The outer solar system: Perspectives for exobiology

The outer solar system contains many environments of interest for studies of the origin of life. Recent observations support the idea that Jupiter and Saturn have retained the mixture of elements originally present in the solar nebula. Subsequent low temperature chemistry has produced the expected array of simple molecules giving characteristic absorption bands in the spectra of these planets. Microwave and infrared observations show that the lower atmospheres are at temperatures above 300 K. Sources of energy for non-equilibrium chemistry seem available at least on Jupiter and the presence of an array of colored materials in the Jovian cloud belts has often been cited as evidence for the existence of complex abiogenic organic molecules. Further study of both planets in an exobiological context seems well worthwhile; potentially productive methods of investigation (including planned space missions) can be described and evaluated from this point of view. Uranus and Neptune are clearly deficient in light gases, but otherwise little is known with certainty about these distant planets. Again unusually high temperatures have been reported, but not above 273 K. Pluto and many of the outer planet satellites appear to represent a class of small bodies very unlike our neighbors in the inner solar system. Titan, Saturn's largest satellite, is especially interesting for our purposes because of its atmosphere. Methane and hydrogen are both present, and Titan's unusually reddish color again suggests the presence of organic compounds. The hydrogen-methane ratio is likely to be more similar to that of a primitive reducing terrestrial atmosphere than the ratios for Jupiter and Saturn, suggesting that in some respects this satellite may provide an even better model for early organic synthesis on the Earth. The problem of Titan's heat balance and atmospheric composition are currently under active investigation.     

A procedure for the synthesis of uridine-5'-diphospho-N-acetylglucosamine-14C

A complete procedure for the synthesis of 1-¹⁴C-glucosamine-labeled UDP-N-acetylglucosamine is described. Glucosamine is first phosphorylated with ATP and hexokinase to form glucosamine 6-phosphate. This is N-acetylated with acetic anhydride, and the product is converted to UDP-N-acetylglucosamine by incubation with a crude yeast extract. The sugar nucleotide is isolated from the incubation mixture by paper electrophoresis, and purified by paper chromatography.     

Nonsteady-State Three Compartment Tracer Kinetics: II. Sodium Flux Transients in the Toad Urinary Bladder in Response to Short Circuit

The theoretical approach presented in the previous paper provides an analytical method for determining the unidirectional, nonsteady-state fluxes in a three compartment system. Based on this a study was made of the sodium flux transients in the toad urinary bladder. A transient time-dependent state was generated by suddenly short-circuiting a bladder previously maintained in an open-circuited steady state. The sequence of experiments suggested by the theory provided the data required for the analysis. The results of these tracer experiments were consistent with the complex non-three compartmental structure of this tissue. As a result both of the inadequacy of the three compartment model in representing the tissue and of certain experimental difficulties, attempts at a quantitative solution were not entirely successful. Useful information was nevertheless obtained through a careful use of this model, and a qualitative analysis implied that the sodium influxes into the tissue at both of its surfaces are sensitive to changes in electrical potential while both effluxes are insensitive to this change. This suggests that both of the effluxes result from active processes while both influxes are associated with passive processes. The net transepithelial transport of sodium would then necessarily result from a more complex polarization than that proposed by Koefoed-Johnsen and Ussing.