排序方式: 共有273条查询结果,搜索用时 0 毫秒
71.
Sun H Spring S Lapidus A Davenport K Del Rio TG Tice H Nolan M Copeland A Cheng JF Lucas S Tapia R Goodwin L Pitluck S Ivanova N Pagani I Mavromatis K Ovchinnikova G Pati A Chen A Palaniappan K Hauser L Chang YJ Jeffries CD Detter JC Han C Rohde M Brambilla E Göker M Woyke T Bristow J Eisen JA Markowitz V Hugenholtz P Kyrpides NC Klenk HP Land M 《Standards in genomic sciences》2010,3(3):276-284
Desulfarculus baarsii (Widdel 1981) Kuever et al. 2006 is the type and only species of the genus Desulfarculus, which represents the family Desulfarculaceae and the order Desulfarculales. This species is a mesophilic sulfate-reducing bacterium with the capability to oxidize acetate and fatty acids of up to 18 carbon atoms completely to CO(2). The acetyl-CoA/CODH (Wood-Ljungdahl) pathway is used by this species for the complete oxidation of carbon sources and autotrophic growth on formate. The type strain 2st14(T) was isolated from a ditch sediment collected near the University of Konstanz, Germany. This is the first completed genome sequence of a member of the order Desulfarculales. The 3,655,731 bp long single replicon genome with its 3,303 protein-coding and 52 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
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Kiss H Nett M Domin N Martin K Maresca JA Copeland A Lapidus A Lucas S Berry KW Glavina Del Rio T Dalin E Tice H Pitluck S Richardson P Bruce D Goodwin L Han C Detter JC Schmutz J Brettin T Land M Hauser L Kyrpides NC Ivanova N Göker M Woyke T Klenk HP Bryant DA 《Standards in genomic sciences》2011,5(3):356-370
Herpetosiphon aurantiacus Holt and Lewin 1968 is the type species of the genus Herpetosiphon, which in turn is the type genus of the family Herpetosiphonaceae, type family of the order Herpetosiphonales in the phylum Chloroflexi. H. aurantiacus cells are organized in filaments which can rapidly glide. The species is of interest not only because of its rather isolated position in the tree of life, but also because Herpetosiphon ssp. were identified as predators capable of facultative predation by a wolf pack strategy and of degrading the prey organisms by excreted hydrolytic enzymes. The genome of H. aurantiacus strain 114-95(T) is the first completely sequenced genome of a member of the family Herpetosiphonaceae. The 6,346,587 bp long chromosome and the two 339,639 bp and 99,204 bp long plasmids with a total of 5,577 protein-coding and 77 RNA genes was sequenced as part of the DOE Joint Genome Institute Program DOEM 2005. 相似文献
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The scl +18/19 stem cell enhancer is not required for hematopoiesis: identification of a 5' bifunctional hematopoietic-endothelial enhancer bound by Fli-1 and Elf-1 下载免费PDF全文
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Traicoff JL Galperin MM Ramesh A Freebern WJ Haggerty CM Gardner K Knezevic V 《Biochemical and biophysical research communications》2004,323(1):355-360
High throughput technologies are standard methods for analysis of the proteome. Multi-layer multi-well plate dot-blotting system (MLDot) technology is a high-throughput dot blotting system that provides a simple, cost-effective approach for protein expression profiling in multiple samples. In contrast to traditional dot blot, MLDot uses a layered stack of thin, sieve-like membranes in place of a single nitrocellulose membrane. Therefore, up to 10 membranes can be prepared from the samples arrayed in a single 96-well plate. We describe the ability of MLDot to detect the predicted changes in protein expression following multiple mitogen treatment of T-cells. We compare the levels of the phopshorylated forms of CREB, Jun, and Akt in Jurkat T-cells as detected by MLDot to those measured by a gel-based assay. We also describe the ability of MLDot to detect differences in the levels of phosphorylated Akt in Jurkat cells as compared to primary lymphocytes. 相似文献
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Z. D. Knezevic S. S. Siler-Marinkovic L. V. Mojovic 《Applied microbiology and biotechnology》1998,49(3):267-271
Candida rugosa lipase has been used to investigate the hydrolysis of palm oil in a lecithin/isooctane reversed micellar system. The reaction
obeys Michaelis-Menten kinetics for the initial conditions. Kinetic parameters such as maximum rate and Michaelis constant
(K
m) were determined for lipase-catalyzed hydrolysis in n-hexane and isooctane. According to the K
m values, the enzyme affinity towards the substrate was increased in isooctane. The maximum degree of hydrolysis was generally
decreased as the initial substrate concentration was increased. This may suggest that the hydrolysis in lecithin reversed
micelles should be regarded as a one-substrate first-order reversible reaction. It is shown in this study that the proposed
one-substrate first-order kinetic model can serve for the precise prediction of the degree of hydrolysis for a known reaction
time or vice versa, when the initial substrate concentration is less than 0.325 mol/dm3. A disagreement with this model was found when the initial substrate concentration was higher than approximately 0.3 mol/dm3. This may be due to the effects of the products on lipase activity or even to the conversion of the reversed micellar system
to other systems.
Received: 16 May 1997 / Received revision: 22 October 1997 / Accepted: 24 October 1997 相似文献
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Axis formation is a highly regulated process in vertebrate embryos. In mammals, inductive interactions between an extra-embryonic layer, the visceral endoderm, and the embryonic layer before gastrulation are critical both for anterior neural patterning and normal primitive streak formation. The role(s) of the equivalent extra-embryonic endodermal layer in the chick, the hypoblast, is still less clear, and dramatic effects of hypoblast on embryonic gene expression have yet to be demonstrated. We present evidence that two genes later associated with the gastrula organizer (Gnot-1 and Gnot-2) are induced by hypoblast signals in prestreak embryos. The significance of this induction by hypoblast is discussed in terms of possible hypoblast functions and the regulation of axis formation in the early embryo. Several factors known to be expressed in hypoblast, and retinoic acid, synergistically induce Gnot-1 and Gnot-2 expression in blastoderm cell culture. The presence of retinoic acid in prestreak embryos has not yet been directly demonstrated, but exogenous retinoic acid appears to mimic the effects of hypoblast rotation on primitive streak extension, raising the possibility that retinoid signaling plays some role in the pregastrula embryo. 相似文献
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Judith W.J. Bergs Przemek M. Krawczyk Tijana Borovski Rosemarie ten Cate Hans M. Rodermond Jan Stap Jan Paul Medema Jaap Haveman Jeroen Essers Chris van Bree Lukas J.A. Stalpers Roland Kanaar Jacob A. Aten Nicolaas A.P. Franken 《DNA Repair》2013,12(1):38-45
In S and G2 phase mammalian cells DNA double strand breaks (DSBs) can potentially be repaired by homologous recombination (HR) or non-homologous end-joining (NHEJ). Results of several studies suggest that these two mechanistically distinct repair pathways can compete for DNA ends. Because HR and NHEJ differ with respect to error susceptibility, generation of chromosome rearrangements, which are potentially carcinogenic products of DSB repair, may depend on the pathway choice. To investigate this hypothesis, the influence of HR and NHEJ inhibition on the frequencies of chromosome aberrations in G2 phase cells was investigated. SW-1573 and RKO cells were treated with mild (41 °C) hyperthermia in order to disable HR and/or NU7441/cisplatin to inactivate NHEJ and frequencies of chromosomal fragments (resulting from unrepaired DSBs) and translocations (products of erroneous DSB rejoining) were studied using premature chromosome condensation (PCC) combined with fluorescence in situ hybridization (FISH).It is shown here that temporary inhibition of HR by hyperthermia results in increased frequency of ionizing-radiation (IR)-induced chromosomal translocations and that this effect is abrogated by NU7441- or cisplatin-mediated inhibition of NHEJ. The results suggest that in the absence of HR, DSB repair is shifted to the error-prone NHEJ pathway resulting in increased frequencies of chromosomal rearrangements. These results might be of consequence for clinical cancer treatment approaches that aim at inhibition of one or more DSB repair pathways. 相似文献
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