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991.
Isoenzyme patterns of peroxidase, catalase, glucose-6-phosephate, glutamate and isocitrate dehydrogenases, esterase, amylase and IAA oxidase in the embryos, endosperms, roots and shoots of wheat seedlings (Triticum aestivum L. var. Nung-da 139) were determined by horizontal starch gel electrophoresis and polyacrylamide gel disc electrophoresis respectively. The number of isoenzymes of peroxidasc and amylase was increased with the concomitant increase of days during germination. The isoenzyme bands of esterase, glutamate, glucose-6-phosphate and isocitrate dehydrogenases in the embryos were more in the begining of germination. The activities of pero- xidase, IAA oxidase and glutamate dehydrogenase in roots were higher than those in shoots. On the contrary, the activities of catalase and glucose-6-phosphate dehydrogenase in shoots were higher than those in roots. However the activity of esterasc was slight higher in shoots. There was no difference in the activity of isocitrate dehydrogenase between roots and shoots. The morphological difference of shoot and root is evidently related to isoenzyme patterns. This investigation indicates that different metabolic characters are existed in shoot and root during differentiation. 相似文献
992.
993.
Associations of the PTEN − 9C>G polymorphism with insulin sensitivity and central obesity in Chinese
Qiu Yang Hongyi Cao Shugui Xie Yuzhen Tong Qibo Zhu Fang Zhang Qingguo Lü Yan Yang Daigang Li Mei Chen Changyong Yu Wei Jin Yuquan Yuan Nanwei Tong 《Gene》2013
Background
Phosphatase and tensin homolog on chromosome 10 gene (PTEN) is known as a tumor-suppressor gene. Previous studies demonstrated that PTEN dysfunction affects the function of insulin. However, investigations of PTEN single nucleotide polymorphisms (SNPs) and IR-related disease associations are limited. The aim of the present study was to investigate whether its polymorphism could be involved in the risk of metabolic syndrome (MetS).Methods
The genotype frequency of PTEN − 9C>G polymorphism was determined by using a Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) method in 530 subjects with MetS and 202 healthy control subjects of the Han Ethnic Chinese population in a case–control analysis.Results
The PTEN − 9C>G polymorphism was not associated with MetS or its hyperglycemia, hypertension and hypertriglyceridemia components. In the control individuals aged < 60 years or ≥ 60 years, the CG genotype individuals had lower insulin sensitivity than CC individuals (P < 0.05). In the < 60-year-old MetS group and normal glucose tolerance (NGT) subgroup, the CG individuals had lower insulin sensitivity and higher waist circumference (WC) and waist-height-ratio (WHtR) than CC individuals (P < 0.05). Multiple linear regression analysis showed that the PTEN polymorphism (P = 0.001) contributed independently to 4.2% (adjusted R2) of insulin sensitivity variance (estimated by Matsuda ISI), while age (P = 0.004), gender (P = 0.000) and the PTEN polymorphism (P = 0.032) contributed independently to 5.6% (adjusted R2) of WHtR variance.Conclusions
The CG genotype of PTEN − 9C>G polymorphism was not associated with MetS and some of its components as well. However, it may not only decrease insulin sensitivity in the healthy control and MetS in pre-elderly or NGT subjects, but may also increase the risk of central obesity among these MetS individuals. 相似文献994.
995.
Ring ATPases perform a variety of tasks in the cell. Their function involves complex communication and coordination among the often identical subunits. Translocases in this group are of particular interest as they involve both chemical and mechanical actions in their operation. We study the DNA packaging motor of bacteriophage φ29, and using single-molecule optical tweezers and single-particle cryo-electron microscopy, have discovered a novel translocation mechanism for a molecular motor. 相似文献
996.
Congenital heart defects (CHDs) are the most common birth defects; genes involved in homocysteine/folate metabolism may play important roles in CHDs. Methionine synthase reductase (MTRR) is one of the key regulatory enzymes involved in the metabolic pathway of homocysteine. We investigated whether two polymorphisms (A66G and C524T) of the MTRR gene are associated with CHDs. A total of 599 children with CHDs and 672 healthy children were included; the polymorphisms were detected by PCR and RFLP analysis. Significant differences in the distributions of A66G and C524T alleles were observed between CHD cases and controls, and slightly increased risks of CHD were associated with 66GG and 524CT genotypes (odds ratios = 1.545 and 1.419, respectively). The genotype frequencies of 524CT in the VSD subgroup, 66GG and 524CT in the PDA subgroup were significantly different from those of controls. In addition, the combined 66AA/524CT, 66AG/524CT and 66GG/524CT in CHDs had odds ratios = 1.589, 1.422 and 1.934, respectively. Increased risks were also observed in 66AA/524CT and 66GG/524CT for ASD, 66AG/524CT for VSD, as well as 66GG/524CT for PDA. In conclusion, MTRR A66G and C524T polymorphisms are associated with increased risk of CHDs. 相似文献
997.
Xuan Zhang Jie Zhang Linjiang Tong Yu Luo Mingbo Su Yi Zang Jia Li Wei Lu Yi Chen 《Bioorganic & medicinal chemistry》2013,21(11):3240-3244
A novel class of colchicine-SAHA hybrids were designed and synthesised based on the synergistic antitumor effect of tubulin inhibitors and histone deacetylases (HDAC) inhibitors. To the best of our knowledge, this is the first design of molecules that are dual inhibitors of tubulin and HDAC. Biological evaluations of these compounds included the inhibitory activity of HDAC, in vitro cell cycle analysis in BEL-7402 cells as well as cytotoxicity in five cancer cell lines. 相似文献
998.
从安徽亳州采集表现明显CMV症状的烟草样本,取ELISA检测阳性反应最强的样本提取总RNA。设计特异性引物扩增CMV RNA2部分片段,克隆并测序。其中包含的2b基因全长336个核苷酸,编码111个氨基酸。将来源于安徽烟草的CMV 2b基因与其它CMV 2b基因进行序列比对,结果表明,来源于安徽烟草的CMV 2b基因与来源于韩国的2b基因AF033667的核苷酸序列相似性最高,达98.8%。构建2b基因系统关系树,也表明来源于安徽烟草的CMV 2b基因与AF033667亲缘关系最近。以安徽CMV 2b为模板,分别扩增大小约为300 bp的正向片段CMV 2b(r)和反向片段CMV 2b(i)。先后将反向片段CMV 2b(i)和正向片段CMV 2b(r)分别插入载体pSK-In所含intron两侧,获得重组质粒pSK-2b(r)-In-2b(i)。再将含intron的正反向片段插入pBIN438,最终得到含CMV 2b部分序列反向重复的植物表达载体pBIN-2b(r)-In-2b(i)。 相似文献
999.
David T. Bolick Tufeng Chen Luís Antonio O. Alves Yixin Tong Di Wu Linwood T. Joyner II Reinaldo B. Oriá Richard L. Guerrant Zheng Fu 《PloS one》2014,9(9)
Nutritional deficiency and stress can severely impair intestinal architecture, integrity and host immune defense, leading to increased susceptibility to infection and cancer. Although the intestine has an inherent capability to adapt to environmental stress, the molecular mechanisms by which the intestine senses and responds to malnutrition are not completely understood. We hereby report that intestinal cell kinase (ICK), a highly conserved serine/threonine protein kinase, is a novel component of the adaptive cell signaling responses to protein malnutrition in murine small intestine. Using an experimental mouse model, we demonstrated that intestinal ICK protein level was markedly and transiently elevated upon protein deprivation, concomitant with activation of prominent pro-proliferation and pro-survival pathways of Wnt/β-catenin, mammalian target of rapamycin (mTOR), mitogen-activated protein kinase (MAPK), and protein kinase B (PKB/Akt) as well as increased expression of intestinal stem cell markers. Using the human ileocecal epithelial cell line HCT-8 as an invitro model, we further demonstrated that serum starvation was able to induce up-regulation of ICK protein in intestinal epithelial cells in a reversible manner, and that serum albumin partially contributed to this effect. Knockdown of ICK expression in HCT-8 cells significantly impaired cell proliferation and down-regulated active β-catenin signal. Furthermore, reduced ICK expression in HCT-8 cells induced apoptosis through a caspase-dependent mechanism. Taken together, our findings suggest that increased ICK expression/activity in response to protein deprivation likely provides a novel protective mechanism to limit apoptosis and support compensatory mucosal growth under nutritional stress. 相似文献
1000.
Gadd45a expression induces Bim dissociation from the cytoskeleton and translocation to mitochondria 总被引:9,自引:0,他引:9 下载免费PDF全文
Tong T Ji J Jin S Li X Fan W Song Y Wang M Liu Z Wu M Zhan Q 《Molecular and cellular biology》2005,25(11):4488-4500
Gadd45a, a p53- and BRCA1-regulated stress protein, has been implicated in the maintenance of genomic fidelity, probably through its roles in the control of cell cycle checkpoint and apoptosis. However, the mechanism(s) by which Gadd45a is involved in the induction of apoptosis remains unclear. We show here that inducible expression of Gadd45a protein causes dissociation of Bim, a Bcl2 family member, from microtubule-associated components and translocation to mitochondria. The Bim accumulation in mitochondria enhances interaction of Bim with Bcl-2, relieves Bax from Bcl-2-bound complexes, and subsequently results in release of cytochrome c into the cytoplasm. Suppression of endogenous Bim greatly inhibits Gadd45a induction of apoptosis. Interestingly, Gadd45a interacts with elongation factor 1alpha (EF-1alpha), a microtubule-severing protein that plays an important role in maintaining cytoskeletal stability, and inhibits EF-1alpha-mediated microtubule bundling, indicating that the interaction of Gadd45a with EF-1alpha disrupts cytoskeletal stability. A mutant form of Gadd45a harboring a deletion of EF-1alpha-binding domain fails to inhibit microtubule stability and to induce Bim translocation to mitochondria. Furthermore, coexpression of EF-1alpha antagonizes Gadd45a's property of suppressing cell growth and inducing apoptosis. These findings identify a novel link that connects stress protein Gadd45a to the apoptotic machinery and address the importance of cytoskeletal stability in apoptotic response to DNA damage. 相似文献