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31.
Pyrrolo-C (PC), or 3-[beta-D-2-ribofuranosyl]-6-methylpyrrolo[2,3-d]pyrimidin-2(3H)-one, is a fluorescent analog of the nucleoside cytidine that retains its Watson-Crick base-pairing capacity with G. Due to its red-shifted absorbance, it can be selectively excited in the presence of natural nucleosides, making it a potential site-specific probe for RNA structure and dynamics. Similar to 2-aminopurine nucleoside, which base-pairs with uridine (or thymidine), PC's fluorescence becomes reversibly quenched upon base-pairing, most likely due to stacking interactions with neighboring bases. To test its utility as an RNA probe, we examined PC's fluorescent properties over a wide range of ionic strengths, pH, organic cosolvents, and temperatures. Incorporation of PC into a single-stranded RNA results in an approximately 60% reduction of fluorescence intensity, while duplex formation reduces the fluorescence by approximately 75% relative to the free ribonucleoside. We find that the fluorescence intensity of PC is only moderately affected by ionic strength, pH, and temperature, while it is slightly enhanced by organic cosolvents, making it a versatile probe for a broad range of buffer conditions. We demonstrate two applications for PC: fluorescent measurements of the kinetics of formation and dissociation of an RNA/DNA complex, and fluorescent monitoring of the thermal denaturation of the central segment of an RNA duplex. Taken together, our data showcase the potential of pyrrolo-C as an effective fluorescent probe to study RNA structure, dynamics, and function, complementary to the popular 2-aminopurine ribonucleoside. 相似文献
32.
The hepatitis delta virus (HDV), an infectious human pathogen affecting millions of people worldwide, leads to intensified disease symptoms, including progression to liver cirrhosis upon coinfection with its helper virus, HBV. Both the circular RNA genome of HDV and its complementary antigenome contain a common cis-cleaving catalytic RNA motif, the HDV ribozyme, which plays a crucial role in viral replication. Previously, the crystal structure of the product form of the cis-acting genomic HDV ribozyme has been determined, and the precursor form has been suggested to be structurally similar. In contrast, solution studies by fluorescence resonance energy transfer (FRET) on a trans-cleaving form of the ribozyme have shown significant global conformational changes upon catalysis, while 2-aminopurine (AP) fluorescence assays have detected concomitant local conformational changes in the catalytic core. Here, we augment these studies by using terbium(III) to probe the structure of the trans-acting HDV ribozyme at nucleotide resolution. We observe significant structural differences between the precursor and product forms, especially in the P1.1 helix and the trefoil turn in the single-stranded region connecting P4 and P2 (termed J4/2) of the catalytic core. We show, using terbium(III) footprinting and sensitized luminescence spectroscopy as well as steady-state, time-resolved, and gel-mobility FRET assays on a systematic set of substrates, that the substrate sequence immediately 5' to the cleavage site significantly modulates these local as well as resultant global structural differences. Our results suggest a structural basis for the previously observed impact of the 5' substrate sequence on catalytic activity. 相似文献
33.
Finley DR Bell MG Borel AG Bloomquist WE Cohen ML Heiman ML Kriauciunas A Matthews DP Miles T Neel DA Rito CJ Sall DJ Shuker AJ Stephens TW Tinsley FC Winter MA Jesudason CD 《Bioorganic & medicinal chemistry letters》2006,16(21):5691-5694
The synthesis and biological evaluation of a series of benzimidazolone beta(3) adrenergic receptor agonists are described. A trend toward the reduction of rat atrial tachycardia upon increasing steric bulk at the 3-position of the benzimidazolone moiety was observed. 相似文献
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35.
Ojano-Dirain C Tinsley NB Wing T Cooper M Bottje WG 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2007,147(4):934-941
Increased hydrogen peroxide (H2O2) production was observed in duodenal mitochondria obtained from broiler chickens with low feed efficiency (FE). As a decrease in mitochondrial membrane potential (Deltapsi(m)) due to mild uncoupling of oxidative phosphorylation reduces reactive oxygen species production, this study was conducted to evaluate the effect of uncoupling on Deltapsi(m) and H2O2 production in duodenal mitochondria isolated from broilers with low (0.48+/-0.02) and high (0.68+/-0.01) FE. Membrane potential and H2O2 production were measured fluorometrically and in the presence of different levels of an uncoupler, carbonylcyanide-p-trifluoromethoxyphenylhydrazone (FCCP). The Deltapsi(m) was higher (P相似文献
36.
p150Glued, the largest subunit of the dynactin complex, is nonessential in Neurospora but required for nuclear distribution. 总被引:6,自引:2,他引:4 下载免费PDF全文
Dynactin is a multisubunit complex that is required for cytoplasmic dynein, a minus-end-directed, microtubule-associated motor, to efficiently transport vesicles along microtubules in vitro. p150Glued, the largest subunit of dynactin, has been identified in vertebrates and Drosophila and recently has been shown to interact with cytoplasmic dynein intermediate chains in vitro. The mechanism by which dynactin facilitates cytoplasmic dynein-dependent vesicle transport is unknown. We have devised a genetic screen for cytoplasmic dynein/dynactin mutants in the filamentous fungus Neurospora crassa. In this paper, we report that one of these mutants, ro-3, defines a gene encoding an apparent homologue of p150Glued, and we provide genetic evidence that cytoplasmic dynein and dynactin interact in vivo. The major structural features of vertebrate and Drosophila p150Glued, a microtubule-binding site at the N-terminus and two large alpha-helical coiled-coil regions contained within the distal two-thirds of the polypeptide, are conserved in Ro3. Drosophila p150Glued is essential for viability; however, ro-3 null mutants are viable, indicating that dynactin is not an essential complex in N. crassa. We show that N. crassa cytoplasmic dynein and dynactin mutants have abnormal nuclear distribution but retain the ability to organize cytoplasmic microtubules and actin in anucleate hyphae. 相似文献
37.
R. C. Tinsley 《Journal of Zoology》1973,169(1):1-27
A new species of African clawed toad, Xenopus kigesiensis sp.n., has been recorded from Lake Mutanda in South-west Kigezi, Uganda. Information on the biology and morphological variation of the species has been compiled from a sample preserved in the field and from a collection of living specimens maintained in the laboratory. An analysis of the characters of the species has been conducted and its relationships with the six known species of the genus assessed.
In the early part of this century the clawed toad populations occupying the lakes of South-West Kigezi exclusively comprised a Xenopus laevis subspecies. It appears that X. kigesiensis sp.n. has recently migrated into Lake Mutanda and has replaced the previous form. Hybridization experiments have demonstrated the reproductive isolation of X. kigesiensis sp.n. from two subspecies of X. laevis. There is evidence of a change in the ecology of the lakes during the period of apparent invasion.
The locality of X. kigesiensis sp.n. lies close to one of the main watersheds of Central Africa; taxonomically the toad forms an important link in the series of known Xenopus species. 相似文献
In the early part of this century the clawed toad populations occupying the lakes of South-West Kigezi exclusively comprised a Xenopus laevis subspecies. It appears that X. kigesiensis sp.n. has recently migrated into Lake Mutanda and has replaced the previous form. Hybridization experiments have demonstrated the reproductive isolation of X. kigesiensis sp.n. from two subspecies of X. laevis. There is evidence of a change in the ecology of the lakes during the period of apparent invasion.
The locality of X. kigesiensis sp.n. lies close to one of the main watersheds of Central Africa; taxonomically the toad forms an important link in the series of known Xenopus species. 相似文献
38.
Some effects of nickel toxicity on rye grass 总被引:3,自引:0,他引:3
Summary Rye grass (Lolium perenne, cv.S-23) was grown for 4 weeks in a non-calcareous Seaton loam soil with varying amounts of Ni as NiSO4. The purpose of this investigation was to study the Ni toxicity and the relationship of Ni with other essential elements. Nickel depressed shoot yield at all levels except at the lowest levelviz 30 g Ni/g soil. Nickel concentration of 50 g/g in shoots did not reduce the dry matter production in rye grass although slight chlorosis did appear at this level. The Ni and Fe concentration of the shoots increased and that of Mn and Zn decreased with increasing rates of Ni application. Uptake of Mn and Zn decreased at all level of Ni. But Fe uptake showed a slight increase at the first two levels and a profound depression at the subsequent levels. The pattern of Ni uptake is different, being highest at the middle level and decreasing on both sides which showed that the increase of Ni concentration of shoots is not proportional to the reduction in the yield. The Ni–Fe ratio rather than Ni and Fe concentration in plants has shown better relationship with the toxic effects of Ni. The implications of Ni phytotoxicity are discussed with particular reference to serpentine soils.The work is a part of Ph.D. Thesis submitted to the University of Aberdeen, U.K. 相似文献
39.
40.
Restriction-map variation associated with the G6PD polymorphism in natural populations of Drosophila melanogaster 总被引:10,自引:0,他引:10
Restriction-map variation was studied in 126 copies of the G6pd region in X
chromosome lines of Drosophila melanogaster from North America, Europe, and
Africa. Special attention was focused on the distribution of variation
relative to the geographically variable polymorphism for two
electrophoretic variants. Nucleotide heterozygosity as determined by eight
six-cutter restriction enzymes for the 13-kb region is estimated, on the
basis of the worldwide sample, to be 0.065%, which is the lowest value
reported for any comparable region in the D. melanogaster genome.
Significant linkage disequilibrium between electrophoretic alleles and
restriction-site variation is observed for several sites. In contrast to
published studies of other genetic regions, there are large insertions that
reach significant frequencies and are found across considerable geographic
distances. There is a clustering of this variation inside the first large
intervening sequence of the G6PD gene.
相似文献