全文获取类型
收费全文 | 1904篇 |
免费 | 169篇 |
出版年
2022年 | 10篇 |
2021年 | 27篇 |
2020年 | 11篇 |
2019年 | 15篇 |
2018年 | 23篇 |
2017年 | 19篇 |
2016年 | 43篇 |
2015年 | 71篇 |
2014年 | 55篇 |
2013年 | 118篇 |
2012年 | 135篇 |
2011年 | 116篇 |
2010年 | 78篇 |
2009年 | 75篇 |
2008年 | 120篇 |
2007年 | 106篇 |
2006年 | 96篇 |
2005年 | 108篇 |
2004年 | 138篇 |
2003年 | 102篇 |
2002年 | 87篇 |
2001年 | 46篇 |
2000年 | 51篇 |
1999年 | 47篇 |
1998年 | 30篇 |
1997年 | 21篇 |
1996年 | 19篇 |
1995年 | 25篇 |
1994年 | 17篇 |
1993年 | 12篇 |
1992年 | 22篇 |
1991年 | 23篇 |
1990年 | 24篇 |
1989年 | 19篇 |
1988年 | 14篇 |
1987年 | 14篇 |
1986年 | 25篇 |
1985年 | 13篇 |
1984年 | 18篇 |
1983年 | 11篇 |
1982年 | 8篇 |
1981年 | 7篇 |
1980年 | 4篇 |
1979年 | 12篇 |
1978年 | 8篇 |
1976年 | 3篇 |
1975年 | 3篇 |
1972年 | 5篇 |
1968年 | 3篇 |
1967年 | 3篇 |
排序方式: 共有2073条查询结果,搜索用时 15 毫秒
11.
Y Hashimoto K Chida M Huang M Katayama T Nishihira T Kuroki 《Biochemical and biophysical research communications》1989,163(1):406-411
The protein kinase C (PKC) activities of tumor tissue and adjacent normal mucosa of human cancers of the esophagus (8 cases), stomach (1 case) and colon (3 cases) were measured. Considerable variations were found in the activity of PKC and in its subcellular distribution in these cancers. The PKC activities of the membrane and cytosolic fractions of the eight esophageal cancers were, however, similar to those of the adjacent normal mucosa: the average PKC activities of the tumor tissues and normal mucosa were 7.5 and 8.3 pmol/min/mg protein, respectively, in their membrane fractions and 7.9 and 7.8 pmol/min/mg protein, respectively, in their cytosolic fractions. 相似文献
12.
13.
S Katayama M Inaba Y Maruno A Omoto S Kawazu J Ishii M Sawada 《Prostaglandins, leukotrienes, and essential fatty acids》1990,39(1):47-51
The present study was designed to determine urinary excretion of kallikrein(KAL)-kinin as well as prostaglandin (PG) E2, TXB2 and 2,3-dinor-TXB2, a major urinary metabolite of TXA2 synthesized in platelets, by specific RIAs in patients with diabetes mellitus (DM). KAL or kinin excretion in 26 type II DM did not differ from control values obtained in 18 age-matched healthy subjects (C), although DM with HbA1 greater than 11% excreted less KAL. Urinary PGE2 excretion (7.6 +/- 2.8 ng/mg creatinine, mean +/- SE) was significantly lower in DM compared to C (17.5 +/- 3.9, p less than 0.05), while DM excreted more TXB2 (0.57 +/- 0.09, p less than 0.01) and 2,3-dinor-TXB2 (0.56 +/- 0.12, N.S.) than C (0.19 +/- 0.02 or 0.33 +/- 0.01). DM with or without mild proteinuria demonstrated lower PGE2, but higher TXB2 and 2,3-dinor-TXB2 excretion. A positive correlation of TXB2/2,3-dinor-TXB2 with proteinuria was observed in this group. However, in DM with massive proteinuria over 500 micrograms/mg creatinine, TXB2 and 2,3-dinor-TXB2 excretion decreased to levels almost identical to C. As a whole, a ratio of TXB2 to PGE2 or 2,3-dinor-TXB2 in DM was significantly higher than in C. The results suggest that a relative preponderance of TXB2 to 2,3-dinor-TXB2 may indicate an augmented renal, in addition to platelet, TXA2 synthesis. An excessive vasoconstrictive and proaggregatory TXA2 renal synthesis, concomitant with a decrease in vasodilatory and antiaggregatory PGE2, may have profound effects on renal functions such as protein excretion in DM. 相似文献
14.
K Mizukawa T Ogura I Yamamoto T Mitsui E Katayama Z Ota N Ogawa 《Regulatory peptides》1988,21(1-2):167-172
The localization of receptors for atrial natriuretic polypeptide (ANP) in the glomerulus of the rat was studied by electron microscopic autoradiography using 125I-labeled ANP. A total of 1,134 silver grains counted in 30 glomeruli were distributed as follows: processes of the podocytes (36.4%), cell bodies of the podocytes (14.8%), basement membrane (12.3%), endothelial cells (6.3%), mesangial cells (7.7%) and others, for example the vascular spaces (22.5%). This finding indicates that ANP binding sites are mainly localized on the foot processes of the podocytes in the glomerulus. 相似文献
15.
Tomohiko Suzuki Akio Nakamura Yoshiaki Satoh Chisato Inai Takahiro Furukohri Tomikazu Arita 《Journal of Protein Chemistry》1992,11(6):629-633
The blood clamBarbatia virescens has a heterodimeric hemoglobin in erythrocytes. Interestingly, the congeneric clamsB. reeveana andB. lima contain quite different hemoglobins: tetramer and polymeric hemoglobin consisting of unusual didomain chain. The complete amino acid sequence of chain I ofB. virescens has been determined. The sequence was mainly determined from CNBr peptides and their subpeptides, and the alignment of the peptides was confirmed by sequencing of PCR-amplified cDNA forB. virescens chain I. The cDNA-derived amino acid sequence matched completely with the sequence proposed from protein sequencing.B. virescens chain I is composed of 156 amino acid residues, and the molecular mass was calculated to be 18,387 D, including a heme group. The sequence ofB. virescens chain I showed 35–42% sequence identity with those of the related clamAnadara trapezia and the congeneric clamB. reeveana. An evolutionary tree forAnadara andBarbatia chains clearly indicates that all of the chains are evolved from one ancestral globin gene, and that the divergence of chains has occurred in each clam after the speciation. The evolutionary rate for clam hemoglobins was estimated to be about four times faster than that of vertebrate hemoglobin. We suggest that blood clam hemoglobin is a physiologically less important molecule when compared with vertebrate hemoglobins, and so it evolved rapidly and resulted in a remarkable diversity in quaternary and subunit structure within a relatively short period. 相似文献
16.
17.
The nucleotide sequence of a 4.2-kb EcoRI fragment from the intergenic region between the genes for 25S and 18S ribosomal RNA of Pharbitis nil Choisy was determined. The region contained a unique repetitive family of DNA sequences, called the RsaI family, composed of 32-bp units. The 32-bp unit was tandemly repeated in the intergenic region, and four subfamilies of repeating units were clustered as discrete blocks. The RsaI family of repeats was shown to be specific to the genus Pharbitis by Southern blot hybridization. 相似文献
18.
The N-terminal amino acid sequences of human urinary prokallikrein and kallikrein have been determined. Their amino acid sequences are as follows. (Formula; see text) The results showed that prokallikrein comprises an additional seven amino acids at the amino terminus of the kallikrein, of which the sequence is (H2N)Ala-Pro-Pro-Ile-Gln-Ser-Arg(COOH). Comparison of the structure of this peptide with those of other proteins revealed extensive sequence identity with the propeptide portions of rat and mouse tissue kallikreins, that were predicted from the preproenzyme-encoded nucleotide sequences. The amino acid sequence of the peptide was also highly homologous to that of the propeptide portion of EGF-binding protein, that was predicted from the nucleotide sequence, and that of the alpha-subunit of NGF. The N-terminal amino acid sequence of kallikrein was completely identical to the reported one (Lottspeich, F., et al. (1979) Hoppe-Seyler's Z. Physiol. Chem. 360, 1947-1950) and shows considerable amino acid sequence homology with the porcine and rat pancreatic kallikreins. As far as the present results are concerned, it is strongly indicated that the inactive kallikrein in human urine is a tissue type prokallikrein which is activated on the release of the N-terminal peptide consisting of seven amino acids. 相似文献
19.
Spiral conformation of Vibrio cholerae as determined by scanning electron microscopy of elongated cells induced by cephalexin treatment. 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Journal of bacteriology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The elongated cells of Vibrio spp. induced by cephalexin treatment were examined by scanning electron microscopy. The results showed that Vibrio cholerae has a twisted cell body and a right-handed spiral conformation and that the cell bodies of V. parahaemolyticus and V. alginolyticus are straight rather than curved. 相似文献
20.
Two anthiine fishes from the eastern tropical Atlantic are described as newHolanthias cyprinoides andAnthias helenensis. The former is distinguished from the other Atlantic species ofHolanthias in having the forked caudal fin with rounded lobes and from the Indo-Pacific species in having no elongated dorsal spines or soft rays. The latter is closely related toAnthias asperilinguis Günther (South America, Atlantic coast), but differs from it in having more pectoral fin rays and more gill rakers. The present investigation onAnthias suggests that AtlanticAnthias is a genus distinct fromPseudanthias of the Indo-Pacific. 相似文献