全文获取类型
收费全文 | 98篇 |
免费 | 10篇 |
专业分类
108篇 |
出版年
2022年 | 1篇 |
2019年 | 1篇 |
2015年 | 2篇 |
2014年 | 5篇 |
2013年 | 6篇 |
2012年 | 7篇 |
2011年 | 2篇 |
2010年 | 5篇 |
2009年 | 10篇 |
2008年 | 2篇 |
2007年 | 5篇 |
2006年 | 2篇 |
2005年 | 3篇 |
2004年 | 2篇 |
2003年 | 3篇 |
2002年 | 2篇 |
2001年 | 1篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 6篇 |
1997年 | 3篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1989年 | 4篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1974年 | 2篇 |
1972年 | 2篇 |
1948年 | 1篇 |
1946年 | 2篇 |
1943年 | 1篇 |
1942年 | 1篇 |
1941年 | 2篇 |
1934年 | 1篇 |
排序方式: 共有108条查询结果,搜索用时 15 毫秒
81.
Judit Dobránszki Katalin Magyar-Tábori Etelka Tombácz 《Plant biotechnology reports》2011,5(4):345-352
The rheological and diffusion properties of blends of agar/guar gum, agar/Phytagel and Phytagel/guar gum were analysed and
compared to those properties of agar or Phytagel applied alone at two different gelling concentrations. Moreover, their effects
on the shoot multiplication of the apple scion Galaxy and two black locust clones (SF63, SF82) were studied, and their cost
benefits over agar were calculated. Elastic hydrogel formation was demonstrated for each blend by rheological measurements,
but the gel strength depended on the types and concentrations of the applied gelling agents and blends. Guar gum was able
to speed the diffusion in the different blends, and diffusion was independent of gel strength. The rate of shoot multiplication
increased (to 8.9 shoots per explant) and the percent of hyperhydrated shoots decreased (to 12%) when the blend of agar/guar
gum was used for the shoot multiplication of apple. Similarly, the highest multiplication rates of black locust clones (between
3.9 and 4.1) were obtained on media solidified by blends containing guar gum. The best shoot performance with the lowest percent
of hyperhydrated shoots (11–12% in SF63 and 2–23% in SF82) was achieved using agar alone or the agar/guar gum blend. The shoot
multiplication was improved of both species and the production cost was reduced by 42% by using the agar/guar gum blend. 相似文献
82.
Quezada-Rivera JJ RE Soria-Guerra FS Pérez-Juárez L Martínez-González SE Valdés- Rodríguez NL Vasco-Méndez JF Morales-Domínguez 《Phyton》2019,88(1):25-35
The use of antimicrobial peptides (AMPs) synthesized
by bacteria (bacteriocins) is an alternative for combating multidrug
resistant bacterial strains and their production by recombinant route
is a viable option for their mass production. The bacteriocin E-760
isolated from the genus Enterococcus sp. has been shown to possess
inhibitory activity against Gram-negative and Gram-positive
bacteria. In this study, the expression of a chimeric protein coding
for E-760 in the nucleus of C. reinhardtii was evaluated, as well as,
its antibacterial activity. The synthetic gene E-760S was inserted
into the genome of C. reinhardtii using Agrobacterium tumefaciens.
A transgenic line was identified in TAP medium with hygromycin
and also by PCR. The increment in the culture medium temperature
of the transgenic strain at 35 °C for 10 minutes, increased the
production level of the recombinant protein from 0.14 (Noninduced
culture, NIC) to 0.36% (Induced culture, IC) of total soluble
proteins (TSP); this was quantified by an ELISA assay. Recombinant
E-760 possesses activity against Staphylococcus aureus in 0.34 U
log, Streptococcus agalactiae in 0.48 U log, Enterococcus faecium in
0.36 U log, Pseudomonas aeruginosa in 2 U log and for Klebsiella
pneumoniae, the activity was 0.07 U log. These results demonstrate
that the nucleus transformation of C. reinhardtii can function as
a stable expression platform for the production of the synthetic
gene E-760 and it can potentially be used as an antibacterial agent. 相似文献
83.
84.
85.
Glatz K Danka J Tombácz Z Bányai T Szilágyi A Kucsera I 《Acta microbiologica et immunologica Hungarica》2012,59(2):225-238
In a small village of Hungary, a human trichinellosis outbreak (affecting eight people) occurred in January-February, 2009. In the outbreak investigation (i) Trichinella spiralis larvae were detected in meat products derived from the pigs slaughtered in the backyard of one of the patients (a foxhunter) in December 2008, and in a brown rat captured in the same backyard; (ii) sera of 24 pigs held in 11 yards of the village and that of some dogs of the foxhunter were found Trichinella-positive; (iii) sera of five villagers who could not be infected in the particular outbreak were also found reactive in Trichinella-specific laboratory tests. The followings helped the rise of an outbreak: the geographical position and the presence of empty houses favoured the multiplication of rats; there was no extermination of rats in the previous years; there was no meat inspection; raw meat and improperly processed meat products were tasted at the pig-slaughter; villagers gave tastes to each other. People were informed on the symptoms, the way of transmission, and the possibilities of prevention of trichinellosis by experts. With the help of local authorities, all the properties including the grounds with empty houses were involved in the extermination of rodents. 相似文献
86.
87.
88.
89.
Background
AP65 is a prominent adhesin of Trichomonas vaginalis that mediates binding of parasites to host vaginal epithelial cells (VECs). AP65 with no secretion signal sequence, membrane targeting peptide, and anchoring motif was recently found to be secreted. 相似文献90.
Characterizing microbial diversity in production water from an Alaskan mesothermic petroleum reservoir with two independent molecular methods 总被引:2,自引:0,他引:2
Pham VD Hnatow LL Zhang S Fallon RD Jackson SC Tomb JF DeLong EF Keeler SJ 《Environmental microbiology》2009,11(1):176-187
The phylogenetic diversity of Bacteria and Archaea within a biodegraded, mesothermic petroleum reservoir in the Schrader Bluff Formation of Alaska was examined by two culture-independent methods based on fosmid and small-subunit rRNA gene PCR clone libraries. Despite the exclusion of certain groups by each method, there was overall no significant qualitative difference in the diversity of phylotypes recovered by the two methods. The resident Bacteria belonged to at least 14 phylum-level lineages, including the polyphyletic Firmicutes , which accounted for 36.2% of all small-subunit rRNA gene-containing (SSU+ ) fosmid clones identified. Members of uncultured divisions were also numerous and made up 35.2% of the SSU+ fosmid clones. Clones from domain Archaea accounted for about half of all SSU+ fosmids, suggesting that their cell numbers were comparable to those of the Bacteria in this microbial community. In contrast to the Bacteria , however, nearly all archaeal clones recovered by both methods were related to methanogens, especially acetoclastic methanogens, while the plurality of bacterial fosmid clones was affiliated with Synergistes -like acetogenic Firmicutes that possibly degrade longer-chain carboxylic acid components in the crude oil to acetate. These data suggest that acetate may be a key intermediary metabolite in this subsurface anaerobic food chain, which leads to methane production as the primary terminal electron sink. 相似文献