人早孕子宫蜕膜催乳素分泌的调节

子宫内膜蜕膜化对胚泡植入与妊娠维持是非常重要的。为探讨蜕膜化维持的调节机制 ,本文研究了妊娠早期人子宫蜕膜细胞催乳素 (PRL)分泌的调节。结果表明 :(1)孕酮显著地刺激PRL的分泌。 (2 )雌激素的作用与其浓度有关 ,生理浓度的雌激素对PRL分泌无明显影响 ,而高水平的雌激素抑制孕酮的刺激作用。合适的雌孕激素比例对蜕膜化的维持是必要的。 (3)RU486明显地抑制PRL的分泌 ,故认为孕酮的作用至少是部分通过受体介导的机制。 (4 )高浓度的cAMP (≥ 10 -5mol/L)显著增加PRL的分泌 ,cAMP信号系统可能在蜕膜化反应中发挥重要作用。     

Design of Infrared Plasma Absorber with High Refractive Index Sensitivity

Three layers of periodic artificial metamaterial sensing structure (including the upper metal particles, intermediate dielectric layer, and the lower reflective layer) with ultra-narrow band absorption were designed. The resonance characteristics and sensing properties were analyzed by the finite difference time domain (FDTD) method. The effect of localized surface plasmon resonance (LSPR) was obviously observed at the resonance wavelength of 911 nm, and it achieves nearly perfect absorption of exceeding 98% with a full width at half maximum (FWHM) of 3.5 nm. In addition, a wavelength sensitivity of 542 nm/RIU with a figure of merit (FOM) of 155 was obtained in the refractive index (RI) range from 1.00 to 1.35, which has a wide range of applications. The results show that the proposed structure has high absorption and RI sensitivity, which is suitable for bioengineering and medical detection.

     

妊娠晚期阴道B族链球菌的感染对肠道菌群和妊娠结局的影响

目的探究妊娠晚期阴道B族链球菌(group B Streptococcus,GBS)的感染对肠道菌群和妊娠结局的影响。方法选取2018年3月至2019年11月大连市中心医院孕检并分娩的妊娠妇女744人为对象,调查并统计B族链球菌的感染率;筛选有和没有B族链球菌感染妊娠妇女各47人,调查不良妊娠结局的发生率;选取信息匹配的妊娠晚期阴道B族链球菌感染和未感染的妊娠妇女,采集粪便样本,提取菌群DNA,用16S rDNA方法分析菌群变化。结果744名妊娠妇女中B族链球菌检出49例,感染率为6.59%;B族链球菌感染组总的不良妊娠发生比例为76.6%,正常组发生比例为27.7%(χ^2=5.491,P<0.05)。B族链球菌感染组妊娠妇女胎膜早破(χ^2=16.177,P<0.01)、难产(χ^2=21.134,P<0.01)和羊水异常(χ^2=22.989,P<0.05)的发生率与未感染组比较显著增高。B族链球菌感染组妊娠妇女肠道菌群发生显著变化。结论妊娠晚期阴道B族链球菌的感染可能引起肠道菌群紊乱,增加不良妊娠结局。     

Study of the physiological mechanisms of two species of <Emphasis Type="Italic">Spiraea</Emphasis> during adaptation to drought treatment

The photosynthetic rate, light saturation point, light compensation point, changes in the MDA and SOD activities, and protein expression of two different drought-resistance species, Spiraea fritschiana and Spiraea trichocarpa, were assessed in this study. Furthermore, the drought-resistant physiological mechanisms of both species were analyzed at the protein level. The photosynthetic capacities of two Spiraea species decreased under drought stress, and the light saturation point and light compensation point decreased. However, their capacities to use weak light increased. Spiraea fritschiana, which demonstrated a stronger drought resistance, showed a better ability to adapt to weak light than S. trichocarpa. The content of MDA in S. fritschiana was notably lower than that in S. trichocarpa, indicating that the concentration of the membrane peroxidation products of S. fritschiana was less than those of S. trichocarpa. Compared with S. trichocarpa, S. fritschiana’s SOD activity was higher, and its ability to remove ROS was also better. Sixty-six proteins were identified with significantly different expression behavior and included regulatory, redox homeostasis, metabolism and energy, and cytoskeleton proteins. The results showed that the photosynthesis of S. trichocarpa was significantly affected by the drought stress. Enzymes in photosynthesis changed significantly; the expression of the RuBisCo large subunit decreased; and RuBisCo carboxylase, the chlorophyll a–b binding protein, ATP synthase, OEC 33 kD photosystem II protein and 23 kD OEC protein greatly increased. In addition, four antioxidant enzymes greatly increased, GroES chaperonin decreased, and eIF5A significantly increased under light stress. When S. fritschiana Schneid encountered serious drought stress, in addition to those enzymes that changed significantly under light drought stress in S. trichocarpa Nakai, NAD(P)H-quinone oxidoreductase and eIF5A were up-regulated. Specifically, three heat-shock proteins were induced. The expression of the enzymes of the two Spiraea that were related to photosynthesis, oxidation–reduction and regulation were all affected, but their species and expression patterns were different. In S. trichocarpa Nakai and S. fritschiana Schneid, there were significant changes in the proteins related to energy metabolism and the proteins related to energy transport, respectively. Thus, we considered that, in the case of protein involvement, the differences in the metabolic pathways and adjustment levels might contribute to S. trichocarpa having a weaker drought tolerance than S. fritschiana.     

翅碱蓬对盐沼沉积物微生物生物量及β-氨氧化细菌群落的影响——以双台河口为例

为了解翅碱蓬植被对盐沼沉积物微生物的影响,于2013年7月、8月、9月和11月对双台河口裸滩和翅碱蓬植被沉积物(10—15 cm)微生物生物量碳(MBC)、微生物生物量氮(MBN)、16S rRNA基因丰度、潜在硝化速率、β-氨氧化细菌(β-AOB)丰度及群落进行了调查。结果表明,不同采样日期裸滩沉积物MBC、翅碱蓬沉积物β-AOB amo A丰度和两种生境潜在硝化速率没有显著差异;而翅碱蓬沉积物MBC、裸滩沉积物β-AOB amo A丰度、两种生境MBN和16S rRNA基因丰度呈现时间波动。当所有采样日期的数据结合分析时,翅碱蓬植被显著影响沉积物MBC、MBN、细菌16S rRNA基因丰度、潜在硝化速率和β-AOB amo A丰度。从裸滩和翅碱蓬沉积物获得的β-AOB序列属于Nitrosospira和Nitrosomonas,翅碱蓬植被对β-AOB群落结构和多样性均有一定的影响。研究结果有助于了解翅碱蓬湿地中微生物的作用,为盐沼生境的生态修复技术提供参考。     

多头绒泡菌核仁及其骨架中原肌球蛋白的免疫细胞化学鉴定

分离多头绒泡菌(physarum polycephalum)细胞的核仁,先用Dnase I消化,去除核仁内的DNA;然后用025mol/L (NH4)2ＳＯ４和2mol/L NaCl相继抽提去掉大部分蛋白质,制备成核仁骨架。SDSPAGE分析结果表明,核仁骨架中含有约20种多肽,其中包括37kD左右与原肌球蛋白分子量相当的多肽。以兔抗原肌球蛋白抗体为一抗,FITC标记的羊抗兔IgG抗体为二抗的间接免疫荧光检测结果表明,核仁和核仁骨架样品都能发出明亮的荧光,而对照样品未见明亮的荧光。间接免疫斑点印迹检测结果进一步证明,在核仁骨架的蛋白质成分中存在原肌球蛋白。胶体金免疫电镜检测结果显示,标记原肌球蛋白抗体的标本上有较多的金颗粒,而对照组标本上只有极少的金颗粒。金颗粒在核仁中主要呈散在分布。     

甘露糖结合凝集素对小鼠脾脏CD11c+髓样树突状细胞表型和

目的 探讨甘露聚糖结合凝集素(Mannan-binding lectin,MBL)对CD11c+髓样树突状细胞(CD11c+mDC)表型和功能的影响.方法 应用磁珠分选技术获得BALB/c小鼠脾脏CD1 1c+ mDC和CD4+T淋巴细胞.在CD11c+mDC中加入不同浓度的MBL(2.5～20 μg/mL)刺激,以不加MBL的细胞作为对照,应用ELISA法检测细胞培养上清液中的IL-12水平,流式细胞仪检测细胞表面分子CD40、CD80、CD86及HLA-DR的表达.用MTT法测定CD11c+mDC刺激CD4+T淋巴细胞的增殖能力.ELISA法检测细胞培养液中IL-4和IFN-γ水平.结果 MBL显著增强CD11c+mDC表面分子CD40、CD80、CD86及HLA-DR的表达和IL-12的分泌,促进CD4+T淋巴细胞的增殖和抗原递呈能力,诱导CD4+T向TH1反应分化.结论 MBL能够有效刺激CD11c+mDC的活化,诱导CD4+T淋巴细胞向TH1反应分化.