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931.
Wessam Akel Patrick Thorwarth Vilson Mirdita Elmar A. Weissman Guozheng Liu Tobias Würschum C. Friedrich H. Longin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(4):973-984
Key message
Spelt wheat is a distinct genetic group to elite bread wheat, but heterosis for yield and protein quality is too low for spelt to be recommended as heterotic group for hybrid breeding in wheat.Abstract
The feasibility to switch from line to hybrid breeding is currently a hot topic in the wheat community. One limitation seems to be the lack of divergent heterotic groups within wheat adapted to a certain region. Spelt wheat is a hexaploid wheat that can easily be crossed with bread wheat and that forms a divergent genetic group when compared to elite bread wheat. The aim of this study was to investigate the potential of Central European spelt as a heterotic group for Central European bread wheat. We performed two large experimental field studies comprising in total 43 spelt lines, 14 wheat lines, and 273 wheat–spelt hybrids, and determined yield, heading time, plant height, resistance against yellow rust, leaf rust, and powdery mildew, as well as protein content and sedimentation volume. Heterosis of yield was found to be lower than that of hybrids made between elite wheat lines. Moreover, heterosis of the quality trait sedimentation volume was negative. Consequently, spelt wheat does not appear suited to be used as heterotic group in hybrid wheat breeding. Nevertheless, high combining abilities of a few spelt lines with elite bread wheat lines make them interesting resources for pre-breeding in bread wheat. Thereby, the low correlation between line per se performance and combining ability of these spelt lines shows the potential to unravel the breeding value of genetic resources by crossing them to an elite tester.932.
933.
Chie Shibazaki Shigeki Arai Rumi Shimizu Morihisa Saeki Takayoshi Kinoshita Andreas Ostermann Tobias E. Schrader Yuzuru Kurosaki Tomoko Sunami Ryota Kuroki Motoyasu Adachi 《Journal of molecular biology》2018,430(24):5094-5104
Casein kinase 2 (CK2) has broad phosphorylation activity against various regulatory proteins, which are important survival factors in eukaryotic cells. To clarify the hydration structure and catalytic mechanism of CK2, we determined the crystal structure of the alpha subunit of human CK2 containing hydrogen and deuterium atoms using joint neutron (1.9 Å resolution) and X-ray (1.1 Å resolution) crystallography. The analysis revealed the structure of conserved water molecules at the active site and a long potential hydrogen bonding network originating from the catalytic Asp156 that is well known to enhance the nucleophilicity of the substrate OH group to the γ-phospho group of ATP by proton elimination. His148 and Asp214 conserved in the protein kinase family are located in the middle of the network. The water molecule forming a hydrogen bond with Asp214 appears to be deformed. In addition, mutational analysis of His148 in CK2 showed significant reductions by 40%–75% in the catalytic efficiency with similar affinity for ATP. Likewise, remarkable reductions to less than 5% were shown by corresponding mutations on His131 in death-associated protein kinase 1, which belongs to a group different from that of CK2. These findings shed new light on the catalytic mechanism of protein kinases in which the hydrogen bond network through the C-terminal domain may assist the general base catalyst to extract a proton with a link to the bulk solvent via intermediates of a pair of residues. 相似文献
934.
935.
936.
Protein‐engineering of chitosanase from Bacillus sp. MN to alter its substrate specificity
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Eva K. Regel Tobias Weikert Anna Niehues Bruno M. Moerschbacher Ratna Singh 《Biotechnology and bioengineering》2018,115(4):863-873
Partially acetylated chitosan oligosaccharides (paCOS) have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities. One method to produce paCOS is partial chemical hydrolysis of chitosan polymers, but that leads to poorly defined mixtures of oligosaccharides. However, the effective production of defined paCOS is crucial for fundamental research and for developing applications. A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products. Protein‐engineering of these enzymes to alter their substrate specificity can overcome the limitations associated with naturally occurring enzymes and expand the spectrum of specific paCOS that can be produced. Here, engineering the substrate specificity of Bacillus sp. MN chitosanase is described for the first time. Two muteins with active site substitutions can accept N‐acetyl‐D‐glucosamine units at their subsite (?2), which is impossible for the wildtype enzyme. 相似文献
937.
938.
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super‐resolution microscopy
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Katharina J Seitz Martin S Helm Deblina Sarkar Rebecca S Saleeb Elisa D'Este Jessica Eberle Eva Wagner Christian Vogl Diana F Lazaro Frank Richter Javier Coy‐Vergara Giovanna Coceano Edward S Boyden Rory R Duncan Stefan W Hell Marcel A Lauterbach Stephan E Lehnart Tobias Moser Tiago F Outeiro Peter Rehling Blanche Schwappach Ilaria Testa Bolek Zapiec Silvio O Rizzoli 《The EMBO journal》2018,37(1):139-159
Paraformaldehyde (PFA) is the most commonly used fixative for immunostaining of cells, but has been associated with various problems, ranging from loss of antigenicity to changes in morphology during fixation. We show here that the small dialdehyde glyoxal can successfully replace PFA. Despite being less toxic than PFA, and, as most aldehydes, likely usable as a fixative, glyoxal has not yet been systematically tried in modern fluorescence microscopy. Here, we tested and optimized glyoxal fixation and surprisingly found it to be more efficient than PFA‐based protocols. Glyoxal acted faster than PFA, cross‐linked proteins more effectively, and improved the preservation of cellular morphology. We validated glyoxal fixation in multiple laboratories against different PFA‐based protocols and confirmed that it enabled better immunostainings for a majority of the targets. Our data therefore support that glyoxal can be a valuable alternative to PFA for immunostaining. 相似文献
939.
Selenium Utilization by GPX4 Is Required to Prevent Hydroperoxide-Induced Ferroptosis 总被引:1,自引:0,他引:1
Irina Ingold Carsten Berndt Sabine Schmitt Sebastian Doll Gereon Poschmann Katalin Buday Antonella Roveri Xiaoxiao Peng Florencio Porto Freitas Tobias Seibt Lisa Mehr Michaela Aichler Axel Walch Daniel Lamp Martin Jastroch Sayuri Miyamoto Wolfgang Wurst Fulvio Ursini Marcus Conrad 《Cell》2018,172(3):409-422.e21
940.
A three‐component system incorporating Ppd‐D1, copy number variation at Ppd‐B1, and numerous small‐effect quantitative trait loci facilitates adaptation of heading time in winter wheat cultivars of worldwide origin
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Tobias Würschum C. Friedrich H. Longin Matthew R. Tucker Willmar L. Leiser 《Plant, cell & environment》2018,41(6):1407-1416
The broad adaptability of heading time has contributed to the global success of wheat in a diverse array of climatic conditions. Here, we investigated the genetic architecture underlying heading time in a large panel of 1,110 winter wheat cultivars of worldwide origin. Genome‐wide association mapping, in combination with the analysis of major phenology loci, revealed a three‐component system that facilitates the adaptation of heading time in winter wheat. The photoperiod sensitivity locus Ppd‐D1 was found to account for almost half of the genotypic variance in this panel and can advance or delay heading by many days. In addition, copy number variation at Ppd‐B1 was the second most important source of variation in heading, explaining 8.3% of the genotypic variance. Results from association mapping and genomic prediction indicated that the remaining variation is attributed to numerous small‐effect quantitative trait loci that facilitate fine‐tuning of heading to the local climatic conditions. Collectively, our results underpin the importance of the two Ppd‐1 loci for the adaptation of heading time in winter wheat and illustrate how the three components have been exploited for wheat breeding globally. 相似文献