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131.
The sequences of the linked alpha 2- and alpha 1-globin genes of the equine
BI and BII haplotypes are greater than 99% identical within a 1.2-kb region
extending from approximately 75 bp upstream of the putative cap site to a
point approximately 150 bp 3' to the poly A addition signal. Differences
between the alpha 2 and alpha 1 genes that are common to both haplotypes
indicate that a major gene conversion occurred approximately 12 Myr ago and
that this has been followed by shorter, more localized, conversions.
Interhaplotype (allelic) comparisons at the alpha loci suggest that the BI
and BII haplotypes have probably existed independently greater than or
equal to 0.5 Myr and that the alpha 1 genes may have undergone a recent
interchromosomal gene conversion.
相似文献
132.
A procedure for isolation of subpopulations of highly motile spermatozoa from ejaculates of several mammalian species was modified for use with boar semen. An initial trial was conducted to test the effectiveness of concentrations of 3 to 15% Bovine Serum Albumin (BSA) as an isolation medium. A concentration of 15% BSA resulted in isolation of a population of boar spermatozoa with 70% motility compared with an estimate of 53.5% determined after collection. Although there was a variable effect of isolation on the rate of forward movement, sperm cell concentration decreased (P<.05) as BSA level increased.To test the fertilizing capacity of isolated boar spermatozoa, 16 sows were randomly alloted to either a treated group bred with isolated spermatozoa (12% BSA) or control group bred with raw semen. Both the percent motile spermatozoa (64.1%) and the rate of forward movement (2.9) were increased (P<.01) after isolation in BSA compared with initial values obtained at collection (51.4% and 2.3). Farrowing rates (71.4% and 75% for treated and control groups, respectively) were not significantly different, and the sex ratio of the offspring was not altered by the procedure. 相似文献
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Phosphofructokinase isozymes of fetal, neonatal, and adult rat heart and skeletal muscle were characterized by DEAE-cellulose chromatography, agarose gel electrophoresis, and immunodiffusion with specific antisera. The results of these studies indicate that in skeletal muscle and heart the levels of the major liver phosphofructokinase isozyme (PFK-L2) and the muscle phosphofructokinase isozyme (PFK-M) are dependent on the developmental status of the rat. For example, PFK-L2 and PFK-M are present in fetal and early neonatal skeletal muscle; whereas in adult skeletal muscle, only PFK-M is detectable. By DEAE- cellulose chromatography, PFK-L2 activity was estimated to be 2.4 units/g (41% of total phosphofructokinase activity) in fetal muscle, very low and not resolved from PFK-M in 7-day neonatal muscle, and not detectable in adult muscle. Further, PFK-M activity was found to be 3.4 units/g (59% of total phosphofructokinase activity), 10 units/g, and 31.6 units/g in fetal, 7-day neonatal, and adult skeletal muscle, respectively. The developmental changes of heart phosphofructokinase isozymes differ considerably from that of the skeletal muscle phosphofructokinase isozymes. In fetal heart, PFK-L2 is the major phosphofructokinase isozyme (5.6 units/g), constituting 67% of total phosphofructokinase activity. Further, in fetal heart another phosphofructokinase isozyme (33% of total phosphofructokinase activity) was found by DEAE-cellulose chromatography which is different from PFK-M and PFK-L2. In 7-day neonatal and adult heart, PFK-M and PFK-L2 are the only detectable phosphofructokinase isozymes. Varying from 5.6 units/g (44% of total) in 7-day neonatal to 5.9 units/g (40% of total) in adult heart, PFK-L2 activity remains fairly constant. Also, PFK-M is very low in fetal heart but increases within 1 week postpartum to 5.5 units/g (50% of total activity) and to 8.9 units/g (60% of total activity) in adult heart. 相似文献