全文获取类型
收费全文 | 101451篇 |
免费 | 3647篇 |
国内免费 | 2170篇 |
出版年
2022年 | 550篇 |
2021年 | 981篇 |
2020年 | 761篇 |
2019年 | 869篇 |
2018年 | 1763篇 |
2017年 | 1638篇 |
2016年 | 3584篇 |
2015年 | 7336篇 |
2014年 | 7202篇 |
2013年 | 6986篇 |
2012年 | 6464篇 |
2011年 | 3544篇 |
2010年 | 3084篇 |
2009年 | 2999篇 |
2008年 | 1572篇 |
2007年 | 1460篇 |
2006年 | 1315篇 |
2005年 | 7239篇 |
2004年 | 5877篇 |
2003年 | 4002篇 |
2002年 | 1502篇 |
2001年 | 1377篇 |
2000年 | 599篇 |
1999年 | 1723篇 |
1998年 | 502篇 |
1992年 | 2002篇 |
1991年 | 2075篇 |
1990年 | 2128篇 |
1989年 | 2036篇 |
1988年 | 1984篇 |
1987年 | 1831篇 |
1986年 | 1638篇 |
1985年 | 1676篇 |
1984年 | 1099篇 |
1983年 | 848篇 |
1982年 | 472篇 |
1979年 | 1075篇 |
1978年 | 764篇 |
1977年 | 608篇 |
1976年 | 630篇 |
1975年 | 873篇 |
1974年 | 997篇 |
1973年 | 1010篇 |
1972年 | 956篇 |
1971年 | 929篇 |
1970年 | 821篇 |
1969年 | 830篇 |
1968年 | 737篇 |
1967年 | 752篇 |
1966年 | 586篇 |
排序方式: 共有10000条查询结果,搜索用时 109 毫秒
51.
52.
53.
M Kh Ga?nutdinov S I Mirmakhmudova N M Mullagalieva Ia Kh Turakulov 《Biulleten' eksperimental'no? biologii i meditsiny》1984,97(3):293-295
Addition of a thermostable cytoplasmic fraction leads to the uncoupling of oxidative phosphorylation of the mitochondria. In hyperthyrosis such an effect manifests itself more powerfully than in the control. Addition of the thermostable cytoplasmic fraction induces electrogenic phosphate transport via the mitochondrial membrane. In hyperthyrosis, the activity of the thermostable inducer of phosphate transport in the cytoplasm increases. The functioning of the phosphate cycle may be the cause of the uncoupling of oxidative phosphorylation of the mitochondria during the disease in question. 相似文献
54.
Thyroid hormones are potent, instantaneous, and reversible inhibitors of ethanol oxidation catalyzed by isozymes of class I and II human alcohol dehydrogenase (ADH). None of the thyroid hormones inhibits class III ADH. At pH 7.40 the apparent Ki values vary between 55 and 110 microM for triiodothyronine, 35 and greater than 200 microM for thyroxine, and 10 and 23 microM for triiodothyroacetic acid. The inhibition is of a mixed type toward both NAD+ and ethanol. The binding of the thyroid hormone triiodothyronine to beta 1 gamma 1 ADH is mutually exclusive with 1,10-phenanthroline, 4-methylpyrazole, and testosterone, identifying a binding site(s) for the thyroid hormones, which overlap(s) both the 1,10-phenanthroline site near the active site zinc atom and the testosterone binding site, the latter being a regulatory site on the gamma-subunit-containing isozymes and distinct from their catalytic site. The inhibition by thyroid hormones may have implications for regulation of ADH catalysis of ethanol and alcohols in the intermediary metabolism of dopamine, norepinephrine, and serotonin and in steroid metabolism. In concert with other hormonal regulators, e.g., testosterone, the rate of ADH catalysis is capable of being fine tuned in accord with both substrate and modulator concentrations. 相似文献
55.
56.
E Ortega Rincón J M Marchena J J García A Schmidt T Schulz I Malpica A B Rodríguez C Barriga H Michna H L?tzerich 《Journal of applied physiology》2001,91(3):1067-1072
Flow cytometer measurements were made of the basal variations in peripheral blood functional monocytes and granulocytes over the course of a training season (January to November) of a cycling team. Parallel determinations were made of plasma concentration of catecholamines (chromatography) and cortisol (RIA) in a search for neuroendocrine markers. The results showed the greatest phagocytic capacity to occur in the central months (March, May, and July), coinciding with the greatest number and highest level of competitive events with good correlation with a peak in epinephrine during these months (r(2) = 0.998 for monocytes and r(2) = 0.674 for granulocytes). No good correlations were found between phagocytosis and norepinephrine or cortisol. The highest values for phagocytosis and epinephrine concentration were found in May. These results suggest that blood epinephrine concentration could be a good neuroendocrine marker of sportspeople's phagocytic response. 相似文献
57.
58.
Siew Choo Lim Matthew W. Bowler Ting Feng Lai Haiwei Song 《Nucleic acids research》2012,40(21):11009-11022
Mutations in immunoglobulin µ-binding protein 2 (Ighmbp2) cause distal spinal muscular atrophy type 1 (DSMA1), an autosomal recessive disease that is clinically characterized by distal limb weakness and respiratory distress. However, despite extensive studies, the mechanism of disease-causing mutations remains elusive. Here we report the crystal structures of the Ighmbp2 helicase core with and without bound RNA. The structures show that the overall fold of Ighmbp2 is very similar to that of Upf1, a key helicase involved in nonsense-mediated mRNA decay. Similar to Upf1, domains 1B and 1C of Ighmbp2 undergo large conformational changes in response to RNA binding, rotating 30° and 10°, respectively. The RNA binding and ATPase activities of Ighmbp2 are further enhanced by the R3H domain, located just downstream of the helicase core. Mapping of the pathogenic mutations of DSMA1 onto the helicase core structure provides a molecular basis for understanding the disease-causing consequences of Ighmbp2 mutations. 相似文献
59.
Following treatment of hen erythrocyte nuclei with dimethyl 3,3'-dithiobispropionimidate, dimers between histones H1a, H1b, and H5 were extracted with 5% perchloric acid. They resolved electrophoretically into four sub-bands and these were identified by non-reducing/reducing gel electrophoresis. The H5-H5 homodimer species was purified by gel electrophoresis and was treated sequentially with BrCN and dithiothreitol. The pattern of resulting fragments indicates that cross-links were mainly formed between the COOH-terminal portions and at a significantly lower frequency between the COOH-terminal and the NH2-terminal portions. 相似文献
60.
H Vaer?y F Nyberg H Franzén L Terenius 《Biochemical and biophysical research communications》1987,148(1):24-30
Enzyme activity capable of converting the glycine-extended substance P precursor, substance P-Gly12, into substance P was purified from human cerebrospinal fluid. The conversion reaction was monitored by radioimmunoassay measurement of substance P formation. The chemical identity of the product was verified by reversed-phase HPLC. The enzyme reaction was stimulated by Cu(II) ion and ascorbic acid and inhibited by the presence of diethyldithiocarbamate. By HPLC molecular sieving, the major enzyme activity appeared as a protein of 26,000 molecular weight. 相似文献