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排序方式: 共有125条查询结果,搜索用时 15 毫秒
111.
I Ono I Ishiwata T Nakaguchi K Konoe N Tokita M Soma C Ishiwata H Ishikawa 《Human cell》1989,2(1):74-79
The heterotransplantability of HOTHC line (human anaplastic thyroid carcinoma) into the subcutis of BALB/c nude mice and the biological properties of grafts were discussed. (1) The HOTHC line showed high transplantability, and 1 x 10(4) cells produced anaplastic carcinoma (giant cell type) containing the colloid-like substance. (2) The grafted tumors grew rapidly and the mice were dead within 2 months after transplantation. (3) The number of leukocytes (neutrophils) of mouse peripheral blood increased as the tumor size increased, and the leukocyte count returned to a normal value after removal of the tumor. (4) The conditioned media of HOTHC line formed colonies of granulocytes (neutrophils) on soft agar. These phenomena revealed that HOTHC is a granulocyte-colony stimulating factor-producing line. (5) The conditioned media of HOTHC line showed promoting-effect on neovascularization on the chorioallantoic membrane. 相似文献
112.
Frogs have highly conserved hand and foot morphology, possessing four fingers and five toes. As an exception, two Japanese ranid frog species, the Otton frog Babina subaspera and the dagger frog Babina holsti, possess a unique thumb-like structure (the pseudothumb) in the forelimb, giving an appearance of a total of five fingers on the hand. To obtain insights into the developmental mechanisms that generate this novel character, we investigated the hand morphogenesis of the Otton frog. The unique morphological pattern of the pseudothumb was already established in juveniles. Surprisingly, the bud-like structure, which is similar to the area of inductive activity (e.g. feather buds in birds and the carapacial ridge in turtles), was detected over the site where the future prepollex develops in larvae. By contrast, this bud-like structure was not found in larvae of other ranid species. We discuss possible scenarios that would favour the evolution of this very unusual trait in frogs. 相似文献
113.
Takuya Shuo Sachiko Aono Fumiko Matsui Yoshihito Tokita Hiroshi Maeda Katsuhiko Shimada Atsuhiko Oohira 《Glycoconjugate journal》2003,20(4):267-278
Neuroglycan C (NGC), a brain-specific transmembrane proteoglycan, is thought to bear not only chondroitin sulfate but also
N- and O-linked oligosaccharides on its core protein. In this study, we isolated and purified NGC from rat brains at various
developmental stages by immunoaffinity column chromatography or by immunoprecipitation, and examined the structural characters
of its carbohydrate moiety. The chondroitin sulfate disaccharide composition of NGC at postnatal day 10 was significantly
different from those of two secreted chondroitin sulfate proteoglycans, neurocan and phosphacan, purified from the brain at
the same developmental stage; higher levels of 4-sulfate unit and E unit, a disulfated disaccharide unit, and a lower level
of 6-sulfate unit. The levels of both 6-sulfate and E units decreased with a compensatory increase of 4-sulfate unit with
postnatal development of the brain. Lectin-blot analysis of the NGC core glycoprotein prepared by chondroitinase digestion
confirmed that NGC actually bore both N- and O-linked carbohydrates, and also revealed that lectin-species reactive with NGC
did not always recognize other brain-specific proteoglycans, neurocan and phosphacan, and vice versa, even though they were
isolated from the brain at the same stage. The reactivity of NGC with lectins and with the HNK-1 antibody markedly changed
as the brain matured. These findings indicate that the structure of the carbohydrate moiety of NGC is developmentally regulated,
and differs from those of neurocan and phosphacan. The developmentally-regulated structural change of the carbohydrates on
NGC may be partly implicated in the modulation of neuronal cell recognition during brain development. Published in 2004.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
114.
Unno A Takada I Takezawa S Oishi H Baba A Shimizu T Tokita A Yanagisawa J Kato S 《Biochemical and biophysical research communications》2005,327(3):933-938
TBP-free TAF II-containing-type HAT complex subclasses, which contain hGCN5 HAT and TRRAP, appear to act as common coactivator complexes for nuclear receptors. However, their physiological significance with respect to each nuclear receptor remains to be established. To address this issue, we used hepatic cell lines (HepG2) with reduced endogenous TRRAP expression through antisense RNA expression or with overexpressed TRRAP or other major coactivators. The ligand-induced transactivation function of liver X receptor alpha (LXRalpha) and farnesoid X receptor/bile acid receptor reflected TRRAP expression levels, while that of PPARgamma did not. A GST pull-down assay indicated that TRRAP contains two potential LXRalpha-interacting domains in the C-terminal and central domains. Expression of antisense TRRAP RNA in HepG2 cells abolished the ligand-induced expression of LXRalpha target genes. These results suggested that TRRAP plays an important role as a coactivator, presumably part of a complex, in lipid metabolism through regulation of the LXRalpha-mediated gene cascade in hepatic cells. 相似文献
115.
Reptilian skull morphology is highly diverse and broadly categorized into three categories based on the number and position of the temporal fenestrations: anapsid, synapsid, and diapsid. According to recent phylogenetic analysis, temporal fenestrations evolved twice independently in amniotes, once in Synapsida and once in Diapsida. Although functional aspects underlying the evolution of tetrapod temporal fenestrations have been well investigated, few studies have investigated the developmental mechanisms responsible for differences in the pattern of temporal skull region. To determine what these mechanisms might be, we first examined how the five temporal bones develop by comparing embryonic cranial osteogenesis between representative extant reptilian species. The pattern of temporal skull region may depend on differences in temporal bone growth rate and growth direction during ontogeny. Next, we compared the histogenesis patterns and the expression of two key osteogenic genes, Runx2 and Msx2, in the temporal region of the representative reptilian embryos. Our comparative analyses suggest that the embryonic histological condition of the domain where temporal fenestrations would form predicts temporal skull morphology in adults and regulatory modifications of Runx2 and Msx2 expression in osteogenic mesenchymal precursor cells are likely involved in generating morphological diversity in the temporal skull region of reptiles. 相似文献
116.
Tsujimura S Fukuda J Shirai O Kano K Sakai H Tokita Y Hatazawa T 《Biosensors & bioelectronics》2012,34(1):244-248
The mediated electro-enzymatic electrolysis systems based on the tricarboxylic acid (TCA) cycle reaction were examined on a micro-bulk electrolytic system. A series of the enzyme-catalyzed reactions in the TCA cycle was coupled with electrode reaction. Electrochemical oxidation of NADH was catalyzed by diaphorase with an aid of a redox mediator with a formal potential of -0.15 V vs. Ag|AgCl. The mediator was also able to shuttle electrons between succinate dehydrogenase and electrode. The charge during the electrolysis increased on each addition of dehydrogenase reaction in a cascade of the TCA cycle. However, the electrolysis efficiencies were close to or less than 90% because of the product inhibition. Lactate oxidation to acetyl-CoA catalyzed by two NAD-dependent dehydrogenases was coupled with the bioelectrochemical TCA cycle reaction to achieve the 12-electron oxidation of lactate to CO(2). The charge passed in the bioelectrocatalytic oxidation of 5 nmol of lactate was 4 mC, which corresponds to 70% of the electrolysis efficiency. 相似文献
117.
Yuko Mitobe Sayaka Ito Takashi Mizutani Tsuyoshi Nagase Nagaaki Sato Shigeru Tokita 《Bioorganic & medicinal chemistry letters》2009,19(15):4075-4078
Radioligands are powerful tools for examining the pharmacological profiles of chemical leads and thus facilitate drug discovery. In this study, we identified and characterized 3-([1,1,1-3H]methyl)-2-(4-{[3-(1-pyrrolidinyl)propyl]oxy} phenyl)-4(3H)-quinazolinone ([3H]1) as a potent and selective radioligand for histamine H3 receptors. Radioligand [3H]1 exhibited appreciable specific signal in brain slices prepared from wild-type mice but not from histamine H3 receptor-deficient mice, demonstrating the specificity and utility of [3H]1 as a selective histamine H3 receptor radioligand for ex-vivo receptor occupancy assays. 相似文献
118.
Takahide Sasaki Toshiyuki Takahashi Tsuyoshi Nagase Takashi Mizutani Sayaka Ito Yuko Mitobe Yasuhisa Miyamoto Maki Kanesaka Ryo Yoshimoto Takeshi Tanaka Norihiro Takenaga Shigeru Tokita Nagaaki Sato 《Bioorganic & medicinal chemistry letters》2009,19(15):4232-4236
A series of novel dihydrobenzoxathiin derivatives was synthesized and evaluated as potent human histamine H3 receptor inverse agonists. After systematic modification of lead 1a, the potent and selective histamine H3 inverse agonist 1-(3-{4-[(2S,3S)-8-methoxy-3-methyl-4,4-dioxido-2,3-dihydro-1,4-benzoxathiin-2-yl]phenoxy}propyl)pyrrolidine (5k) was identified. Compound 5k showed good pharmacokinetic profiles and brain penetrability in laboratory animals. After 3 mg/kg oral administration of 5k, significant elevation of brain histamine levels was observed in rats where the brain H3 receptor was fully occupied. 相似文献
119.
Swe1Wee1-Dependent Tyrosine Phosphorylation of Hsp90 Regulates Distinct Facets of Chaperone Function
Mehdi Mollapour Shinji Tsutsumi Alison C. Donnelly Kristin Beebe Mari J. Tokita Min-Jung Lee Sunmin Lee Giulia Morra Dimitra Bourboulia Bradley T. Scroggins Giorgio Colombo Brian S. Blagg Barry Panaretou William G. Stetler-Stevenson Jane B. Trepel Peter W. Piper Chrisostomos Prodromou Laurence H. Pearl Len Neckers 《Molecular cell》2010,37(3):333-343
120.