Analysis of Headspace of Root of Levisticum officinale

The headspace of root of Levistlcum officinale was subjected to adsorb by means of XAD 4. The chemical components of the headspace obtained were analysed by GC/MS and GC/FTIR. 64 compounds have been seperated, of which 20 compounds were identified. The main compounds were monc, terpenoids and sesquiterpenoids, such as β-phellandrens (16.47%), citronellal (12.85%) and ligustilide (20.94%) etc.     

Studies on the Chemical Constituents of Limonium aureum (L.) Hill

Five different crystals have been obtained for the first time from the aerial parts of Limonium aureum (L.)Hill ex kuntze. They were identified as follows: (Ⅰ) homoeriodictyol, (Ⅱ) naringenin, (Ⅲ) eriodictyol (Ⅳ) myricetin-3-O-β-D-glucoside and (Ⅴ) myricetin-3-O-β-D-galactoside.     

Influences of Ce on the Formation of Chlorophyll-Protein Complexes in Chloroplasts of Cucumber Leaves

It has been proven that the Ce content of cucumber (Cucumis sativus L. ) leaves was enhanced with the increase of CeC13 concentration in Hoagland solution. The Chl a/b ratio of cucumber leaves in the control was the same as that in the treated plant, both being 3.67. However, under lower light intensity, the Chl a/b ratio in leaves of the contral was 2.72 whereas that of the treated leaves was 2.86. It showed that only under lower light intensity Ce could decrease the contents of chlorophyll b in leaves. The authors also evidenced that Ce was able to accelerate the formation of chlorophyll-protein complexes of PS Ⅰ and 110 kD polypeptide and decrease the light harvesting complex protein and 27 kD polypeptide.     

Protein Electrophoresis of Single Pollen Grains of Hibiscus rosa-sinensis

Electrophoresis and staining of proteins from the single pollen grains of Hibiscus rosasinensis have been developed by using general ultrathin polyacrylamide gel combined with highly-sensitive silver staining technique. The result revealed that the pollen abortion could occur in different stages of pollen development. The protein patterns varied greatly in different stages of pollen development, even in the different pollen grains in the same anther at the same development stage. Some bands exhibited a disjunction by classical Mendelian ratio 1: 1, suggesting that the gene loci were heterogeneous and the proteins were related to the expression of the genes at the early stage of pollen development.     

Studies on 6-benzyladenine Localization in Callus Cells of Tobacco

The localization of 6-benzyladenine in the callus cell of tobacco was studied by means of autoradiography. Electron microscope autoradiographs showed that the silver grains localized at not only cell membrane system such as plasma membrane, tonoplast, plastid membrane and mitochondrion membrane but also nucleus especially nucleolus. The results of the present study indicated that 6-benzyladenine receptor site associated with both cell membrane system and nucleus.     

The Protein Body in Dedifferentiating Cels of Tobacco Leaf Explants

Light and electron microscopic observations show that a kind of spherical electron-dense body appears in the dediffercntiating mesophyll cells and their subdivided cells in tobacco leaf explants cultured for more than two days. The larger electron-dense bodies (1.0–1.5μm in diameter) present in vacuoles while the smaller ones (0.1–0.8 m in diameter) in cytoplasm. This implies that the bodies first can be formed in cytoplasm and then secreted into vacuoles. Since the bodies can be fixed with glutaradehyde and 3H- leucine can incorporated into them, they may be recognized as protein bodies. The protein bodies usually closly combined with newly formed cytoplasmic masses so we suggest that they probably play some role in cytoplasmic growth of dedifferentiafing ceils.     

中国菊科菜蓟族植物研究(三)

本文是中国菊科菜蓟族植物研究继三。发现了(艹久)苓菊属(Jurinea)、刺头菊属(Cousinia)、蓟属(Cirsium)、及矢车菊属(Centaurea)的一些新种。现将这些新种记载如下。