全文获取类型
收费全文 | 150649篇 |
免费 | 5964篇 |
国内免费 | 832篇 |
出版年
2023年 | 243篇 |
2022年 | 504篇 |
2021年 | 1006篇 |
2020年 | 632篇 |
2019年 | 808篇 |
2018年 | 12620篇 |
2017年 | 11325篇 |
2016年 | 8788篇 |
2015年 | 3010篇 |
2014年 | 2972篇 |
2013年 | 3433篇 |
2012年 | 8331篇 |
2011年 | 16626篇 |
2010年 | 14315篇 |
2009年 | 10381篇 |
2008年 | 12926篇 |
2007年 | 14497篇 |
2006年 | 3498篇 |
2005年 | 3580篇 |
2004年 | 3970篇 |
2003年 | 3772篇 |
2002年 | 3457篇 |
2001年 | 1124篇 |
2000年 | 869篇 |
1999年 | 837篇 |
1998年 | 862篇 |
1997年 | 610篇 |
1996年 | 522篇 |
1995年 | 472篇 |
1994年 | 505篇 |
1993年 | 523篇 |
1992年 | 643篇 |
1991年 | 538篇 |
1990年 | 493篇 |
1989年 | 501篇 |
1988年 | 460篇 |
1987年 | 422篇 |
1986年 | 405篇 |
1985年 | 378篇 |
1984年 | 443篇 |
1983年 | 415篇 |
1982年 | 426篇 |
1981年 | 383篇 |
1980年 | 396篇 |
1979年 | 312篇 |
1978年 | 299篇 |
1977年 | 263篇 |
1976年 | 259篇 |
1972年 | 403篇 |
1971年 | 438篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
41.
42.
David Skelly 《BMC biology》2010,8(1):136
A new study of divergence in freshwater fish provides strong evidence of rapid, temperature-mediated adaptation. This study
is particularly important in the ongoing debate over the extent and significance of evolutionary response to climate change
because divergence has occurred in relatively few generations in spite of ongoing gene flow and in the aftermath of a significant
genetic bottleneck, factors that have previously been considered obstacles to evolution. Climate change may thus be more likely
to foster contemporary evolutionary responses than has been anticipated, and I argue here for the importance of investigating
their possible occurrence. 相似文献
43.
The effects of mammalian gonadotropins, methyltestosterone and partially purified salmon gonadotropin on spermiation and oocyte maturation were studied in adult grey mullet. Methyltestosterone was a potent spermiating agent in both prespawning and spawning trials. The most effective dose was 5 mg/100 g body weight. Human chorionic gonadotropin (HCG) was moderately effective at a dose rate of 20 IU/100 g body weight. Both hormones blocked resorption of milt in captive animals. The HCG, FSH/LH combination, Synahorin+mullet pituitary homogenate, and partially purified salmon gonadotropin were equally effective, at the doses used, in inducing oocyte maturation and preventing onset of atrophy before completion of vitellogenesis. Atrophy occurred in all experimental fish at mean oocyte diameter of 750 μ. The significance of this finding is discussed. (Oceanic Institute Contribution No. 98). 相似文献
44.
45.
Transcriptional regulatory network triggered by oxidative signals configures the early response mechanisms of japonica rice to chilling stress 总被引:3,自引:0,他引:3
46.
Recent studies have revealed an unexpected synergism between two seemingly unrelated protein families: CCN matricellular proteins
and the tumor necrosis factor (TNF) family of cytokines. CCN proteins are dynamically expressed at sites of injury repair
and inflammation, where TNF cytokines are also expressed. Although TNFα is an apoptotic inducer in some cancer cells, it activates
NFκB to promote survival and proliferation in normal cells, and its cytotoxicity requires inhibition of de novo protein synthesis
or NFκB signaling. The presence of CCN1, CCN2, or CCN3 overrides this requirement and unmasks the apoptotic potential of TNFα,
thus converting TNFα from a proliferation-promoting protein into an apoptotic inducer. These CCN proteins also enhance the
cytotoxicity of other TNF cytokines, including LTα, FasL, and TRAIL. Mechanistically, CCNs function through integrin α6β1 and the heparan sulfate proteoglycan (HSPG) syndecan-4 to induce reactive oxygen species (ROS) accumulation, which is essential
for apoptotic synergism. Mutant CCN1 proteins defective for binding α6β1-HSPGs are unable to induce ROS or apoptotic synergism with TNF cytokines. Further, knockin mice that express an α6β1-HSPG-binding defective CCN1 are blunted in TNFα- and Fas-mediated apoptosis, indicating that CCN1 is a physiologic regulator
of these processes. These findings implicate CCN proteins as contextual regulators of the inflammatory response by dictating
or enhancing the cytotoxicity of TNFα and related cytokines. 相似文献
47.
Background
Introductions of non-native tiger salamanders into the range of California tiger salamanders have provided a rare opportunity to study the early stages of secondary contact and hybridization. We produced first- and second-generation hybrid salamanders in the lab and measured viability among these early-generation hybrid crosses to determine the strength of the initial barrier to gene exchange. We also created contemporary-generation hybrids in the lab and evaluated the extent to which selection has affected fitness over approximately 20 generations of admixture. Additionally, we examined the inheritance of quantitative phenotypic variation to better understand how evolution has progressed since secondary contact. 相似文献48.
49.
CHLOROPLASTS are more permeable to amino-acids1,2 than are cell membranes3 and also discriminate between their optical isomers2. This has led to the suggestion that the passage of amino-acids through the limiting membranes of chloroplasts is facilitated by carriers2. Using the additional criteria of saturation and competition, we support this suggestion and show that at least two carriers are probably involved. 相似文献
50.
W. Kelley Thomas J. T. Vida Linda M. Frisse Manuel Mundo James G. Baldwin 《Journal of nematology》1997,29(3):250-254
To effectively integrate DNA sequence analysis and classical nematode taxonomy, we must be able to obtain DNA sequences from formalin-fixed specimens. Microdissected sections of nematodes were removed from specimens fixed in formalin, using standard protocols and without destroying morphological features. The fixed sections provided sufficient template for multiple polymerase chain reaction-based DNA sequence analyses. 相似文献