全文获取类型
收费全文 | 5659篇 |
免费 | 543篇 |
国内免费 | 5篇 |
出版年
2023年 | 29篇 |
2022年 | 61篇 |
2021年 | 147篇 |
2020年 | 87篇 |
2019年 | 108篇 |
2018年 | 149篇 |
2017年 | 120篇 |
2016年 | 215篇 |
2015年 | 297篇 |
2014年 | 343篇 |
2013年 | 376篇 |
2012年 | 518篇 |
2011年 | 515篇 |
2010年 | 303篇 |
2009年 | 266篇 |
2008年 | 367篇 |
2007年 | 359篇 |
2006年 | 329篇 |
2005年 | 262篇 |
2004年 | 281篇 |
2003年 | 219篇 |
2002年 | 241篇 |
2001年 | 60篇 |
2000年 | 54篇 |
1999年 | 47篇 |
1998年 | 66篇 |
1997年 | 38篇 |
1996年 | 33篇 |
1995年 | 31篇 |
1994年 | 23篇 |
1993年 | 17篇 |
1992年 | 24篇 |
1991年 | 19篇 |
1990年 | 22篇 |
1989年 | 18篇 |
1988年 | 14篇 |
1987年 | 9篇 |
1986年 | 12篇 |
1985年 | 13篇 |
1984年 | 21篇 |
1982年 | 12篇 |
1981年 | 12篇 |
1980年 | 5篇 |
1979年 | 7篇 |
1978年 | 7篇 |
1977年 | 6篇 |
1975年 | 5篇 |
1974年 | 5篇 |
1971年 | 7篇 |
1967年 | 5篇 |
排序方式: 共有6207条查询结果,搜索用时 508 毫秒
41.
Lectin-binding glycoproteins in seven populations of two burrowing nematode sibling species were probed with five different biotinylated lectins on Western blots, and differences were correlated with nematode ability to parasitize citrus and to overcome citrus rootstock resistance. Banding patterns of molecular weight standards were fit best by an exponential decay function, and a predictive equation was used to estimate molecular weights (r² = 0.999). A band (131 kDa) that labeled with the lectin Concanavalin A (Con A) occurred in extracts from cuticles and egg shells of populations of Radopholus citrophilus that parasitize citrus. Wheat germ agglutin labeled a band (58 kDa) in aqueous homogenates of populations that reproduce in roots of citrus rootstock normally resistant to burrowing nematodes. The two sibling species R. citrophilus and R. similis were distinguished by a high molecular weight Con A-labeled band (608 kDa) from cuticle and egg shells. Probing blots with the lectin Limulus polyphemus agglutinin indicated that each population contained a band (12-16 kDa) specifically inhibited by the addition of 25 mM neuraminic acid, suggesting that glycoproteins with sialic acid moieties are present in burrowing nematodes. 相似文献
42.
The vacuum UV CD bands of repeating DNA sequences are dependent on sequence and conformation 总被引:1,自引:0,他引:1
D M Gray K H Johnson M R Vaughan P A Morris J C Sutherland R L Ratliff 《Biopolymers》1990,29(2):317-323
CD spectra were obtained for eight synthetic double-stranded DNA polymers down to at least 175 nm in the vacuum uv. Three sets of sequence isomers were studied: (a) poly[d(A-C).d(G-T)] and poly[d(A-G).d(C-T)], (b) poly[d(A-C-C).d(G-G-T)] and poly[d(A-C-G).d(C-G-T)], and (c) poly[d(A).d(T)], poly[d(A-T).d(A-T)], poly[d(A-A-T).d(A-T-T)], and poly[d(A-A-T-T).d(A-A-T-T)]. There were significant differences in the CD spectra at short wavelengths among each set of sequence isomers. The (G.C)-containing sequences had the largest vacuum uv bands, which were positive and in the wavelength range of 180-191 nm. There were no large negative bands at longer wavelengths, consistent with the polymers all being in right-handed conformations. Among the set of sequences containing only A.T base pairs, poly[d(A).d(T)] had the largest vacuum uv CD band, which was at 190 nm. This CD band was not present in the spectra of the other (A.T)-rich polymers and was absent from two first-neighbor estimations of the poly[d(A).d(T)] spectrum obtained from the other three sequences. We concluded that the sequence dependence of the vacuum uv spectra of the (A.T)-rich polymers was due in part to the fact that poly[d(A).d(T)] exists in a noncanonical B conformation. 相似文献
43.
Factors affecting population density, group size and territory size of the European badger, Meles meles 总被引:4,自引:0,他引:4
This paper discusses the relationship between the distribution and biomass of the main prey of European badgers, Meles meles and the badgers group size, territory size and population density. The distribution of areas rich in earthworms, Lumbricus spp., is correlated with badger range size, whilst badger group size increases with the biomass of worms per badger territory and badger density increases with overall worm biomass. Regulation of badger density in an area is likely to take place through regulation of group size, in the absence of other factors such as persecution and lack of suitable sett-sites. 相似文献
44.
The genormic organization of the multiple actin DNA sequences in the lower eukaryote Physarum polycephalum was investigated by Mendelian mapping. Actin-homologous restriction endonuclease cleavage fragments detected by DNA blotting showed length polymorphisms when different strains were compared. These length polymorphisms were used as phenotypic markers for actin sequences in the genome. The meiotic assortment of the polymorphic restriction fragments was analysed, revealing four unlinked actin loci. The data for three-of the actin loci, ardB, C and D, are consistent with a single sequence or gene at each locus. The data for the other actin locus. ardA, is consistent with multiple linked actin sequences or genes. 相似文献
45.
Synaptic transmission in cerebral tissue fails very rapidly in the absence of oxygen; the metabolic basis for this is not known. We report here that the transmission failure in the guinea pig hippocampal slice can be delayed threefold by exposing the tissue to extracellular creatine (Cr) for 3 h. The improved survival is associated with an increase of tissue phosphocreatine (PCr) concentration. These data argue that the metabolic basis for synaptic transmission failure is a fall in tissue ATP concentrations. They also indicate a way to protect brain tissue against anoxic damage. 相似文献
46.
The levels of the four deoxyribonucleoside triphosphate pools and the distribution of cells in the various phases of the cell cycle have been examined in Chinese hamster cells as thymidine, present as a regular constituent in the growth medium, was removed in stages. The results indicate that: 1. Duration of the DNA synthetic phase was lengthened when thymidine was removed from the growth medium. 2.Temporally correlated with lengthening of the DNA synthetic phase upon thymidine removal was a 7-fold increase in level of the dCTP pool, reduction in the dGTP pools, and little or no change in dATP pool. 3.Radioactive labeling procedures indicated that expansion of the dCTP pool could be completely accounted for by increased ribonucleotide reductase activity and that the dTTP pool switched from a largely exogenous thymidine source to endogenous dTTP synthesis as the extracellular thymidine concentration was reduced. 4.Deoxyuridine and thymidine were apparently transported by the same system in Chinese hamster cells, while deoxycytidine was transported by a different system. Although deoxycytidine transport was unaffected by thymidine, phosphorylation of intracellular deoxycytidine compounds to the triphosphate level was stimulated by thymidine. Cytidine transport was not significantly affected by thymidine. 相似文献
47.
J Timár 《Acta physiologica Hungarica》1989,74(3-4):259-266
The recovery of MAO-B activity after a single dose of 0.25 mg/kg s.c. (-)deprenyl was measured in rat, by following the time course of the changes in the PEA (phenyl-ethylamine)-induced hyperactivity. One hour after its administration (-)deprenyl enhanced both the PEA-induced locomotion and stereotypy, however, its effect on the latter was more marked. At 24 h and at later time-points only stereotype was enhanced. The results show that a single small dose of (-)deprenyl which selectively blocks MAO-B, causes a long-lasting inhibition of PEA metabolism, and the enzyme activity needs more than one week to restore completely. 相似文献
48.
49.
Emmanuël Sérusiaux Juan Carlos Villarreal A. Tim Wheeler Bernard Goffinet 《Journal of Biogeography》2011,38(6):1138-1151
Aim We reconstructed the phylogeny of the lichen genus Nephroma (Peltigerales) to assess the relationships of species endemic to Macaronesia. We estimated dates of divergences to test the hypothesis that the species arose in Macaronesia (neo‐endemism) versus the oceanic archipelagos serving as refugia for formerly widespread taxa (palaeo‐endemism). Location Cosmopolitan with a special focus on the archipelagos of the Azores, Madeira and the Canary Islands. Methods DNA sequences were obtained from 18 species for three loci and analysed using maximum parsimony, maximum likelihood and Bayesian inferences. Divergence dates were estimated for the internal transcribed spacer (ITS)‐based phylogeny using a relaxed molecular clock. Reconstruction of the ancestral geographical range was conducted using the Bayesian 50% majority rule consensus tree under a parsimony method. Results The backbone phylogenetic tree was fully supported, with Nephroma plumbeum as sister to all other species. Four strongly supported clades were detected: the Nephroma helveticum, the N. bellum, the N. laevigatum and the N. parile clades. The latter two share a common ancestor and each includes a widespread Holarctic species (N. laevigatum and N. parile, respectively) and all species endemic to Macaronesia. The data suggest a neo‐endemic origin of Macaronesian taxa, a recent range expansion from Macaronesia of both widespread species, a range expansion limited to the Mediteranean Basin and south‐western Europe for another taxon, and a long dispersal event that resulted in a speciation event in the western parts of North America. Main conclusions The Macaronesian endemic species belong to two sister clades and originated from a most recent common ancestor (MRCA) shared with one widely distributed taxon, either N. parile or N. laevigatum. Estimates of the mean divergence dates suggest that the endemics originated in the archipelagos after the rise of the volcanic islands, along with the ancestor to the now widespread species, which probably expanded their range beyond Macaronesia via long‐distance dispersal. This study provides the first phylogenetic evidence of Macaronesian neo‐endemism in lichenized fungi and provides support for the hypothesis that oceanic islands may serve as a source for the colonization of continents. However, further data are needed to properly assess the alternative hypothesis, namely colonization from western North America. 相似文献
50.
Tim R. New 《Journal of Insect Conservation》2014,18(1):147-148