Cancer stem cells (CSCs) are a source of tumour recurrence in patients with nasopharyngeal carcinoma (NPC); however, the function of microRNA‐124 (miR‐124) in NPC CSCs has not been clearly defined. In this study, we investigated the role of miR‐124 in NPC CSCs. qRT‐PCR was performed to measure miR‐124 expression in NPC tissues and cell lines and the effects of miR‐124 on stem‐like properties and radiosensitivity of NPC cells measured. Luciferase reporter assays and rescue experiments were used to investigate the interaction of miR‐124 with the 3′UTR of junctional adhesion molecule A (JAMA). Finally, we examined the effects of miR‐124 in an animal model and clinical samples. Down‐regulation of miR‐124 was detected in cancer tissues and was inversely associated with tumour stage and lymph node metastasis. Overexpression of miR‐124 inhibited stemness properties and enhanced radiosensitivity of NPC cells in vitro and in vivo via targeting JAMA. Up‐regulation of miR‐124 was correlated with superior overall survival of patients with NPC. Our study demonstrates that miR‐124 can inhibit stem‐like properties and enhance radiosensitivity by directly targeting JAMA in NPC. These findings provide novel insights into the molecular mechanisms underlying therapy failure in NPC. 相似文献
Watermelon(Citrullus lanatus) as non-climacteric fruit is domesticated from the ancestors with inedible fruits. We previously revealed that the abscisic acid(ABA) signaling pathway gene ClSnRK2.3 might infuence watermelon fruit ripening. However,the molecular mechanisms are unclear. Here,we found that the selective variation of ClSnRK2.3 resulted in lower promoter activity and gene expression level in cultivated watermelons than ancestors, which indicated ClSnRK2.3 might be a negative regulator ... 相似文献
A series of perfluorobutane sulfonate (PFBS)-related substances are produced as the alternatives to corresponding perfluorooctane sulfonate (PFOS). However, there is little reliable information about PFBS available for the whole ecotoxicology compartments. At present, its ecotoxicity of perfluorobutane sulfonate potassium (PFBSK) was selected as a typical PFBS chemical to investigate and evaluate its aquatic, terrestrial, microorganism and sediment toxicity according to the Organization for Economic Cooperation and Development (OECD) guideline under the framework of the REACH regulation. Meanwhile, the ecotoxicology hazard and risk for PFBSK and PFOS were compared comprehensively.
PFBSK did not show acute and chronic aquatic, microorganisms and terrestrial animal toxicity, while exhibited some terrestrial plants toxicity. The ecotoxicology of PFBSK decreased sharply except for terrestrial plants compared with PFOS. Especially, for acute and chronic aquatic ecotoxicity, PFBSK is lower than 100 times those of PFOS.
Although the similar terrestrial plants toxicity level was observed for PFBSK and PFOS, the lower risk of PFBSK was deduced for the reason that there was far less chances to be exposed and retained in the soil and sediment for its high water solubility and low adsorption.
In conclusion, PFBSK showed lower ecotoxicity hazard and risk than those of PFOS. PFBSK could be an environmental friendly alternative to PFOS. 相似文献