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811.
Till Bey Suraj Jamge Sonja Klemme Dorota Natalia Komar Sabine Le Gall Pawel Mikulski 《Epigenetics》2016,11(8):625-634
In January 2016, the first Epigenetic and Chromatin Regulation of Plant Traits conference was held in Strasbourg, France. An all-star lineup of speakers, a packed audience of 130 participants from over 20 countries, and a friendly scientific atmosphere contributed to make this conference a meeting to remember. In this article we summarize some of the new insights into chromatin, epigenetics, and epigenomics research and highlight nascent ideas and emerging concepts in this exciting area of research. 相似文献
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Blood platelets have the capacity to participate in a number of physiological as well as pathological processes within the circulation. In order to evaluate their cellular reactivity a number of platelet function tests have been developed. The mainin vitro function tests are assessment of aggregation and adhesion, secretion, arachidonate metabolism, coagulant activities and the characterization of surface membrane glycoproteins (Day and Rao, 1986). Here we measure alterations of the G-/F-actin equilibrium of platelets. High F-actin values of unstimulated platelets indicate a hyperreactivity of the cell as examined in platelets from diabetics. Determination of the actin filament content in platelets can be considered as a new sensitive function test. 相似文献
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Spengler Laura Jepsen Dirk Zimmermann Till Wichmann Paula 《The International Journal of Life Cycle Assessment》2020,25(5):936-946
The International Journal of Life Cycle Assessment - A basic principle for Type I ecolabels is to consider the whole product life cycle in order to avoid transferring impacts from one life cycle... 相似文献
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Patrick Bongartz Tobias Karmainski Moritz Meyer John Linkhorst Till Tiso Lars M. Blank Matthias Wessling 《Biotechnology and bioengineering》2023,120(5):1269-1287
Bioreactors are the operative backbone, for example, for the production of biopharmaceuticals, biomaterials in tissue engineering, and sustainable substitutes for chemicals. Still, the Achilles' heel of bioreactors nowadays is the aeration which is based on intense stirring and gas sparging, yielding inherent drawbacks such as shear stress, foaming, and sterility concerns. We present the synergistic combination of simulations and experiments toward a membrane stirrer for the efficient bubble-free aeration of bioreactors. A digital twin of the bioreactor with an integrated membrane-module stirrer (MemStir) was developed with computational fluid dynamics (CFD) studies addressing the determination of fluid mixing, shear rates, and local oxygen concentration. Usability of the MemStir is shown in a foam-free recombinant production process of biosurfactants (rhamnolipids) from glucose with different strains of Pseudomonas putida KT2440 in a 3-L vessel and benchmarked against a regular aerated process. The MemStir delivered a maximal oxygen transfer rate (OTRmax) of 175 mmol L−1 h−1 in completely foam-free cultivations. With a high space-time yield (STY) of 118 mgRL L−1 h−1 during a fed-batch fermentation, the effectiveness of the novel MemStir is demonstrated. Simulations show the generic value of the MemStir beyond biosurfactant production, for example, for animal cell cultivation. 相似文献