inhibitory role in nuclear translocation. Taken together, our results indicate a novel function of Tor in the regulation of cell cycle and proliferation. 相似文献
25.
Production of Reactive Oxygen Species and Release of l-Glutamate During Superoxide Anion-Induced Cell Death of Cerebellar Granule Neurons 总被引:3,自引:0,他引:3
Takumi Satoh Tadahiro Numakawa Yasuhiro Abiru Tomoko Yamagata Yasuyuki Ishikawa Yasushi Enokido Hiroshi Hatanaka 《Journal of neurochemistry》1998,70(1):316-324
Abstract: Enhanced production of superoxide anion (O2 − ) is considered to play a pivotal role in the pathogenesis of CNS neurons. Here, we report that O2 − generated by xanthine (XA) + xanthine oxidase (XO) triggered cell death associated with nuclear condensation and DNA fragmentation in cerebellar granule neuron. XA + XO induced significant increases in amounts of intracellular reactive oxygen species (ROS) before initiating loss of cell viability, as determined by measurement of 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) (C-DCDHF-DA) for O2 − and other ROS and hydroethidine (HEt) specifically for O2 − by using fluorescence microscopy and flow cytometry. Catalase, but not superoxide dismutase (SOD), significantly protected granule neurons from the XA + XO-induced cell death. Catalase effectively reduced C-DCDHF-DA but not HEt fluorescence, whereas SOD reduced HEt but not C-DCDHF-DA fluorescence, indicating that HEt and C-DCDHF-DA fluorescence correlated with O2 − and hydrogen peroxide, respectively. The NMDA antagonist MK-801 prevented the death. XA + XO induced an increase in l -glutamate release from cerebellar granule neurons. These results indicate that elevation of O2 − induces cell death associated with increasing ROS production in cerebellar granule neurons and that XA + XO enhanced release of l -glutamate. 相似文献
26.
27.
Chloroplast-nucleus interactions were examined in cells of Euglenagracilis Z synchronized under photoautotrophic conditions. Thechloroplasts were localized near the cell periphery. At an earlystage of the cell cycle, however, some chloroplasts were transientlylocated in the inner space close to the nucleus. Electron microscopyusing serial cell sections revealed that the chloroplast formedprotrusions at several sites, which became associated with thenucleus. The outer membrane of the chloroplast envelope wasin contact, or at least continuous in part, with the outer membraneof the nuclear envelope at the sites of association, and densematerial was present in the chloroplast membrane. A chromosomewas close to each site of the association between these twoorganelles. Most of the chloroplasts including those in associationwith the nucleus were connected by fine bridges. The 4',6-diamidino-2-phenylindole-stainednucleoids in the chloroplast associated with the nucleus appearedto have a thread-like shape. There was another type of chloroplast-nucleusconnection, in which an intervening membranous body was in contactwith the outer part of the nuclear envelope on one side andwith the chloroplast envelope on the other side.
1 This work was reported at the 48th Annual Meeting of the BotanicalSociety of Japan, Kyoto, October, 1983. (Received June 5, 1984; Accepted November 20, 1984) 相似文献
28.
29.
Takimoto Yuki Monkawa Akira Nagata Kohki Kobayashi Masahiro Kinoshita Mariko Gessei Tomoko Mori Toshiya Kagi Hiroyuki 《Plasmonics (Norwell, Mass.)》2020,15(3):805-811
Plasmonics - Detection and monitoring of SO2 is important because it is a representative toxic gas in the atmospheric environment that is emitted from industrial and natural processes. Localized... 相似文献