全文获取类型
收费全文 | 111619篇 |
免费 | 9347篇 |
国内免费 | 159篇 |
专业分类
121125篇 |
出版年
2022年 | 635篇 |
2021年 | 1368篇 |
2020年 | 949篇 |
2019年 | 1154篇 |
2018年 | 1803篇 |
2017年 | 1655篇 |
2016年 | 2703篇 |
2015年 | 4264篇 |
2014年 | 4666篇 |
2013年 | 5721篇 |
2012年 | 7335篇 |
2011年 | 6976篇 |
2010年 | 4345篇 |
2009年 | 3695篇 |
2008年 | 5755篇 |
2007年 | 5845篇 |
2006年 | 5632篇 |
2005年 | 5298篇 |
2004年 | 5208篇 |
2003年 | 4798篇 |
2002年 | 4716篇 |
2001年 | 2458篇 |
2000年 | 2395篇 |
1999年 | 2153篇 |
1998年 | 1329篇 |
1997年 | 973篇 |
1996年 | 877篇 |
1995年 | 821篇 |
1994年 | 867篇 |
1993年 | 816篇 |
1992年 | 1448篇 |
1991年 | 1284篇 |
1990年 | 1253篇 |
1989年 | 1212篇 |
1988年 | 1096篇 |
1987年 | 1010篇 |
1986年 | 997篇 |
1985年 | 940篇 |
1984年 | 929篇 |
1983年 | 850篇 |
1982年 | 816篇 |
1981年 | 771篇 |
1980年 | 685篇 |
1979年 | 818篇 |
1978年 | 687篇 |
1977年 | 605篇 |
1976年 | 533篇 |
1975年 | 581篇 |
1974年 | 604篇 |
1973年 | 547篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
101.
102.
103.
104.
We are investigating the mechanisms for producing or avoiding errors during DNA synthesis catalyzed by DNA replication and repair proteins purified from eukaryotic sources. Using assays that monitor the fidelity of a single round of DNA synthesis in vitro, we have defined the error frequency and mutational specificity of the four classes of animal cell DNA polymerases (alpha, beta, delta, gamma), and the fidelity of an SV40 origin-dependent DNA replication complex in extracts of HeLa cells. 相似文献
105.
Direct selection for curing and deletion of Rhizobium plasmids using transposons carrying the Bacillus subtilis sacB gene 总被引:13,自引:0,他引:13
We have constructed derivatives of the transposon Tn5 carrying the mob site (oriT) of plasmid RP4, and an nptI-sacB-sacR cassette [Ried and Collmer, Gene 57 (1987) 239-246]. The mob site, in conjunction with the antibiotic-resistance markers carried on the transposons, allows identification of transposon inserts in cryptic plasmids by mobilisation to other strains. The sacB-sacR genes allow direct selection for the loss or curing of plasmids, because only strains which no longer contain an active sacB gene are able to grow on media containing sucrose. We have tested these transposons in four strains of Rhizobium leguminosarum and two strains of Rhizobium meliloti, and have been able to demonstrate curing of several large cryptic plasmids, and generation of large deletions in many other plasmids. This method has enabled us to show that the R. leguminosarum plasmids pRL12JI and pR1eVF39f carry auxotrophic markers, and that the plasmid pR1eVF39c carries genes which affect colony morphology. 相似文献
106.
William S. Bowie Thomas K. Hunt Hubert A. Allen Jr 《The Western journal of medicine》1988,149(2):172-177
This study was presented in part at the annual meeting of the Wilderness Medical Society at Aspen, Colorado, in August 1986 and at “Mountain Medicine 1987,” Leavenworth, Washington, in November 1987.We questioned 220 injured rock climbers or their partners seen consecutively at the Yosemite (California) Medical Clinic over 3 ½ years regarding details of their accidents. Injury type and location were extracted from medical records and severity quantified. The National Park Service rescued 27% of the climbers. Injured climbers were characteristically male (88%) and experienced (mean 5.9 years) and typically fell while leading climbs (66%). Among 451 injuries, 50% were to the skin or subcutaneous tissues, while 28% involved the lower extremity and were predominantly fractures. In terms of each climber''s most severe injury (n = 220), 45% involved the lower extremities (30% from the ankle alone). Head injury or hypothermia caused 12 of 13 fatalities, showing the lowest case-fatality rate reported to date among injured climbers (6%). Rescue personnel successfully managed airways in victims of head injuries, anticipated and treated complications of hypothermia, and stabilized fractures. Victims requiring immediate extensive surgical intervention or blood transfusion usually died before rescue could be effected. 相似文献
107.
H. Wieler H. W. Hoffmanns A. Kippels S. Huhndorf A. Düx 《Radiation and environmental biophysics》1987,26(3):219-226
Summary The simultaneous use of intraarterial Cis-Platinum and Radiotherapy (CP/RT) was found to be a very effective and relatively little burdened treatment for a palliative treatment concept. This affects life quality as well as the remission - and survival times. The fast and continual remission with low CP/RT concentrations, even in extreme palliative cases, is surprising. CP/RT treatment shows additive and synergistic effects which are not explainable by the single effects of the cis-platinum dose used (60 mg/1.73 m2 in our case) or the total irradiation dose (e.g., 5 Gy TD) or the fractionation (e.g., 5 × 1 Gy), especially since the doses of each which were used are by themselves without therapeutic relevance. Only the combination of the modalities with a low dose two-day preradiation program induced the described effects.Dedicated to Prof. L.E. Feinendegen on the occasion of his 60th birthday 相似文献
108.
109.
110.