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881.
Maarten Schrama Pieter Heijning Jan P. Bakker Harm J. van Wijnen Matty P. Berg Han Olff 《Oecologia》2013,172(1):231-243
Studies addressing the role of large herbivores on nitrogen cycling in grasslands have suggested that the direction of effects depends on soil fertility. Via selection for high quality plant species and input of dung and urine, large herbivores have been shown to speed up nitrogen cycling in fertile grassland soils while slowing down nitrogen cycling in unfertile soils. However, recent studies show that large herbivores can reduce nitrogen mineralization in some temperate fertile soils, but not in others. To explain this, we hypothesize that large herbivores can reduce nitrogen mineralization in loamy or clay soils through soil compaction, but not in sandy soils. Especially under wet conditions, strong compaction in clay soils can lead to periods of soil anoxia, which reduces decomposition of soil organic matter and, hence, N mineralization. In this study, we use a long-term (37-year) field experiment on a salt marsh to investigate the hypothesis that the effect of large herbivores on nitrogen mineralization depends on soil texture. Our results confirm that the presence of large herbivores decreased nitrogen mineralization rate in a clay soil, but not in a sandy soil. By comparing a hand-mown treatment with a herbivore-grazed treatment, we show that these differences can be attributed to herbivore-induced changes in soil physical properties rather than to above-ground biomass removal. On clay soil, we find that large herbivores increase the soil water-filled porosity, induce more negative soil redox potentials, reduce soil macrofauna abundance, and reduce decomposition activity. On sandy soil, we observe no changes in these variables in response to grazing. We conclude that effects of large herbivores on nitrogen mineralization cannot be understood without taking soil texture, soil moisture, and feedbacks through soil macrofauna into account. 相似文献
882.
Background and Aims
Great attention has been paid to N2O emissions from paddy soils under summer rice-winter wheat double-crop rotation, while less focus was given to the NO emissions. Besides, neither mechanism is completely understood. Therefore, this study aimed at evaluating the relative importance of nitrification and denitrification to N2O and NO emissions from the two soils at different soil moisture contentsMethods
N2O and NO emissions during one winter wheat season were simultaneously measured in situ in two rice-wheat based field plots at two different locations in Jiangsu Province, China. One soil was neutral in pH with silt loam texture (NSL), the other soil alkaline in pH with a clay texture (AC). A 15?N tracer incubation experiment was conducted in the laboratory to evaluate the relative importance of nitrification and denitrification for N2O and NO emissions at soil moisture contents of 40 % water holding capacity (WHC), 65 % WHC and 90 % WHC.Results
Higher N2O emission rates in the AC soil than in the NSL soil were found both in the field and in the laboratory experiments; however, the differences in N2O emissions between AC soil and NSL soil were smaller in the field than in the laboratory. In the latter experiment, nitrification was observed to be the more important source of N2O emissions (>70 %) than denitrification, regardless of the soils and moisture treatments, with the only exception of the AC soil at 90 % WHC, at which the contributions of nitrification and denitrification to N2O emissions were comparable. The ratios of NO/N2O also supported the evidence that the nitrification process was the dominant source of N2O and NO both in situ and in the laboratory. The proportion of nitrified N emitted as N2O (P N2O ) in NSL soil were around 0.02 % in all three moisture treatments, however, P N2O in the AC soil (0.04 % to 0.10 %) tended to decrease with increasing soil moisture content.Conclusions
Our results suggest that N2O emission rates obtained from laboratory incubation experiments are not suitable for the estimation of the true amount of N2O fluxes on a field scale. Besides, the variations of P N2O with soil property and soil moisture content should be taken into account in model simulations of N2O emission from soils. 相似文献883.
Hyo Jae Kang Young Sik Park Chang-Hoon Lee Sang-Min Lee Jae-Joon Yim Chul-Gyu Yoo Young Whan Kim Sung Koo Han Jong-Yil Chai Jinwoo Lee 《The Korean journal of parasitology》2013,51(5):569-572
Dirofilariasis is a rare disease in humans. We report here a case of a 48-year-old male who was diagnosed with pulmonary dirofilariasis in Korea. On chest radiographs, a coin lesion of 1 cm in diameter was shown. Although it looked like a benign inflammatory nodule, malignancy could not be excluded. So, the nodule was resected by video-assisted thoracic surgery. Pathologically, chronic granulomatous inflammation composed of coagulation necrosis with rim of fibrous tissues and granulations was seen. In the center of the necrotic nodules, a degenerating parasitic organism was found. The parasite had prominent internal cuticular ridges and thick cuticle, a well-developed muscle layer, an intestinal tube, and uterine tubules. The parasite was diagnosed as an immature female worm of Dirofilaria immitis. This is the second reported case of human pulmonary dirofilariasis in Korea. 相似文献
884.
Metaeritrichium has been considered monotypic and endemic to China in traditional taxonomic treatments. However, our examinations of specimens and bibliography, combined with field observations, reveal that Metaeritrichium microuloides is conspecific with Actinocarya acaulis. Metaeritrichium is therefore treated as a synonym of Actinocarya. We also clarify the typification of Actinocarya acaulis. 相似文献
885.
Synergistic roles of leaf boron and calcium during the growing season in affecting sugar and starch accumulation in ripening apple fruit 总被引:3,自引:0,他引:3
Yan Qing Lu Hui Ping Liu Yi Wang Xin Zhong Zhang Zhen Hai Han 《Acta Physiologiae Plantarum》2013,35(8):2483-2492
Fruit sugar content is one of the most important flavor quality traits in the fresh market. Minerals, such as boron (B) and calcium (Ca), are associated with fruit sugar and starch accumulation in many plant species. To better understand the roles of B and Ca in affecting sugar and starch accumulation in apples, 2 g L?1 Na2B4O7·10H2O or 10 g L?1 CaCl2 was supplied by foliar spray to 20-year-old ‘Fuji’ (Malus domestica Borkh. cv. Fuji) trees at four developmental stages (fruit set, onset of rapid fruit growth, rapid fruit growth and the end of rapid fruit growth), in 2010–2011. The most effective treatment significantly increasing soluble sugar and starch levels in ripening fruit was the foliar application of 2 g L?1 Na2B4O7·10H2O during rapid fruit growth, and the robustness of the effects was confirmed for two cultivars, ‘Fuji’ and ‘Orin’, at three orchards in 2011. Foliar applications of B during the onset of rapid fruit growth and rapid fruit growth, as well as the foliar application of Ca at fruit set, significantly increased the soluble sugar content in ripening fruit. In addition, the B application was effective in increasing the fruit starch content, but Ca was not. Both B and Ca treatments significantly increased the leaf concentrations of the other element at least transiently. However, B and Ca effects on fruit sugar/starch did not seem to depend on higher leaf B or Ca levels. In conclusion, B and Ca interact in enhancing fruit sugar and starch contents at the fruit ripening stage. 相似文献
886.
Adiantum reniforme var. sinensis (Adiantaceae) is an endangered fern endemic to the Three Gorges region in southwest China. To elucidate possible ecophysiological mechanisms restricting its distribution, effects of the availability of light (28%, 14% and 7% of open field) and soil moisture (60% and 40% of field capacity) on dry matter production and allocation, leaf morphology and water use efficiency (WUE) were examined in A. reniforme var. sinensis and its widespread congener A. capillus‐veneris. Both species had lower root/shoot ratio (R/S) and higher specific leaf area (SLA) when grown at low light. However, A. reniforme var. sinensis showed less plasticity for total leaf area (LA) and leaf area ratio (LAR) than A. capillus‐veneris, and its root mass, total mass and WUE decreased as light availability decreased. Under water stress, all traits of both species except WUE were significantly affected. However, drought stress decreased total mass of A. capillus‐veneris but did not have a significant effect on A. reniforme var. sinensis. Compared with A. capillus‐veneris, A. reniforme var. sinensis had significantly higher R/S but lower values for other analyzed traits. These results suggest that A. reniforme var. sinensis is relatively superior in drought tolerance but inferior at low light, allowing it to persist in habitats with low soil moisture and high light availability but with few coexisting species present. 相似文献
887.
888.
Hyuk Lee Si Jae Park Mee-Jung Han Gyeong Tae Eom Min-Jung Choi Seong Ho Kim Young Hoon Oh Bong Keun Song Seung Hwan Lee 《Biotechnology letters》2013,35(10):1677-1683
Microbial-surface display is the expression of proteins or peptides on the surface of cells by fusing an appropriate protein as an anchoring motif. Here, the outer membrane protein W (OmpW) was selected as a fusion partner for functional expression of Pseudomonas fluorescence SIK W1 lipase (TliA) on the cell-surface of Escherichia coli. Localization of the truncated OmpW-TliA fusion protein on the cell-surface was confirmed by immunoblotting and functional assay of lipase activity. Enantioselective hydrolysis of rac-phenylethyl butanoate by the displayed lipase resulted in optically active (R)-phenyl ethanol with 96 % enantiomeric excess and 44 % of conversion in 5 days. Thus, a small outer membrane protein OmpW, is a useful anchoring motif for displaying an active enzyme of ~50 kDa on the cell-surface and the surface-displayed lipase can be employed as an enantioselective biocatalyst in organic synthesis. 相似文献
889.
Linlin Han Lin Hou Jinlian Song Dongliang Lin Li Wu Yinlin Ge Zhongliang Ma 《Biotechnology letters》2013,35(8):1175-1181
Loss of DBC2 (deleted in breast cancer 2) gene expression is frequent in breast cancer tissues. This can be explained by homozygous deletions or other mutations in a minority of cases but alternative mechanisms need to be investigated. Here, DBC2 expression was significantly suppressed compared with normal breast tissues in breast cancer tissues when analyzed by RT-PCR. Furthermore, DNA methylation on DBC2 was more prevalent in breast tumors than in normal tissues. DBC2 mRNA levels correlated with the degree of DBC2 methylation in breast cancer tissues and in a breast cancer cell line (T47D). Clinico-pathological correlation analysis showed that DBC2 promoter methylation was associated with tumor-node-metastasis stages II and III/IV, lymph node metastasis, p53 mutation, and HER2-positive status. Thus loss of DBC2 expression is caused by abnormal methylation of DBC2 and might have a role in breast cancer development. 相似文献
890.
AbstractHere, we employed DNA-based stable isotope probing (SIP) and molecular biology methods to investigate active ammonia oxidizer communities in suboxic sediments (0 to –2?cm) at the micromolar oxygen level and layers (–2 to –5?cm) at nanomolar oxygen concentrations from meso-eutrophic and light-eutrophic locations in Taihu Lake. The results revealed that ammonia-oxidizing archaea (AOA) were less active in the anoxic layer of meso-eutrophic sites, while ammonia-oxidizing bacteria (AOB) were less active in suboxic sediments of light-eutrophic sites after 8?weeks of incubation. The active AOA in the meso- and light-eutrophic sediments belonged to the Nitrosopumilus, Nitrosotalea, and Nitrososphaera clusters and the Nitrosopumilus and Nitrososphaera clusters, respectively, with Nitrosopumilus cluster as the predominant AOA, which took up a higher ratio in the light-eutrophic and suboxic layers than their counterparts. The advantageous active AOB were numerically predominated by the Nitrosomonas cluster in the suboxic layers, and the Nitrosospira cluster in the anoxic layers, respectively, both of which were distributed in diverse frequencies in different eutrophication statuses. The role and community composition diversities of active ammonia oxidizers in freshwater sediments were attributed to the different eutrophication (including nitrogen and organic carbon content) and oxygen statuses. 相似文献