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41.
Fresh-water planarias and a marine planaria are relatively dissimilar in the 5S rRNA sequences. 总被引:3,自引:3,他引:0 下载免费PDF全文
The nucleotide sequences from fresh-water Dugesia japonica and marine Planocera reticulata have been determined. The similarity between these two species is only 69%. The Planocera sequence reveals nearly 80% similarity (72-81%) to the sequences of multicellular animals, while the Dugesia sequences are considerably different from them (66-73%). 相似文献
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K. Hori 《Entomologia Experimentalis et Applicata》1969,12(4):454-466
Some properties of salivary amylases of the adults of Adelphocoris suturalis Jakovlev and Dolycoris baccarum L. were studied in vitro and compared with those of several other heteropteran species. The reducing sugar produced by the action of the salivary amylase of D. baccarum increased in proportion to substrate concentration while the concentration was relatively low (below 0.67% in its final concentration). Its increase stopped at the concentrations from 0.67 to 2.0%, and then it increased again constantly but slowly. The optimum temperature for the action of the enzyme was found to be about 50° (in A. suturalis) and about 40° (in D. baccarum), and the optimal pH was 3.5–4.0 in A. suturalis and 6.0 in D. baccarum. The salivary amylase activity of D. baccarum was scarcely affected by NaCl, KNO3, and other compounds, while the salivary amylase of A. suturalis was strongly activated by NaCl and moderately by KNO3. It seems highly probable that taxonomically related heteropterous insects might well be grouped with respect to the degree of activation of the salivary amylase by Cl- or NO3-.
Contribution No. 28 from the Laboratory of Entomology, Obihiro Zootechnical University. 相似文献
Zusammenfassung Einige Eigenschaften der Speichelamylase von Adelphocoris suturalis und Dolycoris baccarum wurden im Reagenzglas studiert und mit denen anderer Heteropterenarten verglichen. Die durch die Wirkung der Speichelamylase von D. baccarum produzierten Stärkehydrolysate vermehrten sich im Verhältnis zur Substratkonzentration bei einer verhältnismäßig niedrigen Konzentration (weniger als 0,67% in der Schlußkonzentration). Diese Vermehrung wurde bei einer Konzentration von etwa 0,67 bis 2,0 unterbrochen, um sich dann wieder stetig, aber langsamer fortzusetzen. Die optimale Temperatur für die Wirkung des Fermentes lag für A. suturalis bei etwa 50° und für D. baccarum bei etwa 40°. Die optimale Wasserstoff-Ionenkonzentration betrug für A. suturalis pH 3,5–4,0 und für D. baccarum pH 6,0. Die Aktivität der Speichelamylase von D. baccarum wurde durch NaCl, KNO3 und andere Verbindungen kaum beeinflußt, während die Speichelamylase von A. suturalis durch NaCl stark und durch KNO3 schwach aktiviert wurde. Es wird vermutet, daß, hinsichtlich des Aktivierungsgrades der Speichelamylase durch Cl- und NO3-, systematisch nahe verwandte Heteropterenarten der gleichen Gruppe angehören.
Contribution No. 28 from the Laboratory of Entomology, Obihiro Zootechnical University. 相似文献
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The factors stimulating estrogen secretion in the preovulatory phase and an attempt to explain the mechanism of termination of estrogen secretion are discussed. Female Wistar rats, hypophysectomized at 1 p.m. in proestrus, were injected with rat pituitary extracts. Ovarian venous blood was collected and the estrogen activity of the plasma was measured. The estrogen secretion was minimized within 3 hours after hypophysectomy. The rat pituitary extract caused an 11-fold increase of estrogen concentration in the ovarian venous blood within 1 hour. Either LH or FSH alone was able to restore the estrogen secretion: LH took 1 hour to reach maximal response, FSH 2 hours. In the 1-hour test, the minimal effective dose for LH appeared to be less than .25 mcg per rat, for FSH, 2.5 mcg per rat. The total ability of the two preparations to produce estrogen appeared to be the same. 10 I.U. of prolactin slightly stimulated estrogen secretion, but 20 mU of ACTH was quite negative. These results demonstrate the pituitary gonadotropin dependency of estrogen secretion from the ovary having ripened follicles. It also showed that the ovary, after completion of ovulatory surge of LH, abolished its reactivity to the pituitary extract containing sufficient amount of substances in promoting estrogen secretion. Either LH or FSH was able to terminate estrogen secretion even at minute doses as small as 10 mcg. This shows that both FSH and LH provide a dual effect on ovarian estrogen secretion at the preovulatory stage, promotion and suppression. Promotion is an acute and direct action of hormones on steroidogenesis and suppression probably a delayed and indirect action of ovulation-inducing hormone, the release of which initiates the differentiation of estrogen-forming cells towards ovulation unfavorable to estrogen synthesis. 相似文献
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Cleavage of dengue virus NS1-NS2A requires an octapeptide sequence at the C terminus of NS1. 总被引:15,自引:12,他引:3 下载免费PDF全文
The length of amino acid sequence at the NS1-NS2A juncture of dengue virus that is required for specific cleavage effected by the cis-acting function of NS2A was identified by deletion analysis. Recombinant DNA sequences of NS1-NS2A, each containing a deletion in NS1 followed by a sequence of 3 to 20 amino acids at the C terminus of NS1 preceding the cleavage site, were constructed and expressed with vaccinia virus as a vector. The NS1 product of recombinant vaccinia virus-infected cells was immunoprecipitated and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The occurrence of cleavage between NS1 and NS2A was indicated by the appearance of shortened NS1. Failure to cleave this site yielded a large NS1-NS2A fusion protein. This analysis indicated that a minimum length of eight amino acids at the NS1 C terminus preceding the NS1-NS2A juncture is required for cleavage to take place. Comparison of this eight-amino-acid sequence of the NS1 C terminus of dengue type 4 virus with the analogous sequences of 12 other flaviviruses suggests that the consensus cleavage site sequence is as follows: (table; see text) 相似文献
50.
K Hori Y Yamamoto T Minetoki T Kurotsu M Kanda S Miura K Okamura J Furuyama Y Saito 《Journal of biochemistry》1989,106(4):639-645
The entire gene for gramicidin S synthetase 1 (GS 1) was cloned into the plasmid vector pUC18, and the nucleotide sequences of the GS 1 gene and its flanking region were determined. The full-length clone was 4,539 base pairs long and had an open reading frame of 3,294 nucleotides coding for 1,098 amino acids. The calculated molecular weight of 123,474 agreed with the apparent molecular weight of 120,000 found in SDS-PAGE of GS 1 from B. brevis. The nucleotide sequence of GS 1 gene was highly homologous to that of tyrocidine synthetase 1. The overall similarity between the deduced amino acid sequences of the two genes was 57.5%. The gene product of clone GS309 was easily purified to an essentially homogeneous state by ammonium sulfate fractionation followed by DEAE-Sepharose CL-6B, Ultrogel AcA-34, and second DEAE-Sepharose CL-6B column chromatography. The purified protein catalyzed the D-phenylalanine-dependent ATP-32PPi exchange reaction which is specific for GS 1 activity, and the specific activity of the purified product was nearly the same as the purified GS 1 from B. brevis. The product also showed a weak phenylalanine racemase activity. 相似文献