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排序方式: 共有443条查询结果,搜索用时 31 毫秒
71.
Eva M. Jouaux Barbara B. Timm Katja M. Arndt Thomas E. Exner 《Journal of peptide science》2009,15(1):5-15
Previously, a Myc‐interfering peptide (Mip) was identified for the targeted inactivation of the Myc:Max complex by the combination of rational design and an in vivo protein‐fragment complementation assay. In the subsequent work presented here, molecular dynamics simulations and free energy calculations based on the molecular mechanics GBSA method were performed to define the contribution of the different amino acids in the Myc:Mip coiled coil domain, and compared to wild‐type Myc:Max. For further optimization of the Myc interference, point mutations were introduced into Mip and analyzed, from which two showed much higher binding affinities in the computational studies in good agreement with the experiment. These mutants with very high potential for inactivation of Myc can now be used as starting point for further optimizations based on the computational as well as experimental protocols presented here. Copyright © 2008 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
72.
Hendrik Wilking Mario Ziller Christoph Staubach Anja Globig Timm C. Harder Franz J. Conraths 《PloS one》2009,4(8)
Highly pathogenic influenza virus (HPAIV) H5N1 proved to be remarkably mobile in migratory bird populations where it has led to extensive outbreaks for which the true number of affected birds usually cannot be determined. For the evaluation of avian influenza monitoring and HPAIV early warning systems, we propose a time-series analysis that includes the estimation of confidence intervals for (i) the prevalence in outbreak situations or (ii) in the apparent absence of disease in time intervals for specified regional units. For the German outbreak regions in 2006 and 2007, the upper 95% confidence limit allowed the detection of prevalences below 1% only for certain time intervals. Although more than 25,000 birds were sampled in Germany per year, the upper 95% confidence limit did not fall below 5% in the outbreak regions for most of the time. The proposed analysis can be used to monitor water bodies and high risk areas, also as part of an early-warning system. Chances for an improved targeting of the monitoring system as part of a risk-based approach are discussed with the perspective of reducing sample sizes. 相似文献
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Urschel S Höher T Schubert T Alev C Söhl G Wörsdörfer P Asahara T Dermietzel R Weiler R Willecke K 《The Journal of biological chemistry》2006,281(44):33163-33171
Gap junctions in AII amacrine cells of mammalian retina participate in the coordination of the rod and cone signaling pathway involved in visual adaptation. Upon stimulation by light, released dopamine binds to D(1) receptors on AII amacrine cells leading to increased intracellular cAMP (cyclic adenosine monophosphate) levels. AII amacrine cells express the gap junctional protein connexin36 (Cx36). Phosphorylation of Cx36 has been hypothesized to regulate gap junctional activity of AII amacrine cells. However, until now in vivo phosphorylation of Cx36 has not been reported. Indeed, it had been concluded that Cx36 in bovine retina is not phosphorylated, but in vitro phosphorylation for Cx35, the bass ortholog of Cx36, had been shown. To clarify this experimental discrepancy, we examined protein kinase A (PKA)-induced phosphorylation of Cx36 in mouse retina as a possible mechanism to modulate the extent of gap junctional coupling. The cytoplasmic domains of Cx36 and the total Cx36 protein were phosphorylated in vitro by PKA. Mass spectroscopy revealed that all four possible PKA consensus motifs were phosphorylated; however, domains point mutated at the sites in question showed a prevalent usage of Ser-110 and Ser-293. Additionally, we demonstrated that Cx36 was phosphorylated in cultured mouse retina. Furthermore, activation of PKA increased the level of phosphorylation of Cx36. cAMP-stimulated, PKA-mediated phosphorylation of Cx36 protein was accompanied by a decrease of tracer coupling between AII amacrine cells. Our results link increased phosphorylation of Cx36 to down-regulation of permeability through gap junction channels mediating light adaptation in the retina. 相似文献
76.
Simultaneous stimulation of sedoheptulose 1,7‐bisphosphatase,fructose 1,6‐bisphophate aldolase and the photorespiratory glycine decarboxylase‐H protein increases CO2 assimilation,vegetative biomass and seed yield in Arabidopsis
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Andrew J. Simkin Patricia E. Lopez‐Calcagno Philip A. Davey Lauren R. Headland Tracy Lawson Stefan Timm Hermann Bauwe Christine A. Raines 《Plant biotechnology journal》2017,15(7):805-816
In this article, we have altered the levels of three different enzymes involved in the Calvin–Benson cycle and photorespiratory pathway. We have generated transgenic Arabidopsis plants with altered combinations of sedoheptulose 1,7‐bisphosphatase (SBPase), fructose 1,6‐bisphophate aldolase (FBPA) and the glycine decarboxylase‐H protein (GDC‐H) gene identified as targets to improve photosynthesis based on previous studies. Here, we show that increasing the levels of the three corresponding proteins, either independently or in combination, significantly increases the quantum efficiency of PSII. Furthermore, photosynthetic measurements demonstrated an increase in the maximum efficiency of CO2 fixation in lines over‐expressing SBPase and FBPA. Moreover, the co‐expression of GDC‐H with SBPase and FBPA resulted in a cumulative positive impact on leaf area and biomass. Finally, further analysis of transgenic lines revealed a cumulative increase of seed yield in SFH lines grown in high light. These results demonstrate the potential of multigene stacking for improving the productivity of food and energy crops. 相似文献
77.
Postel A Letzel T Müller F Ehricht R Pourquier P Dauber M Grund C Beer M Harder TC 《Analytical biochemistry》2011,(1):49-31
There is an urgent need for robust subtype-specific serological tests to diagnose influenza A virus infections in poultry and mammals, including humans. Such assays require reliable subtype-specific sources of soluble and authentically folded seroreactive hemagglutinin (HA), one of the integral membrane proteins that determine the serological subtype of influenza viruses. To this purpose, a bigenic pFastBacDual baculovirus transfer vector allowing efficient in vivo biotinylation of soluble HA homo-oligomers expressed via the secretory pathway was developed. An Avi-Tag allowed site-specific biotinylation by a coexpressed genetically modified BirA biotin ligase retained in the endoplasmic reticulum (ER). Highly seroreactive mono-biotinylated HA of recent H5 and H7 influenza A subtypes was secreted from recombinant baculovirus infected High-Five insect cells at levels sufficient to directly load streptavidin-coated enzyme-linked immunosorbent assay (ELISA) matrices, thereby avoiding any purification steps. The recombinant antigens retained authentic antigenicity, including conformation-dependent epitopes involved in hemagglutination inhibition as detected by monoclonal antibodies. This is the first bigenic in vivo biotinylation system established for use in insect cells with secretable recombinant membrane proteins biotinylated by an ER-retained variant of BirA biotin ligase. The proposed technique is expected to significantly increase flexibility in the design of subtype-specific assays, thereby expanding the power of influenza A virus serodiagnosis. 相似文献
78.
Stiebler R Soares JB Timm BL Silva JR Mury FB Dansa-Petretski M Oliveira MF 《Journal of bioenergetics and biomembranes》2011,43(1):93-99
Blood-feeding organisms digest hemoglobin, releasing large quantities of heme inside their digestive tracts. Free heme is
very toxic, and these organisms have evolved several mechanisms to protect against its deleterious effects. One of these adaptations
is the crystallization of heme into the dark-brown pigment hemozoin (Hz). Here we review the process of Hz formation, focusing
on organisms other than Plasmodium that have contributed to a better understanding of heme crystallization. Hemozoin has been found in several distinct classes
of organisms including protozoa, helminths and insects and Hz formation is the predominant form of heme detoxification. The
available evidence indicates that amphiphilic structures such as phospholipid membranes and lipid droplets accompanied by
specific proteins play a major role in heme crystallization. Because this process is specific to a number of blood-feeding
organisms and absent in their hosts, Hz formation is an attractive target for the development of novel drugs to control illnesses
associated with these hematophagous organisms. 相似文献
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Adriano Nunes-Nesi Alexandra Florian Andrew Howden Kathrin Jahnke Stefan Timm Hermann Bauwe Lee Sweetlove Alisdair R. Fernie 《植物生理与分子生物学学报》2014,(1):248-251
Photorespiration is an essential metabolic process in leaves that facilitates recovery of carbon lost by the oxygenase reaction of Rubisco and avoids the accumulation of the toxic product, 2-phosphoglycolate (2PG) of this reaction (Bauwe et al., 2012). However, there is also evidence to suggest that photorespiration has a more complex role during normal growth than the mere detoxification of 2PG and the recovery of 3-phosphoglycerate (3PGA) (Bauwe et al., 2012). 相似文献