全文获取类型
收费全文 | 48711篇 |
免费 | 4427篇 |
国内免费 | 6042篇 |
出版年
2024年 | 120篇 |
2023年 | 670篇 |
2022年 | 1552篇 |
2021年 | 2714篇 |
2020年 | 1932篇 |
2019年 | 2397篇 |
2018年 | 2218篇 |
2017年 | 1609篇 |
2016年 | 2120篇 |
2015年 | 3196篇 |
2014年 | 3876篇 |
2013年 | 3978篇 |
2012年 | 4856篇 |
2011年 | 4211篇 |
2010年 | 2712篇 |
2009年 | 2465篇 |
2008年 | 2750篇 |
2007年 | 2433篇 |
2006年 | 2086篇 |
2005年 | 1863篇 |
2004年 | 1459篇 |
2003年 | 1351篇 |
2002年 | 1069篇 |
2001年 | 783篇 |
2000年 | 659篇 |
1999年 | 667篇 |
1998年 | 432篇 |
1997年 | 375篇 |
1996年 | 378篇 |
1995年 | 305篇 |
1994年 | 301篇 |
1993年 | 196篇 |
1992年 | 229篇 |
1991年 | 208篇 |
1990年 | 162篇 |
1989年 | 148篇 |
1988年 | 113篇 |
1987年 | 109篇 |
1986年 | 81篇 |
1985年 | 89篇 |
1984年 | 57篇 |
1983年 | 44篇 |
1982年 | 35篇 |
1981年 | 25篇 |
1980年 | 11篇 |
1979年 | 10篇 |
1977年 | 11篇 |
1974年 | 10篇 |
1973年 | 10篇 |
1971年 | 12篇 |
排序方式: 共有10000条查询结果,搜索用时 312 毫秒
91.
Increased 5-Hydroxytryptamine and Norepinephrine Release from Rat Brain Slices by the Red Sea Flatfish 总被引:1,自引:1,他引:0
The effect of the Red Sea flatfish toxin pardaxin was examined on K+-evoked and on basal release of either [3H]norepinephrine or [3H]5-hydroxytryptamine from preloaded rat cortical slices. The K+-induced release of the neurotransmitters was stimulated in a dose-related manner at concentrations ranging from 0.5 to 4 micrograms/ml. Basal release of the two transmitters was elevated to a lesser extent. Although the stimulation of evoked release was approximately equivalent for the two neurotransmitters, the response to 5-hydroxytryptamine was reversible whereas that of norepinephrine was not washed by 20 min of superfusion. The mechanisms involved in producing these actions of pardaxin are not known; however, they may be mediated by changes in electrolyte fluxes across the neuronal membranes. 相似文献
92.
93.
我们将三种乙型肝炎表面抗原的合成肽段在联结载体或不接载体的情况下,分别制备成4种免疫原对兔子进行了免疫。大部分兔子都产生了抗肽抗体。其中抗P_(122-48)(auw)与抗P_(122-148)(adw)都能与天然HBsAg反应,而抗P_(122-148)(adw)的抗HBs活力比文献报道的都高,通过结构分析表明:含有天然蛋白上免疫显性区域的免疫原是合成疫苗的理想选者。 相似文献
94.
用~(14)C-Pro和~3H_2-Tyr离体暗培养黄瓜子叶,发现细胞质、SECW和RCW中的Hyp/Pro和Idt/Tyr都随时间呈线性增加。这两种比值后两者高于前者,而两种比值增加速度之比在胞质部分最大、RCW中最小。表明在胞质和胞壁中都有Pro羟化和Tyr异联化过程,但羟化作用主要发生在胞质中,异联化主要在RCW中。 理化处理(高渗溶液和CHM)和放射性示踪证明胞质中存在HRGP库;它被分泌到胞壁后,先以离子键与壁结合,后转变为共价键与壁结合的伸展素。 相似文献
95.
Crassulacean Acid Metabolism and Crassulacean Acid Metabolism Modifications in Peperomia camptotricha 总被引:9,自引:6,他引:3
下载免费PDF全文
![点击此处可从《Plant physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Peperomia camptotricha, a tropical epiphyte from Mexico, shows variable forms of Crassulacean acid metabolism (CAM). Young leaves exhibit CAM-cycling, while mature leaves show an intermediate type of metabolism, between CAM and CAM-cycling, having approximately the same amount of nighttime gas exchange as daytime. Metabolism of young leaves appears independent of daylength, but mature leaves have a tendency toward more CAM-like metabolism under short days (8 hours). Large differences in the physical appearance of plants were found between those grown under short daylengths and those grown under long daylengths (14 hours). Some anatomical differences were also detected in the leaves. Water stress caused a switch to CAM in young and mature leaves, and as water stress increased, they shifted to CAM-idling. 相似文献
96.
Physiological and isotopic aspects of several Peperomia species were investigated. All but one species had C3-like stomatal behavior, in that stomata were open during the day and closed during the night. In these species, most atmospheric CO2 uptake occurred during the day. Concurrent with this stomatal behavior, there were Crassulacean acid metabolism-like acid fluctuations in most species. Carbon and hydrogen isotope ratios of cellulose nitrate from Peperomia reflect their physiological behavior. The δ13C values of cellulose nitrate from Peperomia species were similar to values observed in C3 plants and consistent with the daytime uptake of exogeneous CO2 via the C3 photosynthetic pathway. The δD values of cellulose nitrate from Peperomia species approach those of Crassulacean acid metabolism plants. These elevated δD values are caused by fractionations occurring during biochemical reactions and not as a consequence of water relations. 相似文献
97.
Yan Yongshan Qian Jin Xi Xiahui 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1985,70(5):529-535
Summary Two 30 g/ml BrdU-resistant sublines and two 60 g/ml BrdU-resistant sublines are induced from a Chinese hamster cell line Wg3h (HGPRT–) by one-step and two-step selections, respectively. By inoculating the cells into BrdU-free medium or by adding more BrdU into the culture medium for 26–27 h, it was found that the two BrdU-resistant sublines analysed have very clear sister chromatid differential (SCD) staining patterns. This indicates that some of the nuclear DNA of the BrdU-resistant cells incorporate with BrdU to reach a kinetic balance. Frequencies of sister chromatid exchange (SCE) of the resistant cells are twice to four times as high as those of the Wg3h cells, depending on which BrdU-resistant subline is analysed. The SCE frequencies of the resistant cells also increase with the BrdU concentration in the medium. Analysis of silver-stained nucleolar organizer regions (NORs) indicates that the NOR activity of three out of the four BrdU-resistant sublines is significantly suppressed, i.e., averages of the Ag-NOR number and number of the chromosomes bearing Ag-NORs per cell decrease significantly. The degree of suppression for different BrdU-resistant sublines may be quite different. The suppressed NOR activity of the resistant cells can gradually be restored when the cells are inoculated into BrdU-free medium, but the recovery speed is far lower than that of the Wg3h cells. The suppression of the NOR activity of the BrdU-resistant sublines should be due to BrdU toxicity. 相似文献
98.
99.
由开花前1—4天的向日葵子房中取出胚珠,在液体培养基上进行漂浮培养,诱导了未受精的卵细胞发育为单倍体的胚状体.亦诱导了珠被绒毡层产生胚状体。对两种胚状体的发生和发育过程及其形态发生特点作了显微观察与描述。 相似文献
100.
百合花粉母细胞间细胞融合期间腺苷三磷酸酶活性的细胞化学定位及其与染色质胞间转移的关系 总被引:3,自引:0,他引:3
用标准的磷酸铅沉淀的细胞化学方法,对百合花粉母细胞间染色质穿壁运动期间及其前后三个时期中的腺苷三磷酸酶(ATP 酶)活性进行了超微结构的定位。结果表明:(1)在穿壁前,ATP 酶活性主要定位于质膜、胞间连丝及细胞间隙;在内质网、高尔基体、质体和某些局部的基质(groundplasm)中,也表现有 ATP 酶活性反应的产物;但在染色质和核仁中,一般都没有这种反应。(2)在穿壁时,染色质从一个细胞穿壁转移到另一个相邻细胞,同时看到染色质和核仁内出现密集的 ATP 酶活性反应产物;在内质网和高尔基体的腔内以及质体的片层上也产生明显的 ATP 酶活性反应;而在质膜、胞间连丝及细胞间隙内 ATP 酶活性明显降低,甚至看不到明显的活性反应。(3)在穿壁后,质膜及细胞间隙中又产生明显的 ATP 酶活性反应产物,但核内染色质上的 ATP 酶活性则显著降低,而核仁内则仍有较高的活性。同前二个时期一样,内质网、高尔基体和质体上的 ATP 酶仍表现明显的活性反应。最后讨论了三个不同发育时期 ATP 酶活性及其分布部位的改变与染色质胞间转移的关系。 相似文献