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81.
Tao Wan Qingxiang Han Ling Xian Yu Cao Apudo A. Andrew Xiaojie Pan Wei Li Fan Liu 《PloS one》2016,11(11)
Reproductive allocation is a key process in the plant life cycle and aquatic plants exhibit great diversity in their reproductive systems. In the present study, we conduct a field investigation of three aquatic macrophytes: Stuckenia pectinata, Myriophyllum spicatum, and Potamogeton perfoliatus. Our results showed that widespread species, including S. pectinata and M. spicatum had greater plasticity in their allocation patterns in the form of increased sexual and asexual reproduction, and greater potential to set seeds and increase fitness in more eutrophic environments. P. perfoliatus also exhibited a capacity to adopt varied sexual reproductive strategies such as setting more offspring for the future, although only in clear conditions with low nutrient levels. Our results establish strategies and mechanisms of some species for tolerating and surviving in varied eutrophic lake conditions. 相似文献
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Maternal BMI,gestational diabetes,and weight gain in relation to childhood obesity: The mediation effect of placental weight 下载免费PDF全文
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Zhe-Xin Li Shui-Gen Li Li-feng Zhang Su-ying Han Wan-Feng Li Hai-yan Xu Wen-hua Yang Ya-li Liu Yan-ru Fan Li-Wang Qi 《Plant Cell, Tissue and Organ Culture》2016,127(2):461-473
Somatic embryo (SE) regeneration is an ideal experimental system to realize rapid propagation of excellent clones and genetic improvement for perennial gymnosperms. In the present study, genes encoding the miRNA166 precursor were identified and LamiR166a was successfully transformed into the gymnosperm Larix leptolepis (L. leptolepis) and five LamiR166a over-expressed embryonic cell lines were screened out as stable embryo masses. As expected, the targets of miR166a, LaHDZ31-34, were all down-regulated in transgenic lines according to qRT-PCR results. The results showed that the percentage of normal SEs with 4–7 cotyledons was 77.0?% in wild type (WT) lines, but was reduced to 60.3?% in the pSuper::MIR166a lines with “cup-shaped” embryos comprised 7.0?% of WT and 20.7?% of transgenic embryos. Microscopic observation further showed that the intermediate region surrounded by the cotyledons was larger than in the control, with no upward bulge of the shoot apical meristem (SAM). The expression pattern of the two meristem marker genes CLAVATA (CLV) and WUSCHEL-related homeobox (WOX) were investigated. The results showed that the expression levels of WOX were three times higher in transgenic lines than in WT samples, which suggest that miR166a may indirectly regulate SAM development by directly affecting WOX expression. Besides, overexpression of LamiR166a clearly increased the rooting rate and promoted lateral root formation in L. leptolepis seedlings. These results may provide new insights into the regulatory role of miR166 in gymnosperms, and also new applications for forestry production in practice. 相似文献
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Cells contain a large number of metalloproteins that commonly harbor at least one metal ion cofactor. In metalloproteins, metal ions are usually coordinated by oxygen, sulfur, or nitrogen centers belonging to amino acid residues in the protein. The presence of the metal ion in metalloproteins allows them to take part in diverse biological processes, such as genome stability, metabolic catalysis, and cell cycle progression. Clinically, alteration of the function of metalloproteins in mammals is genetically associated with diseases characterized by DNA damage and repair defects. The present review focuses on the current perspectives of metal ion homeostasis in different organisms and summarizes the most recent understanding on magnesium, copper, iron, and manganese-containing proteins and their functional involvement in the maintenance of genome stability. 相似文献