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排序方式: 共有138条查询结果,搜索用时 15 毫秒
71.
Timm Konold A Robin Sayers Amanda Sach Gemma E Bone Steven van Winden Gerald AH Wells Marion M Simmons Michael J Stack Angus Wear Steve AC Hawkins 《BMC veterinary research》2010,6(1):53
Background
Various clinical protocols have been developed to aid in the clinical diagnosis of classical bovine spongiform encephalopathy (BSE), which is confirmed by postmortem examinations based on vacuolation and accumulation of disease-associated prion protein (PrPd) in the brain. The present study investigated the occurrence and progression of sixty selected clinical signs and behaviour combinations in 513 experimentally exposed cattle subsequently categorised postmortem as confirmed or unconfirmed BSE cases. Appropriate undosed or saline inoculated controls were examined similarly and the data analysed to explore the possible occurrence of BSE-specific clinical expression in animals unconfirmed by postmortem examinations. 相似文献72.
73.
In diagnostic curettages of the uterine cavity wall in 14 women 878 glands have been investigated. The following parameters have been estimated: perimeters of the gland section contours and their lumens, areas of the gland sections, their lumens and epithelial rings, coefficients of the gland forms and their lumens, distance between glands. Peculiarities connected with physiological differences of the uterine cavity mucose membrane are revealed at various phases of the ovarian menstrual cycle 相似文献
74.
Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium
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Verónica Lloréns‐Rico Francis J O'Reilly Judith AH Wodke E Besray Unal Eva Yus Sira Martínez Robert J Nichols Tony Ferrar Ana Vivancos Arne Schmeisky Jörg Stülke Vera van Noort Anne‐Claude Gavin Peer Bork Luis Serrano 《Molecular systems biology》2015,11(1)
Identifying all essential genomic components is critical for the assembly of minimal artificial life. In the genome-reduced bacterium Mycoplasma pneumoniae, we found that small ORFs (smORFs; < 100 residues), accounting for 10% of all ORFs, are the most frequently essential genomic components (53%), followed by conventional ORFs (49%). Essentiality of smORFs may be explained by their function as members of protein and/or DNA/RNA complexes. In larger proteins, essentiality applied to individual domains and not entire proteins, a notion we could confirm by expression of truncated domains. The fraction of essential non-coding RNAs (ncRNAs) non-overlapping with essential genes is 5% higher than of non-transcribed regions (0.9%), pointing to the important functions of the former. We found that the minimal essential genome is comprised of 33% (269,410 bp) of the M. pneumoniae genome. Our data highlight an unexpected hidden layer of smORFs with essential functions, as well as non-coding regions, thus changing the focus when aiming to define the minimal essential genome. 相似文献
75.
76.
T. B. Kalinnikova A. Kh. Timoshenko T. M. Gainutdinov V. V. Gindina M. Kh. Gainutdinov 《Journal of Evolutionary Biochemistry and Physiology》2006,42(5):575-582
Action of high temperature (36°C) on the nematode Caenorhabditis elegans organism was manifested in errors of the motor program of swimming induced by a mechanical stimulus (37 ± 2 min), the complete, but reversible cessation of locomotion (57 ± 3 min), while damage—in thermal death (215 ± 5 min). Addition into medium of atropine (10?8–10?9 M) and chemical stimuli (10?8–10?6 cAMP or lysine) causes considerable changes of thermal stability of the worm locomotion. Analysis of these data has shown that the cause of the reversible thermal disturbance of the C. elegans locomotion is disintegration of neurons in the nervous centers regulating behavior. The obtained data indicate the presence in the simple organism of C. elegans of adaptations increasing stability of processes of integration of neurons to a high temperature, which were found earlier in arthropods and vertebrates. 相似文献
77.
Martijn AH Oude Voshaar Peter M ten Klooster Erik Taal Eswar Krishnan Mart AFJ van de Laar 《Arthritis research & therapy》2012,14(2):R47-7
Introduction
Patient-reported physical function is an established outcome domain in clinical studies in rheumatology. To overcome the limitations of the current generation of questionnaires, the Patient-Reported Outcomes Measurement Information System (PROMIS®) project in the USA has developed calibrated item banks for measuring several domains of health status in people with a wide range of chronic diseases. The aim of this study was to translate and cross-culturally adapt the PROMIS physical function item bank to the Dutch language and to pretest it in a sample of patients with arthritis.Methods
The items of the PROMIS physical function item bank were translated using rigorous forward-backward protocols and the translated version was subsequently cognitively pretested in a sample of Dutch patients with rheumatoid arthritis.Results
Few issues were encountered in the forward-backward translation. Only 5 of the 124 items to be translated had to be rewritten because of culturally inappropriate content. Subsequent pretesting showed that overall, questions of the Dutch version were understood as they were intended, while only one item required rewriting.Conclusions
Results suggest that the translated version of the PROMIS physical function item bank is semantically and conceptually equivalent to the original. Future work will be directed at creating a Dutch-Flemish final version of the item bank to be used in research with Dutch speaking populations. 相似文献78.
Svetlana Sarantseva Svetlana Timoshenko Olga Bolshakova Eugenia Karaseva Dmitry Rodin Alexander L. Schwarzman Michael P. Vitek 《PloS one》2009,4(12)
Background
Mutations of the amyloid precursor protein gene (APP) are found in familial forms of Alzheimer''s disease (AD) and some lead to the elevated production of amyloid-β-protein (Aβ). While Aβ has been implicated in the causation of AD, the exact role played by Aβ and its APP precursor are still unclear.Principal Findings
In our study, Drosophila melanogaster transgenics were established as a model to analyze AD-like pathology caused by APP overexpression. We demonstrated that age related changes in the levels and pattern of synaptic proteins accompanied progressive neurodegeneration and impairment of cognitive functions in APP transgenic flies, but that these changes may be independent from the generation of Aβ. Using novel peptide mimetics of Apolipoprotein-E, COG112 or COG133 proved to be neuroprotective and significantly improved the learning and memory of APP transgenic flies.Conclusions
The development of neurodegeneration and cognitive deficits was corrected by injections of COG112 or COG133, novel mimetics of apolipoprotein-E (apoE) with neuroprotective activities. 相似文献79.
80.
Shivani M Chidrawar Naeem Khan Y L Tracey Chan Laxman Nayak Paul AH Moss 《Immunity & ageing : I & A》2006,3(1):10-8