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Mariele Porto Carneiro Le?o Patricia Vieira Tiago Fernando Dini Andreote Welington Luiz de Araújo Neiva Tinti de Oliveira 《Genetics and molecular biology》2015,38(1):86-92
The entomopathogenic fungi of the genus Metarhizium have several
subtilisin-like proteases that are involved in pathogenesis and these have been used
to investigate genes that are differentially expressed in response to different
growth conditions. The identification and characterization of these proteases can
provide insight into how the fungus is capable of infecting a wide variety of insects
and adapt to different substrates. In addition, the pr1A gene has
been used for the genetic improvement of strains used in pest control. In this study
we used quantitative RT-PCR to assess the relative expression levels of the
pr1A gene in M. anisopliae and M.
acridum during growth in different culture conditions and during
infection of the sugar cane borer, Diatraea saccharalis Fabricius.
We also carried out a pathogenicity test to assess the virulence of both species
against D. saccharalis and correlated the results with the pattern
of pr1A gene expression. This analysis revealed that, in both
species, the pr1A gene was differentially expressed under the growth
conditions studied and during the pathogenic process. M. anisopliae
showed higher expression of pr1A in all conditions examined, when
compared to M. acridum. Furthermore, M. anisopliae
showed a greater potential to control D. saccharalis. Taken
together, our results suggest that these species have developed different strategies
to adapt to different growing conditions. 相似文献
84.
Animals that select and defend suitable habitats against conspecifics may be favored by maximizing prey encounter rate, gaining protection, or securing matings. However, the identification of habitat selection and territoriality may be hindered in observational studies in sedentary species with low-density populations, such as the whip spider Heterophrynus longicornis. To circumvent such difficulties, we adopted an experimental field approach to evaluate if H. longicornis selects and defends habitat in Central Amazon. To evaluate whether individuals perform habitat selection, we monitored the permanence of 29 experimentally released individuals in buttressed trees with a wide variation in diameter at breast height (DBH), with and without burrows at their bases. To evaluate whether individuals are territorial, we experimentally removed 21 individuals from their previously occupied trees and monitored the recolonization of these trees. If H. longicornis were territorial, we predicted that the recolonizers would be smaller than the removed individuals. We found 12 individuals in the trees where they were released on subsequent days, none of them in trees without burrows. The individual permanence was related to the presence of burrows, and not to DBH. There was recolonization by smaller males and females on ten trees from which the amblypygids were removed. In combination, the permanence of individuals in trees with burrows, the rapid recolonization of experimentally vacated trees by smaller individuals, and the preponderance of one individual per tree, suggest that both male and female H. longicornis perform habitat selection and also that after selecting a site, they defend it against conspecifics. 相似文献
85.
Pauline M. Leite Rodrigo S. Gomes Amanda B. Figueiredo Tiago D. Serafim Wagner L. Tafuri Carolina C. de Souza Sandra A. L. Moura Juliana L. R. Fietto Maria N. Melo Fátima Ribeiro-Dias Milton A. P. Oliveira Ana Rabello Luís C. C. Afonso 《PLoS neglected tropical diseases》2012,6(10)
Background
Leishmania (Viannia) braziliensis has been associated with a broad range of clinical manifestations ranging from a simple cutaneous ulcer to destructive mucosal lesions. Factors leading to this diversity of clinical presentations are not clear, but parasite factors have lately been recognized as important in determining disease progression. Given the fact that the activity of ecto-nucleotidases correlates with parasitism and the development of infection, we evaluated the activity of these enzymes in promastigotes from 23 L. braziliensis isolates as a possible parasite-related factor that could influence the clinical outcome of the disease.Methodology/Principal Findings
Our results show that the isolates differ in their ability to hydrolyze adenine nucleotides. Furthermore, we observed a positive correlation between the time for peak of lesion development in C57BL/6J mice and enzymatic activity and clinical manifestation of the isolate. In addition, we found that L. (V.) braziliensis isolates obtained from mucosal lesions hydrolyze higher amounts of adenine nucleotides than isolates obtained from skin lesions. One isolate with high (PPS6m) and another with low (SSF) ecto-nucleotidase activity were chosen for further studies. Mice inoculated with PPS6m show delayed lesion development and present larger parasite loads than animals inoculated with the SSF isolate. In addition, PPS6m modulates the host immune response by inhibiting dendritic cell activation and NO production by activated J774 macrophages. Finally, we observed that the amastigote forms from PPS6m and SSF isolates present low enzymatic activity that does not interfere with NO production and parasite survival in macrophages.Conclusions/Significance
Our data suggest that ecto-nucleotidases present on the promastigote forms of the parasite may interfere with the establishment of the immune response with consequent impaired ability to control parasite dissemination and this may be an important factor in determining the clinical outcome of leishmaniasis. 相似文献86.
Reduced genetic diversity and increased reproductive isolation follow population‐level loss of larval dispersal in a marine gastropod 下载免费PDF全文
Ryan A. Ellingson Patrick J. Krug 《Evolution; international journal of organic evolution》2016,70(1):18-37
Population‐level consequences of dispersal ability remain poorly understood, especially for marine animals in which dispersal is typically considered a species‐level trait governed by oceanographic transport of microscopic larvae. Transitions from dispersive (planktotrophic) to nondispersive, aplanktonic larvae are predicted to reduce connectivity, genetic diversity within populations, and the spatial scale at which reproductive isolation evolves. However, larval dimorphism within a species is rare, precluding population‐level tests. We show the sea slug Costasiella ocellifera expresses both larval morphs in Florida and the Caribbean, regions with divergent mitochondrial lineages. Planktotrophy predominated at 11 sites, 10 of which formed a highly connected and genetically diverse Caribbean metapopulation. Four populations expressed mainly aplanktonic development and had markedly reduced connectivity, and lower genetic diversity at one mitochondrial and six nuclear loci. Aplanktonic dams showed partial postzygotic isolation in most interpopulation crosses, regardless of genetic or geographic distance to the sire's source, suggesting that outbreeding depression affects fragmented populations. Dams from genetically isolated and neighboring populations also exhibited premating isolation, consistent with reinforcement contingent on historical interaction. By increasing self‐recruitment and genetic drift, the loss of dispersal may thus initiate a feedback loop resulting in the evolution of reproductive isolation over small spatial scales in the sea. 相似文献
87.
Naiara?Guimar?es?Sales Tiago?Casarim?Pessali Francisco?Ricardo?Andrade Neto Daniel?Cardoso?CarvalhoEmail author 《Biological invasions》2018,20(3):555-566
Invasive species are one of the greatest threats to biodiversity, due to competition, predation, pathogen spread, and hybridization. The latter may remain undetected and impair the survival of species, due to genetic admixture and hybrid swarming (i.e., interbreeding between hybrid individuals and backcrossing with parental species). The impact of invasive species remains poorly studied in the Neotropical ichthyofauna, particularly when considering the potential for hybridization between native and introduced species. Due to fisheries importance and its commercial value, species of the Prochilodus genus have been introduced to other catchments in Brazil. Here, we evaluate the introduction of non-native Prochilodus species and the potential effect of hybridization with the native migratory fish P. hartii. To evaluate possible introgression of Prochilodus spp. to P. hartii in the Jequitinhonha river basin (JRB), we employed a morphogenetic approach, analysing 219 specimens sampled from a broad extent of the river basin. Morphological analyses using meristic characters were incongruent with molecular identification by DNA barcoding (COI) in 22.83% of the analysed specimens. Haplotypes from three non-native species (P. argenteus, P. costatus, and P. lineatus) were recovered from specimens morphologically identified as P. hartii. Hybridization between P. hartii and introduced species was confirmed using co-dominant nuclear microsatellite markers. We observed a pronounced introgression pattern in this Neotropical basin, and paradoxically, despite being one of the most abundant migratory species native to the JRB, due to ongoing levels of introgression, P. hartii’s genetic integrity and conservation might be affected. 相似文献
88.
Relationship between lifestyle behaviors and obesity in children ages 9–11: Results from a 12‐country study 下载免费PDF全文
Peter T. Katzmarzyk Tiago V. Barreira Stephanie T. Broyles Catherine M. Champagne Jean‐Philippe Chaput Mikael Fogelholm Gang Hu William D. Johnson Rebecca Kuriyan Anura Kurpad Estelle V. Lambert Carol Maher Jose Maia Victor Matsudo Timothy Olds Vincent Onywera Olga L. Sarmiento Martyn Standage Mark S. Tremblay Catrine Tudor‐Locke Pei Zhao Timothy S. Church for the ISCOLE Research Group 《Obesity (Silver Spring, Md.)》2015,23(8):1696-1702
89.
?ngela Moreira Sofia S. Pereira Madalena Costa Tiago Morais Ana Pinto Rúben Fernandes Mariana P. Monteiro 《PloS one》2015,10(4)
Obesity has been associated with increased incidence and risk of mortality of prostate cancer. One of the proposed mechanisms underlying this risk association is the change in adipokines expression that could promote the development and progression of the prostate tumor cells. The main goal of this study was to evaluate the effect of preadipocyte and adipocyte secretome in the proliferation, migration and invasion of androgen independent prostate carcinoma cells (RM1) and to assess cell proliferation in the presence of the adiposity signals leptin and insulin. RM1 cells were co-cultured in with preadipocytes, adipocytes or cultured in their respective conditioned medium. Cell proliferation was assessed by flow cytometry and XTT viability test. Cell migration was evaluated using a wound healing injury assay of RM1 cells cultured with conditioned media. Cellular invasion of RM1 cells co-cultured with adipocytes and preadipocytes was assessed using matrigel membranes. Preadipocyte conditioned medium was associated with a small increase in RM1 proliferation, while adipocytes conditioned media significantly increased RM1 cell proliferation (p<0.01). Adipocytes also significantly increased the RM1 cells proliferation in co-culture (p <0.01). Cell migration was higher in RM1 cells cultured with preadipocyte and adipocyte conditioned medium. RM1 cell invasion was significantly increased after co-culture with preadipocytes and adipocytes (p <0.05). Insulin also increased significantly the cell proliferation in contrast to leptin, which showed no effect. In conclusion, prostate carcinoma cells seem to be influenced by factors secreted by adipocytes that are able to increase their ability to proliferate, migrate and invade. 相似文献
90.
Directed cell migration is a crucial orchestrated process in embryonic development, wound healing, and immune response. The underlying substrate can provide physical and/or chemical cues that promote directed cell migration. Here, using electrospinning we developed substrates of aligned poly(lactic-co-glycolic acid) nanofibres to study the influence of glial cells on endothelial cells (ECs) in a 3-dimensional (3D) co-culture model. ECs build blood vessels and regulate their plasticity in coordination with neurons. Likewise, neurons construct nerves and regulate their circuits in coordination with ECs. In our model, the neuro-vascular cross-talk was assessed using a direct co-culture model of human umbilical vein endothelial cells (HUVECs) and rat Schwann cells (rSCs). The effect of rSCs on ECs behavior was demonstrated by earlier and higher velocity values and genetic expression profiles different of those of HUVECs when seeded alone. We observed 2 different gene expression trends in the co-culture models: (i) a later gene expression of angiogenic factors, such as interleukin-8 (IL-8) and vascular endothelial growth factor (VEGF), and (ii) an higher gene expression of genes involved in actin filaments rearrangement, such as focal adhesion kinase (FAK), Mitogen-activated protein kinase-activated protein kinase 13 (MAPKAPK13), Vinculin (VCL), and Profilin (PROF). These results suggested that the higher ECs migration is mainly due to proteins involved in the actin filaments rearrangement and in the directed cell migration rather than the effect of angiogenic factors. This co-culture model provides an approach to enlighten the neurovascular interactions, with particular focus on endothelial cell migration. 相似文献