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221.
Jan-Uwe Schmidt Alexander Eilers Madlen Schimkat Jonas Krause-Heiber Andreas Timm Winfried Nachtigall 《Bird Study》2017,64(1):1-11
Capsule: Sky Lark plots and additional tramlines increase Sky Lark Alauda arvensis territory densities in winter crops.Aims: To analyse the effects on Sky Lark territory density of Sky Lark plots and additional tramlines in winter cereals and oilseed rape.Methods: We mapped Sky Lark territories on fields with Sky Lark plots or additional tramlines as well as on adjacent control sites, from 2010 to 2013 in Saxony, Germany, where agricultural land use is intensive, dominated by winter-sown crops and takes place in large fields.Results: In test sites with Sky Lark plots, 5.6 and 3.1 territories per 10?ha were found in the early (April/May) and late periods (June/July) respectively, compared to 3.3 and 1.4 territories per 10?ha in control sites. Sky Lark territory densities in fields with additional tramlines were 1.6 times higher in the early period (4.2 versus 2.6 territories per 10?ha) and 2.2 times higher in the late period (3.6 versus 1.6 territories per 10?ha).Conclusion: Sky Lark plots and additional tramlines improve large fields for the Sky Lark and have the potential to increase the Sky Lark population. 相似文献
222.
This study evaluated the effects of three climate scenarios predicted by the Intergovernmental Panel on Climate Change for 2100, on the growth and physiology of the tambaqui (Colossoma macropomum). Juveniles of tambaqui were reared in four rooms (current conditions, and the climate scenarios B1, A1B and A2) for 150 days. Twelve fish were regularly collected for blood analyses and to have their weight and length measured. The amount of food consumed over the experimental period was recorded. Feed intake, weight gain and food conversion showed an increase in all climate scenarios (P < 0.05). Although these results were expected, the increases in weight gain in the more dramatic environmental situations were due to increases in feed intake. Increases in haematocrit, glucose, cortisol and triglycerides within the first 30 days of the experiment were observed. The present results indicate that climate changes can affect not only its feed intake, but also the growth of the tambaqui and that this fish species can recover blood parameters, suggesting an artificial acclimatization to the analysed environmental conditions after 30 days. Taken to nature, these characteristics of the tambaqui could cause ecological imbalances in the food web, in its frequency of occurrence and in its dominance. 相似文献
223.
Kate Cronin Haylee Kaplan Mirijam Gaertner Ulrike M. Irlich M. Timm Hoffman 《Biological invasions》2017,19(3):925-937
The horticultural industry is recognised as a major pathway for the introduction and spread of invasive alien plants (IAPs). The Conservation of Agricultural Resources Act (CARA) of 1983 (Act No. 43 of 1983) listed and categorised invasive species with an aim to curb their spread. The more recently enacted Alien and Invasive Species Regulations under South Africa’s National Environmental Management: Biodiversity Act (NEMBA) are intended, in part, to improve controls on the horticultural industry’s role in the spread of IAPs. In order to assess, and enhance, the likely effectiveness of NEMBA, it is important to build an understanding of stakeholders’ awareness and attitudes towards the control of IAPs and associated regulatory policies. A two-pronged approach—involving nursery manager interviews (n = 30) and plant stock audit assessments (n = 41)—was used to gauge the awareness, compliance and attitudes of nursery managers towards both the CARA and NEMBA invasive species regulations. Less than ten percent of audited nurseries were fully compliant with the NEMBA regulations, and over 50% were stocking IAPs that have been regulated for at least 13 years under CARA. This is despite high levels of awareness (70%) about the CARA regulations reported in the interviews. The majority (73.5%) of IAP species stocked in nurseries were NEMBA category 1b invaders such as Nerium oleander and Canna indica. These are widespread and well-established invaders that require compulsory control under NEMBA. Half of the managers were not aware that the NEMBA regulations had been promulgated, but most respondents nevertheless reported being enthusiastic about compliance. Several factors were quoted as constraints on compliance by the industry. These included a perceived lack of enforcement, weak communication from government, and the lack of inclusion of the industry in the regulatory process. Suggested interventions that could enhance the impact of IAP regulations will involve improving the user-friendliness of the regulations, and supplementing the current top-down approach to regulation with an inclusive partner-centred approach. 相似文献
224.
Timm Konold Gemma Bone Alberto Vidal-Diez Raul Tortosa Andrew Davis Glenda Dexter Peter Hill Martin Jeffrey Marion M Simmons Melanie J Chaplin Susan J Bellworthy Christine Berthelin-Baker 《BMC veterinary research》2008,4(1):1-15
Background
The variability in the clinical or pathological presentation of transmissible spongiform encephalopathies (TSEs) in sheep, such as scrapie and bovine spongiform encephalopathy (BSE), has been attributed to prion protein genotype, strain, breed, clinical duration, dose, route and type of inoculum and the age at infection. The study aimed to describe the clinical signs in sheep infected with the BSE agent throughout its clinical course to determine whether the clinical signs were as variable as described for classical scrapie in sheep. The clinical signs were compared to BSE-negative sheep to assess if disease-specific clinical markers exist.Results
Forty-seven (34%) of 139 sheep, which comprised 123 challenged sheep and 16 undosed controls, were positive for BSE. Affected sheep belonged to five different breeds and three different genotypes (ARQ/ARQ, VRQ/VRQ and AHQ/AHQ). None of the controls or BSE exposed sheep with ARR alleles were positive. Pruritus was present in 41 (87%) BSE positive sheep; the remaining six were judged to be pre-clinically infected. Testing of the response to scratching along the dorsum of a sheep proved to be a good indicator of clinical disease with a test sensitivity of 85% and specificity of 98% and usually coincided with weight loss. Clinical signs that were displayed significantly earlier in BSE positive cases compared to negative cases were behavioural changes, pruritic behaviour, a positive scratch test, alopecia, skin lesions, teeth grinding, tremor, ataxia, loss of weight and loss of body condition. The frequency and severity of each specific clinical sign usually increased with the progression of disease over a period of 16–20 weeks.Conclusion
Our results suggest that BSE in sheep presents with relatively uniform clinical signs, with pruritus of increased severity and abnormalities in behaviour or movement as the disease progressed. Based on the studied sheep, these clinical features appear to be independent of breed, affected genotype, dose, route of inoculation and whether BSE was passed into sheep from cattle or from other sheep, suggesting that the clinical phenotype of BSE is influenced by the TSE strain more than by other factors. The clinical phenotype of BSE in the genotypes and breed studied was indistinguishable from that described for classical scrapie cases. 相似文献225.
226.
Merz F Boehringer D Schaffitzel C Preissler S Hoffmann A Maier T Rutkowska A Lozza J Ban N Bukau B Deuerling E 《The EMBO journal》2008,27(11):1622-1632
Ribosome-associated chaperone Trigger Factor (TF) initiates folding of newly synthesized proteins in bacteria. Here, we pinpoint by site-specific crosslinking the sequence of molecular interactions of Escherichia coli TF and nascent chains during translation. Furthermore, we provide the first full-length structure of TF associated with ribosome-nascent chain complexes by using cryo-electron microscopy. In its active state, TF arches over the ribosomal exit tunnel accepting nascent chains in a protective void. The growing nascent chain initially follows a predefined path through the entire interior of TF in an unfolded conformation, and even after folding into a domain it remains accommodated inside the protective cavity of ribosome-bound TF. The adaptability to accept nascent chains of different length and folding states may explain how TF is able to assist co-translational folding of all kinds of nascent polypeptides during ongoing synthesis. Moreover, we suggest a model of how TF's chaperoning function can be coordinated with the co-translational processing and membrane targeting of nascent polypeptides by other ribosome-associated factors. 相似文献
227.
Salloum S Oniangue-Ndza C Neumann-Haefelin C Hudson L Giugliano S aus dem Siepen M Nattermann J Spengler U Lauer GM Wiese M Klenerman P Bright H Scherbaum N Thimme R Roggendorf M Viazov S Timm J 《Journal of virology》2008,82(23):11803-11812
The inherent sequence diversity of the hepatitis C virus (HCV) represents a major hurdle for the adaptive immune system to control viral replication. Mutational escape within targeted CD8 epitopes during acute HCV infection has been well documented and is one possible mechanism for T-cell failure. HLA-B*08 was recently identified as one HLA class I allele associated with spontaneous clearance of HCV replication. Selection of escape mutations in the immunodominant HLA-B*08-restricted epitope HSKKKCDEL(1395-1403) was observed during acute infection. However, little is known about the impact of escape mutations in this epitope on viral replication capacity. Their previously reported reversion back toward the consensus residue in patients who do not possess the B*08 allele suggests that the consensus sequence in this epitope is advantageous for viral replication in the absence of immune pressure. The aim of this study was to determine the impact of mutational escape from this immunodominant epitope on viral replication. We analyzed it with a patient cohort with chronic HCV genotype 1b infection and in a single-source outbreak (genotype 1b). Sequence changes in this highly conserved region are rare and selected almost exclusively in the presence of the HLA-B*08 allele. When tested in the subgenomic replicon (Con1), the observed mutations reduce viral replication compared with the prototype sequence. The results provide direct evidence that escape mutations in this epitope are associated with fitness costs and that the antiviral effect of HLA-B*08-restricted T cells is sufficiently strong to force the virus to adopt a relatively unfavorable sequence. 相似文献
228.
229.
Kettering M Sterner O Anke T 《Zeitschrift für Naturforschung. C, Journal of biosciences》2004,59(11-12):816-823
In dual cultures Oudemansiella mucida and Xerula melanotricha (basidiomycetes) react to the presence of living Penicillium notatum or P. turbatum with an increased production of strobilurin A (1) or X (2). P. notatum in turn reacts to the two basidiomycetes or their antibiotic strobilurin A alone with the production of N-(2-hydroxypropanoyl)-2-aminobenzoic acid amide (3) or chrysogine (4). P. melinii and P. urticae overgrow O. mucida due to complete resistance to strobilurin A. P. brevicompactum, P. citrinum, P. janczewskii and the other Penicillium strains are all sensitive but apparently do not induce O. mucida to produce the amounts of strobilurin A needed to inhibit their growth. 相似文献
230.
Timm DE Baker LJ Mueller H Zidek L Novotny MV 《Protein science : a publication of the Protein Society》2001,10(5):997-1004
The mouse major urinary proteins are pheromone-binding proteins that function as carriers of volatile effectors of mouse physiology and behavior. Crystal structures of recombinant mouse major urinary protein-I (MUP-I) complexed with the synthetic pheromones, 2-sec-butyl-4,5-dihydrothiazole and 6-hydroxy-6-methyl-3-heptanone, have been determined at high resolution. The purification of MUP-I from mouse liver and a high-resolution structure of the natural isolate are also reported. These results show the binding of 6-hydroxy-6-methyl-3-heptanone to MUP-I, unambiguously define ligand orientations for two pheromones within the MUP-I binding site, and suggest how different chemical classes of pheromones can be accommodated within the MUP-I beta-barrel. 相似文献