西北黄土高原半干旱区全膜覆土穴播对土壤水热环境和小麦产量的影响

全膜覆土穴播是西北黄土高原旱作区大面积应用于密植作物栽培的关键增产技术,可显著提高降水利用率和作物生产力,但目前对其增产机制和环境效应缺乏系统研究分析。在2011—2013年以春小麦陇春27号为试验材料,设全膜覆土穴播(FMS)、地膜覆盖穴播(FM)和露地穴播(CK)3个处理,研究半干旱旱作区全膜覆土穴播的土壤水热效应及其对小麦产量的影响。结果表明,小麦苗期FMS在0—25 cm土层的平均地温比CK提高1.4—3.5℃,但孕穗到灌浆期正午地表地温比FM和CK分别降低5.3—6.4℃和3.1—4.3℃。FMS和FM使小麦拔节前0—200 cm土层土壤贮水量分别增加33.1和29.3 mm,且可促进小麦对深层水分(100—200 cm)的利用,FMS成熟期100—200 cm土层土壤贮水量比播前下降44.4—69.6 mm,较CK高8.4—145.5%,但FMS在休闲期补充土壤水分77—127 mm,分别较FM和CK增加4.5%—40.9%和12.8%—109.5%;FMS的休闲效率为30.5%—52.6%,比CK高12.8%—109.5%,比FM高4.5%—40.9%。基于对土壤水热环境和作物耗水的影响,FMS的产量达1750—3180 kg/hm2,水分利用效率为5.5—11.5 kg hm-2mm-1,分别比CK增加40%—220%和27%—239%,而且干旱年份的增加幅度更高。因此,FMS改善了小麦生长前期的土壤水热条件,调节作物不同生育期的耗水强度,显著提高作物水分利用效率和产量,并提高降水休闲效率,使小麦生育期耗散的土壤水分在休闲期得到有效补充。     

Biologically Inspired Scene Context for Object Detection Using a Single Instance

This paper presents a novel object detection method using a single instance from the object category. Our method uses biologically inspired global scene context criteria to check whether every individual location of the image can be naturally replaced by the query instance, which indicates whether there is a similar object at this location. Different from the traditional detection methods that only look at individual locations for the desired objects, our method evaluates the consistency of the entire scene. It is therefore robust to large intra-class variations, occlusions, a minor variety of poses, low-revolution conditions, background clutter etc., and there is no off-line training. The experimental results on four datasets and two video sequences clearly show the superior robustness of the proposed method, suggesting that global scene context is important for visual detection/localization.     

HO‐1 regulates the function of Treg: Association with the immune intolerance in vitiligo

In vitiligo, cutaneous depigmentation is accompanied by increased T cell cytolytic activity targeting melanocytes, indicating that autoimmune tolerance is disrupted. The inhibited amount and function of Tregs have been indicated to be involved in the autoimmune intolerance in vitiligo, however, with the conclusion still controversial and the involved mechanism unknown. In this study, we explored the molecular and cellular alterations accounting for the impaired Treg response in vitiligo. Our results showed that the amount of Tregs was drastically reduced in peripheral blood of active vitiligo patients. Furthermore, the immunoregulatory function of Tregs was attenuated, with lower expression of CTLA4, IL‐10 and TGF‐β. Moreover, the expression of HO‐1, a functional modulator of Tregs, was decreased in vitiligo Tregs, and the concentrations of HO‐1 metabolites, including bilirubin, CoHb and iron, were correspondingly decreased in serum of vitiligo patients. In addition, we treated the Tregs from vitiligo patients with Hemin, an agonist of HO‐1, and found that enhanced HO‐1 expression restored the function of Tregs by up‐regulating IL‐10 expression. Our study demonstrates the essential role of HO‐1 in the impaired Treg response in vitiligo and indicates the potential of HO‐1 as a therapeutic target in vitiligo management.     

Narbonin,a novel 2S protein from Vicia narbonensis L. seeds: cDNA,gene structure and developmentally regulated formation

cDNA and genomic clones encoding narbonin, a 2S globulin from the seed of narbon bean (Vicia narbonensis L.), were obtained using the polymerase chain reaction (PCR) and sequenced. The full-length cDNA as well as genomic clones contain a single open reading frame (ORF) of 873 bp that encodes a protein with 291 amino acids comprising the mature narbonin polypeptide (M _r ca. 33 100) and an initiation methionine. The deduced amino acid sequence lacks a transient N-terminal signal peptide. The genomic clones do not contain any intron. No homology was found to nucleic acid and protein sequences so far registered in sequence data libraries. The biosynthesis of narbonin during embryogenesis is developmentally-regulated and its pattern of synthesis closely resembles that of typical seed storage globulins. However, during seed germination narbonin was degraded very slowly, indicating that it may have other function than storage protein. Southern analysis suggests the existence of a small narbonin gene family. Narbonin genes were also found in four different species of the genus Vicia as well as in other legumes such as Canavalia ensiformis and Glycine max. In Escherichia coli a recombinant narbonin was produced which yielded crystals like those prepared from narbonin purified from seeds.     

脊髓提取液对培养脊髓神经细胞中GABA能及DYN能神经元突起生长的影响

本文以体外培养的小鼠脊髓神经元为模型研究了人胚脊髓提取液对Ｅ１２－１５小鼠脊髓中ＧＡＢＡ能神经元和ＤＮＹ能神经元的突起生长的营养作用,结果发现人胚脊髓提取液在蛋白浓度为２５０μｇ／ｍｌ时对ＧＡＢＡ能神经元的突起生长无营养作用,但对ＤＮＹ能神经元的突起生长有显著的促进作用。提示了人胚脊髓提取液中有促进神经元突起生长的营养物质,且对特定胎龄的不同的神经元有不同的作用。     

p21基因的克隆及其对肺癌细胞生长的抑制

用反转录ＰＣＲ从正常人胚胎肺细胞中获得了ｐ２１基因ｃＤＮＡ,将其插入真核表达载体ｐＭＳＣＶｎｅｏ,构建成重组质粒,ｐＭＳ２１,并将其转染至肺癌细胞株Ａ５４９．通过集落形成观察到ｐ２１对肺癌细胞具有明显的抑制作用,经ＲＮＡ狭缝杂交、Ｗｅｓｔ－ｅｒｎｂｌｏｔ分析和免疫细胞化学实验证实这是ｐ２１表达的结果．荷瘤裸鼠实验也进一步证实了ｐ２１对肺癌细胞具有明显的抑制作用．为ｐ２１的深入研究提供了基础．