全文获取类型
收费全文 | 13874篇 |
免费 | 1264篇 |
国内免费 | 1214篇 |
专业分类
16352篇 |
出版年
2024年 | 31篇 |
2023年 | 213篇 |
2022年 | 461篇 |
2021年 | 657篇 |
2020年 | 506篇 |
2019年 | 578篇 |
2018年 | 573篇 |
2017年 | 432篇 |
2016年 | 569篇 |
2015年 | 847篇 |
2014年 | 1024篇 |
2013年 | 1039篇 |
2012年 | 1237篇 |
2011年 | 1108篇 |
2010年 | 686篇 |
2009年 | 570篇 |
2008年 | 703篇 |
2007年 | 623篇 |
2006年 | 535篇 |
2005年 | 482篇 |
2004年 | 378篇 |
2003年 | 394篇 |
2002年 | 301篇 |
2001年 | 254篇 |
2000年 | 232篇 |
1999年 | 203篇 |
1998年 | 128篇 |
1997年 | 124篇 |
1996年 | 125篇 |
1995年 | 121篇 |
1994年 | 96篇 |
1993年 | 89篇 |
1992年 | 150篇 |
1991年 | 121篇 |
1990年 | 85篇 |
1989年 | 90篇 |
1988年 | 77篇 |
1987年 | 68篇 |
1986年 | 50篇 |
1985年 | 70篇 |
1984年 | 40篇 |
1983年 | 35篇 |
1982年 | 28篇 |
1981年 | 17篇 |
1979年 | 16篇 |
1978年 | 15篇 |
1977年 | 16篇 |
1973年 | 13篇 |
1971年 | 19篇 |
1970年 | 14篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
41.
Bovine lens cytoplasmic aldehyde dehydrogenase exhibits Michaelis-Menten kinetics with acetaldehyde, glyceraldehyde 3-phosphate, p-nitrobenzaldehyde, propionaldehyde, glycolaldehyde, glyceraldehyde, phenylacetylaldehyde and succinic semialdehyde as substrates. The enzyme was also active with malondialdehyde, and exhibited an esterase activity. Steady-state kinetic analyses show that the enzyme exhibits a compulsory-ordered ternary-complex mechanism with NAD+ binding before acetaldehyde. The enzyme was inhibited by disulfiram and by p-chloromercuribenzoate, and studies with with mercaptans indicated the involvement of thiol groups in catalysis. 相似文献
42.
43.
44.
45.
46.
47.
玉米花粉单倍体植株染色体上异染色质的变异 总被引:4,自引:1,他引:3
我们用Giemsa BSG C-带技术检查了玉米花药培养获得的花粉单倍体植株根尖细胞染色体上异染色质的变异,观察结果表明,有的植株所显示的C-带数目是与供体植株的相一致,有的植株所显示的C-带数目则发生了显著变化,其中有的增加,有的减少。并讨论了异染色质发生变异的可能原因。还相应地观察到间期核中染色中心的变化是与中期染色体上C-带数目的变化相一致。 相似文献
48.
49.
C K Chou T S Su C M Chang C P Hu M Y Huang C S Suen N W Chou L P Ting 《The Journal of biological chemistry》1989,264(26):15304-15308
The human hepatoma Hep3B cells contain integrated hepatitis B viral genome and continually secret hepatitis B surface antigen (HBsAg). The production of HBsAg (but not alpha-fetoprotein) was suppressed by addition of low concentrations (0.1-1 nM) of insulin into serum-free medium. In addition, the suppression of HBsAg production by insulin was paralleled with the decrease in HBsAg mRNA abundance. Insulin also cause a rapid rate of disappearance of HBsAg mRNA (t 1/2, 2 h) in Hep3B cells. The Hep3B cells carry specific receptor with high affinity for insulin (Kd = 1.8 nM). The receptor showed an insulin-dependent protein tyrosine kinase activity. The half-maximal insulin concentration for the activation of the receptor kinase was about 5 nM. Only very high concentrations of insulin-like growth factor I and human proinsulin can compete for the insulin receptor binding and suppress HBsAg production, this suggests that insulin may act through its receptor binding to suppress HBsAg expression in human hepatoma Hep3B cells. 相似文献
50.
Acetylcholine receptor in a C2 muscle cell variant is retained in the endoplasmic reticulum 总被引:12,自引:10,他引:2 下载免费PDF全文
We have examined the properties and intracellular localization of acetylcholine receptors in the C2 muscle cell line and in a variant (T-) that accumulates AChR intracellularly. On immunoblots, the subunit structures of the AChR from wild-type and T- cells were similar except that the gamma and delta subunits of the variant AChR had altered mobilities. Digestion with endoglycosidases H and F demonstrated that this difference results from a failure of high-mannose N-linked oligosaccharides on AChR subunits to be processed to complex forms in the variant. N-linked glycosylation of other proteins in the variant was normal. When examined by immunocytochemistry, the distribution of internal AChR in wild-type cells was consistent with a location both in the endoplasmic reticulum and in the Golgi. Variant cells, however, showed no evidence of Golgi staining. Subcellular fractionation experiments also demonstrated AChR in the Golgi fractions of wild-type cells, but not in those derived from T- cells. We conclude that in T- myotubes most of the AChR fails to be transported out of the endoplasmic reticulum. 相似文献