全文获取类型
收费全文 | 4030篇 |
免费 | 373篇 |
国内免费 | 3篇 |
出版年
2023年 | 27篇 |
2022年 | 58篇 |
2021年 | 131篇 |
2020年 | 79篇 |
2019年 | 92篇 |
2018年 | 115篇 |
2017年 | 106篇 |
2016年 | 170篇 |
2015年 | 309篇 |
2014年 | 275篇 |
2013年 | 270篇 |
2012年 | 444篇 |
2011年 | 359篇 |
2010年 | 230篇 |
2009年 | 186篇 |
2008年 | 267篇 |
2007年 | 240篇 |
2006年 | 196篇 |
2005年 | 189篇 |
2004年 | 132篇 |
2003年 | 129篇 |
2002年 | 107篇 |
2001年 | 17篇 |
2000年 | 18篇 |
1999年 | 12篇 |
1998年 | 27篇 |
1997年 | 9篇 |
1996年 | 8篇 |
1995年 | 12篇 |
1994年 | 10篇 |
1993年 | 12篇 |
1992年 | 16篇 |
1991年 | 13篇 |
1990年 | 9篇 |
1988年 | 12篇 |
1987年 | 5篇 |
1985年 | 5篇 |
1984年 | 13篇 |
1983年 | 7篇 |
1982年 | 6篇 |
1981年 | 9篇 |
1980年 | 4篇 |
1979年 | 8篇 |
1978年 | 4篇 |
1976年 | 4篇 |
1975年 | 8篇 |
1974年 | 4篇 |
1972年 | 4篇 |
1969年 | 4篇 |
1968年 | 4篇 |
排序方式: 共有4406条查询结果,搜索用时 15 毫秒
951.
Heiko Tobias Schumacher Evgenia Vamvaka Kriton Kalantidis 《The Plant journal : for cell and molecular biology》2016,88(5):839-853
Proteins belonging to the enhancer of RNA interference‐1 subfamily of 3′–5′ exoribonucleases participate in divergent RNA pathways. They degrade small interfering RNAs (siRNAs), thus suppressing RNA interference, and are involved in the maturation of ribosomal RNAs and the degradation of histone messenger RNAs (mRNAs). Here, we report evidence for the role of the plant homologue of these proteins, which we termed ENHANCED RNA INTERFERENCE‐1‐LIKE‐1 (ERIL1), in chloroplast function. In vitro assays with AtERIL1 proved that the conserved 3′–5′ exonuclease activity is shared among all homologues studied. Confocal microscopy revealed that ERL1, a nucleus‐encoded protein, is targeted to the chloroplast. To gain insight into its role in plants, we used Nicotiana benthamiana and Arabidopsis thaliana plants that constitutively overexpress or suppress ERIL1. In the mutant lines of both species we observed malfunctions in photosynthetic ability. Molecular analysis showed that ERIL1 participates in the processing of chloroplastic ribosomal RNAs (rRNAs). Lastly, our results suggest that the missexpression of ERIL1 may have an indirect effect on the microRNA (miRNA) pathway. Altogether our data point to an additional piece of the puzzle in the complex RNA metabolism of chloroplasts. 相似文献
952.
953.
954.
Life span extension by targeting a link between metabolism and histone acetylation in Drosophila 下载免费PDF全文
955.
956.
957.
Tobias Scheer 《Morphology》2016,26(3-4):341-378
The paper argues that morpho-syntactic computation and phonological melody (i.e. segmental primes occurring below the skeleton) are entirely incommunicado, in both directions. That is, there is no morpho-syntactic operation, say, movement, which goes into effect only if the moved item begins with a labial.This claim is run against the specific record of phonologically conditioned allomorphy (PCA). The prediction is that only non-melody, i.e. items located at and above the skeleton, may condition allomorphy: stress, tone, size, syllable structure, rhythm and the like. On the backdrop of Paster’s (2006) and Nevins’ (2011) cross-linguistic record of PCA, challenging cases are identified: those where the trigger is melodic without there being a plausible phonological pathway from the illegal to the legal alternant. It is shown that there is a purely phonological analysis of this pattern: the arbitrary relation between the illegal and the legal alternant is encoded in the lexicon, but in one and the same lexical entry (instead of two) where the illegal item is associated to a constituent and the rescue segment floats. When the regular (associated) segment is illegal in a given phonological context, it delinks and the floating substitute attaches to the position vacated.As a consequence, a plausible case can be made to the end that morphological computation never takes into account melodic information when selecting allomorphs: all candidate patterns have a purely phonological analysis based on one single underlier. This result is evaluated in the context of the debate regarding modularity: non-modular approaches that grant exhaustive simultaneous access to morphological and phonological information overgenerate, while the modularity-restricted architecture makes the correct prediction: the access of morphological computation to phonological information is selective (excluding melody). Finally, the multiple inputs analysis (Mascaró 2007) is shown to face the same problem, albeit only for a subset of PCA patterns, the ones that are non-optimizing. 相似文献
958.
Joshua Tobias Astrid Von Mentzer Patricia Loayza Frykberg Martin Aslett Andrew J. Page ?sa Sj?ling Ann-Mari Svennerholm 《PloS one》2016,11(4)
Enterotoxigenic Escherichia coli (ETEC), one of the most common reasons of diarrhea among infants and children in developing countries, causes disease by expression of either or both of the enterotoxins heat-labile (LT) and heat-stable (ST; divided into human-type [STh] and porcine-type [STp] variants), and colonization factors (CFs) among which CS6 is one of the most prevalent ETEC CFs. In this study we show that ETEC isolates expressing CS6+STh have higher copy numbers of the cssABCD operon encoding CS6 than those expressing CS6+STp. Long term cultivation of up to ten over-night passages of ETEC isolates harboring CS6+STh (n = 10) or CS6+STp (n = 15) showed instability of phenotypic expression of CS6 in a majority of the CS6+STp isolates, whereas most of the CS6+STh isolates retained CS6 expression. The observed instability was a correlated with loss of genes cssA and cssD as examined by PCR. Mobilization of the CS6 plasmid from an unstable CS6+STp isolate into a laboratory E. coli strain resulted in loss of the plasmid after a single over-night passage whereas the plasmid from an CS6+STh strain was retained in the laboratory strain during 10 passages. A sequence comparison between the CS6 plasmids from a stable and an unstable ETEC isolate revealed that genes necessary for plasmid stabilization, for example pemI, pemK, stbA, stbB and parM, were not present in the unstable ETEC isolate. Our results indicate that stable retention of CS6 may in part be affected by the stability of the plasmid on which both CS6 and STp or STh are located. 相似文献
959.
Andrew Ndegwa Makanya Ivanka Dimova Tobias Koller Beata Styp-Rekowska Valentin Djonov 《PloS one》2016,11(4)
The chick chorioallantoic membrane (CAM) is a widely used model for the study of angiogenesis, tumour growth, as well as drug efficacy. In spite of this, little is known about the developmental alteration from its appearance to the time of hatching. In the current study the CAM has been studied by classical stereology and allometry. Expression levels of selected angiogenesis-related molecules were estimated by RT-PCR and cell dynamics assessed by proliferation and apoptosis assays. Absolute CAM volume increased from a low of 0.47 ± 0.11 cm3 at embryonic day 8 (E8) to a high of 2.05 ± 0.27 cm3 at E18, and then decreased to 1.6 ± 0.47 cm3 at E20. On allometric analysis, three growth phases were identifiable. Between E8-13 (phase I), the CAM grew fastest; moderately in phase II (E13-18) but was regressing in phase III (E18-20). The chorion, the mesenchyme and the allantoic layers grew fastest in phase I, but moderately in phase II. The mesenchyme grew slowly in phase III while the chorion and allantois were regressing. Chorionic cell volume increased fastest in phase I and was regressing in phase III. Chorionic capillaries grew steadily in phase I and II but regressed in phase III. Both the chorion and the allantois grew by intrinsic cell proliferation as well as recruitment of cells from the mesenchyme. Cell proliferation was prominent in the allantois and chorion early during development, declined after E17 and apoptosis started mainly in the chorion from E14. VEGFR2 expression peaked at E11 and declined steadily towards E20, VEGF peaked at E13 and E20 while HIF 1α had a peak at E11 and E20. Studies targeting CAM growth and angiogenesis need to take these growth phases into consideration 相似文献
960.
Maternal effects are ubiquitous in nature and affect a wide range of offspring phenotypes. Recent research suggests that maternal effects also contribute to ageing, but the theoretical basis for these observations is poorly understood. Here we develop a simple model to derive expectations for (i) if maternal effects on ageing evolve; (ii) the strength of maternal effects on ageing relative to direct environmental effects; and (iii) the predicted relationships between environmental quality, maternal age and offspring lifespan. Our model is based on the disposable soma theory of ageing, and the key assumption is thus that mothers trade off their own somatic maintenance against investment in offspring. This trade-off affects the biological age of offspring at birth in terms of accumulated damage, as indicated by biomarkers such as oxidative stress or telomere length. We find that the optimal allocation between investment in maternal somatic investment and investment in offspring results in old mothers and mothers with low resource availability producing offspring with reduced life span. Furthermore, the effects are interactive, such that the strongest maternal age effects on offspring lifespan are found under low resource availability. These findings are broadly consistent with results from laboratory studies investigating the onset and rate of ageing and field studies examining maternal effects on ageing in the wild. 相似文献