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141.
142.
Hiroyuki Matsuda Tokio Wada Yasuhiro Takeuchi Yoshiharu Matsumiya 《Population Ecology》1992,34(2):309-319
There are two factors affecting long-term fluctuation of planktotrophic pelagic fish: environmental fluctuation and interspecific competition. Long-term catch data of planktotrophic pelagic fishes in Japan suggest that the chub mackerel (species B) was replaced by the sardine (A), A was replaced by the anchovy, Pacific saury and horse mackerel (Group C), and species in group C were replaced by species B. If species A defeats B, B defeats C, and C defeats A in interspecific competitive ability, then the abundance of these three groups fluctuate forever and dominate in the same order. We call this cyclic advantage hypothesis for species replacement. In this model, environmental fluctuation affects the species replacement as a trigger. Environmental fluctuation does not determine the next dominant species but greatly affects when the next replacement occurs. 相似文献
143.
144.
Yoji Nakamura Naobumi Sasaki Masahiro Kobayashi Nobuhiko Ojima Motoshige Yasuike Yuya Shigenobu Masataka Satomi Yoshiya Fukuma Koji Shiwaku Atsumi Tsujimoto Takanori Kobayashi Ichiro Nakayama Fuminari Ito Kazuhiro Nakajima Motohiko Sano Tokio Wada Satoru Kuhara Kiyoshi Inouye Takashi Gojobori Kazuho Ikeo 《PloS one》2013,8(3)
Nori, a marine red alga, is one of the most profitable mariculture crops in the world. However, the biological properties of this macroalga are poorly understood at the molecular level. In this study, we determined the draft genome sequence of susabi-nori (Pyropia yezoensis) using next-generation sequencing platforms. For sequencing, thalli of P. yezoensis were washed to remove bacteria attached on the cell surface and enzymatically prepared as purified protoplasts. The assembled contig size of the P. yezoensis nuclear genome was approximately 43 megabases (Mb), which is an order of magnitude smaller than the previously estimated genome size. A total of 10,327 gene models were predicted and about 60% of the genes validated lack introns and the other genes have shorter introns compared to large-genome algae, which is consistent with the compact size of the P. yezoensis genome. A sequence homology search showed that 3,611 genes (35%) are functionally unknown and only 2,069 gene groups are in common with those of the unicellular red alga, Cyanidioschyzon merolae. As color trait determinants of red algae, light-harvesting genes involved in the phycobilisome were predicted from the P. yezoensis nuclear genome. In particular, we found a second homolog of phycobilisome-degradation gene, which is usually chloroplast-encoded, possibly providing a novel target for color fading of susabi-nori in aquaculture. These findings shed light on unexplained features of macroalgal genes and genomes, and suggest that the genome of P. yezoensis is a promising model genome of marine red algae. 相似文献
145.
We confirmed that Rhyacodrilus spp of Yasuda and Okino (1987) (Tubificidae, Oligochaeta) migrated entirely downward below 15 cm into the sediment from June to September m Lake Suwa, a shallow eutrophic lake in Japan In October, Rhyacodrilus spp began to return to the upper layer and immediately attained sexual maturity They tended to show reduced body weight and respiratory activity during summer The results indicate that an oligochaete of Rhyacodrilus spp estivated in the deeper layer during summer The estivation ostensibly occurred at 15°C and the species died off at 20°C, so it is evident that they must migrate from the surface to the deeper layer of sediment during summer in order to escape high temperature Furthermore, physiological experiment suggested that this behavior is accelerated by low oxygen concentration with high temperature 相似文献
146.
147.
Toshiharu Nagatsu Tokio Yamaguchi Takeshi Kato Takashi Sugimoto Sadao Matsuura Miki Akino Shoichiro Tsushima Nobuhiko Nakazawa Hiroshi Ogawa 《Analytical biochemistry》1981,110(1):182-189
Specific antibodies against l-erythro-biopterin have been prepared in rabbits using the conjugates to bovine serum albumin. The antiserum against l-erythro-biopterin distinguished among l-erythro-tetrahydro- or 7,8-dihydro-biopterin, the other three stereoisomers of biopterin, d-erythro-neopterin, folic acid, and other synthetic pteridines. Using the specific antiserum against l-erythro-biopterin, a radioimmunoassay has been developed to measure the biopterin concentrations in urine, serum, cerebrospinal fluid, and tissues. The conjugate of l-erythro-biopterin with tyramine, 4-hydroxy-2-[2-(4-hydroxyphenyl)ethylamino]-6-(l-erythro-1,2-dihydroxypropyl)pteridine (BP-TYRA), was synthesized and labeled with 125I as the labeled ligand for the radioimmunoassay. BP-125I-TYRA had similar binding affinity as the natural l-erythro-biopterin and was thus permitted to establish a highly sensitive radioimmunoassay for biopterin. The limit of sensitivity of the radioimmunoassay with BP-125I-TYRA as labeled ligand was 0.5 pmol. The total concentration of biopterins, i.e., biopterin, 7,8-dihydro-, quinonoid dihydro and tetrahydrobiopterins, in the biological samples was obtained by iodine oxidation under acidic conditions prior to the radioimmunoassay, whereas iodine oxidation under alkaline conditions gave the concentration only of the former two. Biopterin in urine could be measured directly using 1 μl of urine, but a pretreatment with a small Dowex 50-H+ column was required for serum, cerebrospinal fluid, and brain tissues. 相似文献
148.
Mary?Jeanie?Telebanco-Yanoria Yohei?Koide Yoshimichi?Fukuta Tokio?Imbe Hiroshi?Tsunematsu Hiroshi?Kato Leodegario?A.?Ebron Thi?Minh?Nguyet?Nguyen Nobuya?KobayashiEmail author 《Molecular breeding : new strategies in plant improvement》2011,27(3):357-373
Twenty-seven near-isogenic lines (NILs) with the genetic background of a blast-susceptible variety, CO 39, were developed
by repeated backcrossing as a first set of a large number of differential varieties (DVs) with Indica-type genetic background.
The NILs included 14 resistance genes—Pish, Pib, Piz-5, Piz-t, Pi5(t), Pik-s, Pik, Pik-h, Pik-m, Pik-p, Pi1, Pi7(t), Pita, and Pita-2—derived from 26 donor varieties. The reaction patterns of NILs against 20 standard isolates from the Philippines were similar
to those of blast monogenic lines with the same resistance gene, except for those against two isolates that are avirulent
to Pia in the genetic background of CO 39. A genome-wide DNA marker survey revealed that chromosome segments were introgressed in
the regions where each resistance gene was previously mapped and most of the other chromosome regions in each NIL were CO
39 type. Segregation analysis of resistance and co-segregation analysis between resistance and DNA markers using F3 populations
derived from the crosses between each NIL and the recurrent parent, CO 39, revealed a single-gene control of resistance and
association between resistance and target introgressed segments. The morphological characters of each NIL were almost the
same as those of the recurrent parent except for some lines, suggesting that these NILs can be used even under tropical conditions
where Japonica-type DVs are not suitable for cropping. Thus, these NILs are useful not only as genetic tools for blast resistance
study but also as sources of genes for breeding of Indica-type rice varieties. 相似文献
149.
Privatdozent Dr. Shigeo Okinaka Dr. Tokio Sakurai Dr. Hirobumi Ôsuzu 《Cell and tissue research》1938,28(1):120-132
Zusammenfassung Die kleinen Zellen im Accessoriuskern und Hypoglossuskern, die in Form und Bau der Neben- und Mittelzelle Waldeyers im Rückenmark sehr nahe stehen, zeigen deutliche retrograde Veränderung nach der Durchtrennung des Accessorius bzw. Hypoglossus. Wir möchten dem-nach diese Zellen als extrapyramidalen Kern und die kleine markhaltige Faser im N. accessorius und hypoglossus als extrapyramidale Faser betrachten. 相似文献
150.
Tokio Domoto Makoto Teramoto Keiichiro Tanigawa Katsuhiro Tamura Yukihiko Yasui 《Cell and tissue research》1995,281(2):215-221
The origin of nitric oxide synthase-containing nerve fibers in rat celiac-superior mesenteric ganglion was examined using retrograde tracing techniques combined with the immunofluorescence method. Fluoro-Gold was injected into the celiac-superior mesenteric ganglion. Neuronal cell bodies retrogradely labeled with Fluoro-Gold in the thoracic spinal cord, the dorsal root ganglia at the thoracic level, the nodose ganglion, and the intestine from the duodenum to the proximal colon were examined for nitric oxide synthase immunoreactivity. About 60% of sympathetic preganglionic neurons in the intermediolateral nucleus projecting to the celiac-superior mesenteric ganglion were immunoreactive for nitric oxide synthase, as were approximately 27% of nodose ganglion neurons and about 65% of dorsal root ganglion neurons projecting to the cceliac-superior mesenteric ganglion. Neurons projecting to the celiac-superior mesenteric ganglion were found in the myenteric plexus of the small and large intestine. In the proximal colon, about 23% of such neurons were immunoreactive for nitric oxide synthase. However, in the small intestine, no immunoreactivity was found in these neurons. 相似文献