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Ono E Hatayama M Isono Y Sato T Watanabe R Yonekura-Sakakibara K Fukuchi-Mizutani M Tanaka Y Kusumi T Nishino T Nakayama T 《The Plant journal : for cell and molecular biology》2006,45(2):133-143
Aureusidin synthase, a polyphenol oxidase (PPO), specifically catalyzes the oxidative formation of aurones from chalcones, which are plant flavonoids, and is responsible for the yellow coloration of snapdragon (Antirrhinum majus) flowers. All known PPOs have been found to be localized in plastids, whereas flavonoid biosynthesis is thought to take place in the cytoplasm [or on the cytoplasmic surface of the endoplasmic reticulum (ER)]. However, the primary structural characteristics of aureusidin synthase and some of its molecular properties argue against localization of the enzyme in plastids and the cytoplasm. In this study, the subcellular localization of the enzyme in petal cells of the yellow snapdragon was investigated. Sucrose-density gradient and differential centrifugation analyses suggested that the enzyme (the 39-kDa mature form) is not located in plastids or on the ER. Transient assays using a green fluorescent protein (GFP) chimera fused with the putative propeptide of the PPO precursor suggested that the enzyme was localized within the vacuole lumen. We also found that the necessary information for vacuolar targeting of the PPO was encoded within the 53-residue N-terminal sequence (NTPP), but not in the C-terminal sequence of the precursor. NTPP-mediated ER-to-Golgi trafficking to vacuoles was confirmed by means of the co-expression of an NTPP-GFP chimera with a dominant negative mutant of the Arabidopsis GTPase Sar1 or with a monomeric red fluorescent protein (mRFP)-fused Golgi marker (an H+-translocating inorganic pyrophosphatase of Arabidopsis). We identified a sequence-specific vacuolar sorting determinant in the NTPP of the precursor. We have demonstrated the biosynthesis of a flavonoid skeleton in vacuoles. The findings of this metabolic compartmentation may provide a strategy for overcoming the biochemical instability of the precursor chalcones in the cytoplasm, thus leading to the efficient accumulation of aurones in the flower. 相似文献
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Role of roots in differences in seed cadmium concentration among soybean cultivars—proof by grafting experiment 总被引:2,自引:0,他引:2
Soybean cultivars show significant differences in cadmium (Cd) concentrations in seeds, due primarily to genetics, not environmental
factors. We previously suggested that low-Cd cultivars accumulate Cd in their roots and thus prevent its translocation to
the rest of the plant. Through grafting experiments, we drew the following conclusions about Cd absorption and translocation:
(1) The amount of Cd accumulated in shoots is determined by the Cd accumulation capacity of roots: cultivars with a small
capacity to accumulate Cd in roots translocate more Cd and accumulate it in shoots; (2) The Cd concentration in shoots is
determined by the Cd accumulation capacity of roots and the shoot productive ability of the scion cultivar; (3) The Cd tolerance
of shoots differs among cultivars. Enrei, with a high-Cd accumulation capacity of roots, had a low Cd tolerance of shoots
compared with Suzuyutaka and Hatayutaka, with a low Cd accumulation capacity of roots; (4) Cultivars differ in their distribution
of Cd to seed; (5) These results show that seed Cd concentration is influenced by the differences among cultivars in ease
of translocation of Cd to seed and in Cd accumulation capacity of roots. 相似文献
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An endoplasmic reticulum protein, p180, is highly expressed in human cytomegalovirus-permissive cells and interacts with the tegument protein encoded by UL48 下载免费PDF全文
Ogawa-Goto K Irie S Omori A Miura Y Katano H Hasegawa H Kurata T Sata T Arao Y 《Journal of virology》2002,76(5):2350-2362
We have used a virus overlay assay to detect cellular proteins associated with human cytomegalovirus (HCMV) particles. The radiolabeled HCMV particles specifically bound to two host proteins with molecular sizes of 150 and 180 kDa. By a micro-amino-acid sequencing technique, the 180-kDa protein was identified as a human homologue of the ES130/p180 ribosome receptor (p180), which is an integral endoplasmic reticulum (ER) membrane protein possessing a very unique tandem repeat domain at its N-terminal region. The virus overlay assay using truncated p180 polypeptides revealed that HCMV binding to human p180 occurred through the N-terminal region. In HCMV-permissive cells the high level of expression of the human p180 protein was clearly observed regardless of cell type. Furthermore, we showed that p180 binds to the UL48 gene product, which is one of the predominant tegument proteins of HCMV and which is considered to be tightly associated with the capsid. The interaction between the two proteins was assumed to be specific and was observed both in vitro and in vivo. During the late phase of infection, the unique relocation of human p180 was observed, that is, to the juxtanuclear region, which appeared to be in the vicinity of the area where naked virions were frequently observed in an electron-microscopic study. Thus our data suggest that p180 interacts with the HCMV tegument, at least through pUL48, during the HCMV replication process. We discuss the possible role of the interaction between p180 and pUL48 in the intracellular transport of HCMV virions. 相似文献