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Informative genomic microsatellite markers for efficient genotyping applications in sugarcane 总被引:1,自引:0,他引:1
Parida SK Kalia SK Kaul S Dalal V Hemaprabha G Selvi A Pandit A Singh A Gaikwad K Sharma TR Srivastava PS Singh NK Mohapatra T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(2):327-338
Genomic microsatellite markers are capable of revealing high degree of polymorphism. Sugarcane (Saccharum sp.), having a complex polyploid genome requires more number of such informative markers for various applications in genetics
and breeding. With the objective of generating a large set of microsatellite markers designated as Sugarcane Enriched Genomic
MicroSatellite (SEGMS), 6,318 clones from genomic libraries of two hybrid sugarcane cultivars enriched with 18 different microsatellite
repeat-motifs were sequenced to generate 4.16 Mb high-quality sequences. Microsatellites were identified in 1,261 of the 5,742
non-redundant clones that accounted for 22% enrichment of the libraries. Retro-transposon association was observed for 23.1%
of the identified microsatellites. The utility of the microsatellite containing genomic sequences were demonstrated by higher
primer designing potential (90%) and PCR amplification efficiency (87.4%). A total of 1,315 markers including 567 class I
microsatellite markers were designed and placed in the public domain for unrestricted use. The level of polymorphism detected
by these markers among sugarcane species, genera, and varieties was 88.6%, while cross-transferability rate was 93.2% within
Saccharum complex and 25% to cereals. Cloning and sequencing of size variant amplicons revealed that the variation in the number of
repeat-units was the main source of SEGMS fragment length polymorphism. High level of polymorphism and wide range of genetic
diversity (0.16–0.82 with an average of 0.44) assayed with the SEGMS markers suggested their usefulness in various genotyping
applications in sugarcane.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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Sunshine C. Silver Tilak Chandra Egidijus Zilinskas Shourjo Ghose William E. Broderick Joan B. Broderick 《Journal of biological inorganic chemistry》2010,15(6):943-955
Spore photoproduct lyase (SP lyase), a member of the radical S-adenosylmethionine superfamily of enzymes, catalyzes the repair of 5-thyminyl-5,6-dihydrothymine [spore photoproduct (SP)],
a type of UV-induced DNA damage unique to bacterial spores. The anaerobic purification and characterization of Clostridium acetobutylicum SP lyase heterologously expressed in Escherichia coli, and its catalytic activity in repairing stereochemically defined synthetic dinucleotide SPs was investigated. The purified
enzyme contains between 2.3 and 3.1 iron atoms per protein. Electron paramagnetic resonance (EPR) spectroscopy reveals an
isotropic signal centered at g = 1.99, characteristic of a [3Fe–4S]+ cluster accounting for 3–4% of the iron in the sample. Upon reduction, a nearly axial signal (g = 2.03, 1.93 and 1.92) characteristic of a [4Fe–4S]+ cluster is observed that accounts for 34–45% of total iron. Addition of S-adenosylmethionine to the reduced enzyme produces a rhombic signal (g = 2.02, 1.93, 1.82) unique to the S-adenosyl-l-methionine complex while decreasing the overall EPR intensity. This reduced enzyme is shown to rapidly and completely repair
the 5R diastereomer of a synthetic dinucleotide SP with a specific activity of 7.1 ± 0.6 nmol min−1 mg−1, whereas no repair was observed for the 5S diastereomer. 相似文献
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Ishar MP Raj T Agrawal SK Saxena AK Singh L Singh R Bhella SS 《Bioorganic & medicinal chemistry letters》2008,18(17):4809-4812
Baeyer–Villiger oxidation of 5-aryl-7,11,11-trimethyltricyclo[5.4.0.03,6]-undec-1-en-4-ones 4a–h by H2O2 and formic acid in methanol yields mixtures of 3b,7,7-trimethyl-3-phenyl-3,3a,3b,4,5,6,7,8a-octahydro-1H-indeno-[1,2-c]furan-1-ones 8a–h and 3b,7,7-trimethyl-3-phenyl-3,3a,3b,4,5,6,7,8a-octahydro-1H-indeno-[1,2-c]furan-2-ones 9a–h in high yields. The obtained butyrolactones 8a–h display cytotoxic activity against a number of human cancer cells. 相似文献
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