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201.
Best T Li D Skol AD Kirchhoff T Jackson SA Yasui Y Bhatia S Strong LC Domchek SM Nathanson KL Olopade OI Huang RS Mack TM Conti DV Offit K Cozen W Robison LL Onel K 《Nature medicine》2011,17(8):941-943
Survivors of pediatric Hodgkin's lymphoma are at risk for radiation therapy-induced second malignant neoplasms (SMNs). We identified two variants at chromosome 6q21 associated with SMNs in survivors of Hodgkin's lymphoma treated with radiation therapy as children but not as adults. The variants comprise a risk locus associated with decreased basal expression of PRDM1 (encoding PR domain containing 1, with ZNF domain) and impaired induction of the PRDM1 protein after radiation exposure. These data suggest a new gene-exposure interaction that may implicate PRDM1 in the etiology of radiation therapy-induced SMNs. 相似文献
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Slaastad H Wu W Goullart L Kanderova V Tjønnfjord G Stuchly J Kalina T Holm A Lund-Johansen F 《Proteomics》2011,11(23):4578-4582
Antibody array analysis of complex samples requires capture reagents with exceptional specificity. The frequency of antibodies with label-based detection may be as low as 5%. Here, however, we show that as many as 25% of commercially available antibodies are useful when biotinylated cellular proteins are fractionated by size exclusion chromatography (SEC) first. A microsphere multiplex with 1725 antibodies to cellular proteins was added to 24 SEC fractions, labelled with streptavidin and analyzed by flow cytometry (microsphere-based affinity proteomics, MAP) The SEC-MAP approach resolved different targets captured by each antibody as reactivity peaks across the separation range of the SEC column (10-670kDa). Complex reactivity profiles demonstrated that most antibodies bound more than one target. However, specific binding was readily detected as reactivity peaks common for different antibodies to the same protein. We optimized sample preparation and found that amine-reactive biotin rarely inhibited antibody binding when the biotin to lysine ratio was kept below 1:1 during labelling. Moreover, several epitopes that were inaccessible to antibodies in native proteins were unmasked after heat denaturation with 0.1% of SDS. The SEC-MAP format should allow researchers to build multiplexed assays with antibodies purchased for use in e.g. Western blotting. 相似文献
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The theory of natural selection has been vital in unifying the biological sciences and their research with a single testable
metatheory. Despite a plethora of research supporting natural selection, teaching the theory of evolution remains controversial
in high schools and higher education (Wilson et al. 2009; Scott 1997). In this article, we sample the attitudes toward evolution of 170 faculty and graduate and undergraduate students in family
studies and human development programs from across the United States to determine whether resistances toward evolution remain
and to describe the correlates of these resistances. Results reveal that an individual’s prosocial meliorist attitudes, religious
ideation, and his or her reported interest in and knowledge of evolution all uniquely contribute to whether they report evolutionary
theory as being applicable to their area of research interests. We discuss the relevance of including evolutionary theory
within family studies and human development research programs and make suggestions for how to implement an evolutionary studies
program (Wilson et al. 2009). 相似文献
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Jacobsson JA Almén MS Benedict C Hedberg LA Michaëlsson K Brooks S Kullberg J Axelsson T Johansson L Ahlström H Fredriksson R Lind L Schiöth HB 《PloS one》2011,6(5):e20158